FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002167641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8789863
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6740117.668048978692841No Hit
TCAGTGCACTACAGAACTTTGT2974063.3835112105842837No Hit
CGCGACCTCAGATCAGA2558792.911069262399198No Hit
AAACCGTTACCATTACTGAGC1319971.5016957602183334No Hit
TTCAAGTAATCCAGGATAGGCT1119031.2730915146231516No Hit
CGCGACCTCAGATC854860.9725521319274258No Hit
TGAGGTAGTAGGTTGTATAGT843130.9592072140373519No Hit
CCCATAAAGTAGAAAGCACT632460.7195333988709495No Hit
TGGCTCAGTTCAGCAGGAAC628020.7144821256030952No Hit
TGAGGTAGTAGGTTGTATAGTT624800.7108188148097416No Hit
TCAGTGCACTACAGAACTTTGC619780.70510769052942No Hit
TATTGCACTTGTCCCGGCCTGT612510.6968367993903887No Hit
TCCTGTACTGAGCTGCCCCGAG594770.6766544598021608No Hit
TTCACAGTGGCTAAGTTCCG586640.6674051688860224No Hit
TGGAGTGTGACAATGGTGTTT585310.6658920622539851No Hit
AAGCTGCCAGTTGAAGAACTGT566900.6449474809789413No Hit
AAACCGTTACCATTACTGAG546840.6221257373408436No Hit
TGAGGTAGTAGATTGTATAGT524000.5961412595395401No Hit
TCCTGTACTGAGCTGCCCCGA521940.5937976507711212No Hit
CGCGACCTCAGATCAGACGT515760.5867668244658648No Hit
TGAGGGGCAGAGAGCGAGACTTT497130.5655719548757472No Hit
TGAGGTAGTAGGTTGTGTGGT439860.5004173557653857No Hit
TAGCTTATCAGACTGATGTTGAC425890.48452404775819596No Hit
TGAGGTAGTAGATTGTATAGTT424590.4830450713509414No Hit
TGAGGGGCAGAGAGCGAGACT410110.46657154952244423No Hit
TAGCTTATCAGACTGATGTTGA369590.4204729925824782No Hit
TGAGGTAGTAGTTTGTACAGT326240.371154817771335No Hit
TGAGGTAGTAGTTTGTGCTGTT305960.3480827858181635No Hit
TGAGGTAGTAGGTTGTGTGGTT305470.34752532548004444No Hit
TGAGATGAAGCACTGTAGCT299330.34054000614116514No Hit
TCGTACCGTGAGTAATAATGCG279730.3182415926164037No Hit
CATTGCACTTGTCTCGGTCTGA271880.30931085046490486No Hit
TTCAAGTAATCCAGGATAGGCC265010.3014950289896441No Hit
TGAGGTAGTAGGTTGTATAG261460.29745628572367966No Hit
TAGCTTATCAGACTGATGTTG250540.2850328839027411No Hit
AAACCGTTACCATTACTG246180.28007262456764115No Hit
TTTGGAGCAGGGAGA228960.26048187554231506No Hit
TCCTGTACTGAGCTGCCCCG221310.25177866822270156No Hit
TGAGGTAGTAGGTTGTGTGG216070.24581725562730614No Hit
GGCTGGTCCGATGGTAGT215830.24554421382904376No Hit
TGAGATGAAGCACTGTAGCTC179430.2041328744259154No Hit
CGCGACCTCAGATCAGAC178630.20322273509837413No Hit
TGAGGTAGTAGGTTGTAT175390.1995366708218319No Hit
TGAGGGGCAGAGAGCGAGACTT170580.19406445811498996No Hit
TGAGGTAGTAGGTTGTATAGC168190.1913454168739604No Hit
TTTTTGGAGCAGGGAGA155480.17688557830764826No Hit
TATTGCACTTGTCCCGGCCTGC154980.17631674122793495No Hit
AAAAGCTGGGTTGAGAGGGCGA153740.17490602527024596No Hit
TTCAAGTAATTCAGGATAGGT151100.17190256548935973No Hit
CTAGACTGAAGCTCCTTGAGG144980.1649399996336689No Hit
TGAGGTAGTAGATTGTATAG144570.164473553228304No Hit
AGAAGCCGGCGGGAGC142600.1622323351342336No Hit
TGAGGTAGTAGATTGTAT142430.1620389305271311No Hit
AAGCTGCCAGTTGAAGAACT142000.16154973063857764No Hit
TGGCTCAGTTCAGCAGGAACAG140420.15975220546668362No Hit
AACCCGTAGATCCGATCTTGT138690.15778402917087558No Hit
TGAGAACTGAATTCCATGGGTT138100.15711280141681389No Hit
GGCTGGTCCGATGGTAGTG137480.15640744343796942No Hit
ATTTTTGGAGCAGGGAGA134780.15333572320751757No Hit
CGGACAGGATTGACAGA134360.15285790006055838No Hit
TGAGGTAGTAGGTTGTATAGTC132240.150446030842574No Hit
CCCATAAAGTAGAAAGCACC131540.14964965893097537No Hit
TGTAAACATCCCCGACTGGAAG131120.1491718357840162No Hit
TTCACAGTGGCTAAGTTCCA127070.14456425543833845No Hit
AAGCTGCCAGTTGAAGAACTGC123020.13995667509266072No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC121040.13770408025699604No Hit
CGCGACCTCAGATCAGACGC118390.13468924373451555No Hit
TCCTGTACTGAGCTGCCCCGAGA115340.1312193375482644No Hit
TGAGGTAGTAGTTTGTGCTGT113240.12883022181346854No Hit
TTCAAGTAATCCAGGATAGGCA110660.1258950224821479No Hit
TGAGGTAGTAGTTTGTACAGTT110570.1257926318077995No Hit
TGGAGTGTGACAATGGTGTTC110520.12573574809982818No Hit
TGTCTGAGCGTCGCT107510.12231134887995411No Hit
AAACCGTTACCATTACTGAGA106410.12105990730458484No Hit
GGCTGGTCCGATGGTAGTGGGT106380.12102577707980204No Hit
CATTGCACTTGTCTCGGTCTG106220.12084374921429379No Hit
AAACCGTTACCATTACTGAGTT106120.12072998179835112No Hit
GTCTGAGCGTCGCT104820.11925100539109655No Hit
TGAGGTAGTAGATTGTATAGC104500.11888694966008004No Hit
TCCTGTACTGAGCTGCCCCGAGT103700.11797681033253873No Hit
AACATTCAACGCTGTCGGTGA101390.11534878302426328No Hit
CGCGACCTCAGATT101380.11533740628266902No Hit
TGAGGTAGTAGGTTGTGTGGC98980.11260698830004517No Hit
TCAGTGCATCACAGAACTTTGT98010.11150344436540137No Hit
GGGTGCTGTAGGCTT97730.11118489560076193No Hit
AAATGGATTTTTGGAGCAGGGA94910.10797665447117889No Hit
CGCGACCTCAGATCAGACG94730.10777187312248213No Hit
TGGCTCAGTTCAGCAGGAACA93150.10597434795058809No Hit
TGAGGGGCAGAGAGCGAGACC91950.10460913895927615No Hit
TGAGGTAGTAGATTGTATAGTC90750.10324392996796422No Hit
AGCTCGGTCTGAGGCCCCTCAGT90010.10240205108998854No Hit
AGGAAACTCTGGTG89570.10190147445984085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)