FastQCFastQC Report
Wed 8 May 2024
EGAF00002167642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12828424
Total Bases296.7 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6174994.813521910407701No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC5264064.103434685351841No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3717762.8980644855517714No Hit
TCAGTGCACTACAGAACTTTGT3201272.4954507272288473No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1818001.4171655068463593No Hit
TTCAAGTAATCCAGGATAGGCT1721681.342082238628845No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1507891.1754288757527815No Hit
CGCGACCTCAGATCAGA1505201.1733319696947966No Hit
TAGCTTATCAGACTGATGTTGAC1353261.0548918557727744No Hit
TATTGCACTTGTCCCGGCCTGT1163290.9068066350161174No Hit
TCCTGTACTGAGCTGCCCCGAG1155170.9004769408931292No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1000930.7802439333155811No Hit
AAACCGTTACCATTACTGAGC951400.7416343582033148No Hit
TCCTGTACTGAGCTGCCCCGA917880.7155048819714721No Hit
CGCGACCTCAGATC873710.6810735285955625No Hit
TGGAGTGTGACAATGGTGTTT814660.635042932787379No Hit
TAGCTTATCAGACTGATGTTGA751490.5858007187788618No Hit
TGAGGGGCAGAGAGCGAGACTTT747830.582947679309633No Hit
TTCACAGTGGCTAAGTTCCG729550.5686980723431031No Hit
TGGCTCAGTTCAGCAGGAAC726050.5659697559107806No Hit
TGAGGTAGTAGGTTGTATAGTT699780.5454917922887488No Hit
AAACCGTTACCATTACTGAG696210.54270890952778No Hit
AAGCTGCCAGTTGAAGAACTGT665670.5189023998583145No Hit
TCGTACCGTGAGTAATAATGCG658110.513009236364498No Hit
TGAGGTAGTAGATTGTATAGTT641390.49997567900780326No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT617200.4811191148655517No Hit
CATTGCACTTGTCTCGGTCTGA590170.4600487168182155No Hit
TGAGGTAGTAGGTTGTATAGT553220.4312454904826969No Hit
CCCATAAAGTAGAAAGCACT529910.4130749030434292No Hit
TCAGTGCACTACAGAACTTTGC523870.40836660840022126No Hit
TAGCTTATCAGACTGATGTTG522920.4076260653685908No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC496400.38695322200139315No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC465580.3629284470173421No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG450550.35121227673796873No Hit
TGAGGTAGTAGTTTGTGCTGTT435930.33981570924066745No Hit
TGAAAAGAACTTTGAAGAGAG413840.3225961349578093No Hit
CGCGACCTCAGATCAGACGT406250.3166795858945729No Hit
TGAGGTAGTAGATTGTATAGT394120.30722402065912385No Hit
GCATTGGTGGTTCAGTGGTAGAATT375140.29242875040612937No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG343050.26741398631663565No Hit
TGAGGGGCAGAGAGCGAGACT338860.2641478017876553No Hit
TTCAAGTAATCCAGGATAGGCC322080.2510674732921207No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC319660.24918103735891484No Hit
TCCTGTACTGAGCTGCCCCG318420.24821443382289204No Hit
TGAGGTAGTAGTTTGTACAGT315600.24601619029742078No Hit
TGTAAACATCCCCGACTGGAAG310550.24207961944506978No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC306700.23907847136951507No Hit
TGAGGTAGTAGGTTGTGTGGTT299910.2337855374908095No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT297380.23181335446973064No Hit
TGAGGTAGTAGGTTGTGTGGT280260.21846798952077046No Hit
AAAAGCTGGGTTGAGAGGGCGA265210.2067362288617838No Hit
TCCTGTACTGAGCTGCCCCGAGA265000.20657252987584446No Hit
CGCGACCTCAGATCAGAC261730.20402350280907458No Hit
TGAGGTAGTAGGTTGTATAG253920.19793545957009218No Hit
TGGCTCAGTTCAGCAGGAACAG253830.19786530286183246No Hit
TGAGATGAAGCACTGTAGCTC238890.1862192892907188No Hit
TGTAAACATCCCCGACTGGAAGCT232320.18109784958775918No Hit
TGAGGTAGTAGGTTGTGTGG230460.17964794428372496No Hit
TGAGAACTGAATTCCATGGGTT226400.17648309722223088No Hit
AGGAAACTCTGGTG225900.1760933377318991No Hit
CTAGACTGAAGCTCCTTGAGG218610.17041064436286174No Hit
TGAGATGAAGCACTGTAGCT218110.17002088487252995No Hit
AAACCGTTACCATTACTG214780.16742508666692027No Hit
TATTGCACTTGTCCCGGCCTGC205780.16040941584094817No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT202940.1581955819358637No Hit
CAACGGAATCCCAAAAGCAGCTG199820.15576348271619336No Hit
CGCGACCTCAGATCAGACG193390.15075117567052665No Hit
TACAGTACTGTGATAACTGAAG191760.14948055973204505No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA187760.1463624838093908No Hit
TGTAAACATCCTTGACTGGAAGCT186130.14509186787090916No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA182390.14217646688322744No Hit
CATTGCACTTGTCTCGGTCTG180980.1410773451204918No Hit
TGTAAACATCCCCGACTGGAAGC174260.13583897757043265No Hit
GTTGAAAAGAACTTTGAAGAGAG172920.13479442213634346No Hit
TGAGGGGCAGAGAGCGAGACTT168320.1312086348252911No Hit
TCCTGTACTGAGCTGCCCCGAGT165490.1290025961100132No Hit
TATTGCACTTGTCCCGGCCTG162980.12704600346854766No Hit
GGCTGGTCCGATGGTAGTG160610.125198543484375No Hit
TCAGTGCATCACAGAACTTTGT158370.12345242096768862No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT157940.12311722780600329No Hit
TGAGGTAGTAGTTTGTGCTGT157630.12287557692199759No Hit
AGCTCGGTCTGAGGCCCCTCAGT157120.12247802224185918No Hit
TGAGGTAGTAGTTTGTACAGTT153980.12003033264257558No Hit
GGCTGGTCCGATGGTAGTGGGT153760.1198588384668296No Hit
GGCTGGTCCGATGGTAGTGGGTT150240.11711493165489385No Hit
TGAGGTAGTAGATTGTATAG149500.11653808760920281No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC149180.11628864153539048No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT149150.11626525596597057No Hit
TAGCAGCACGTAAATATTGGCG143350.1117440458781219No Hit
GGCTGGTCCGATGGTAGTGGGTTATC139320.10860258438604774No Hit
AACCCGTAGATCCGATCTTGT138640.1080725114791965No Hit
AAACCGTTACCATTACTGAGTT135800.10585867757411198No Hit
CGTGTATTTGACAAGCTGAGTTG135470.10560143631049301No Hit
GGCTGGTCCGATGGTAGT135290.10546112289397357No Hit
TACCTGGTTGATCCTGCC134290.10468160391331001No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA133740.10425286847394505No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC132460.1032550841786957No Hit
TTCAAGTAATTCAGGATAGGT130770.10193769710137425No Hit
TACCCTGTAGAACCGAATTTGT128460.10013700825604144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)