FastQCFastQC Report
Wed 8 May 2024
EGAF00002167645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10749629
Total Bases247 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10129089.42272519358575No Hit
TCAGTGCACTACAGAACTTTGT4681744.355257283763002No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1798441.67302518068298No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1606581.4945446024230231No Hit
TTCAAGTAATCCAGGATAGGCT1520301.4142813672918386No Hit
AAACCGTTACCATTACTGAGC1499201.3946527829006936No Hit
TATTGCACTTGTCCCGGCCTGT1339301.2459034632730115No Hit
TCCTGTACTGAGCTGCCCCGAG1030330.9584795903188845No Hit
TGAGGTAGTAGGTTGTATAGT961930.8948494873637034No Hit
TGAGGTAGTAGGTTGTATAGTT949430.8832211790751104No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC899410.8366893406274766No Hit
CGCGACCTCAGATCAGA821380.7641007889667634No Hit
TCAGTGCACTACAGAACTTTGC754980.7023312153377572No Hit
TAGCTTATCAGACTGATGTTGAC748730.6965170611934607No Hit
AAACCGTTACCATTACTGAG708400.6589994873311441No Hit
AAGCTGCCAGTTGAAGAACTGT702830.6538179131577471No Hit
TGAGGTAGTAGATTGTATAGTT681430.6339102493676758No Hit
TCCTGTACTGAGCTGCCCCGA653700.6081140102602611No Hit
AAACCGTTACCATTACTGAGG626820.5831084961164706No Hit
CCCATAAAGTAGAAAGCACT619280.5760943005567913No Hit
TGAGGTAGTAGATTGTATAGT616620.5736197965529788No Hit
TGAGGGGCAGAGAGCGAGACTTT600420.5585495090109621No Hit
TAGCTTATCAGACTGATGTTGA570690.5308927405773725No Hit
CATTGCACTTGTCTCGGTCTGA510450.474853597272985No Hit
TGAGGTAGTAGTTTGTGCTGTT470400.4375964975163329No Hit
TTCACAGTGGCTAAGTTCCG455170.42342856669751117No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC432180.40204178209313085No Hit
TCGTACCGTGAGTAATAATGCG422280.3928321619285652No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT420600.39126931729457826No Hit
TGAGGGGCAGAGAGCGAGACT413960.3850923599316776No Hit
TGAGGTAGTAGTTTGTACAGT403360.37523155450295076No Hit
AAACCGTTACCATTACTGAGTT395210.3676498974987881No Hit
TGAGGTAGTAGGTTGTGTGGT393760.3663010137373113No Hit
TGGCTCAGTTCAGCAGGAAC388500.3614078216094713No Hit
TGAGGTAGTAGGTTGTGTGGTT356790.33190912914296855No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT354230.3295276516054647No Hit
TAGCTTATCAGACTGATGTTG325360.30267091078213026No Hit
TGAGGTAGTAGGTTGTATAG305570.2842609730996298No Hit
TCAGTGCACTACAGAACTTTGG303550.2823818384801931No Hit
AAATGGATTTTTGGAGCAGGGAGA288020.26793482826244513No Hit
TTCAAGTAATCCAGGATAGGCC284250.2644277304826055No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC272670.2536552656840529No Hit
TATTGCACTTGTCCCGGCCTGC261260.2430409458782252No Hit
TCCTGTACTGAGCTGCCCCG259290.2412083244919429No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC256770.23886405754096257No Hit
AAAAGCTGGGTTGAGAGGGCGA247870.23058470203948436No Hit
TGGAGTGTGACAATGGTGTTT240740.2239519149916709No Hit
AAACCGTTACCATTACTG227300.21144915791977567No Hit
TGAGGTAGTAGTTTGTACAGTT226480.21068634089604393No Hit
TGGCTCAGTTCAGCAGGAACAG223340.2077653098539494No Hit
TGAGAACTGAATTCCATGGGTT220130.2047791602854387No Hit
TCCTGTACTGAGCTGCCCCGAGA219940.2046024099994521No Hit
TAGCAGCACGTAAATATTGGCG211980.19719750328127603No Hit
CTAGACTGAAGCTCCTTGAGG204960.1906670453464022No Hit
TGAGGTAGTAGGTTGTGTGG204080.18984841244288525No Hit
CGCGACCTCAGATCAGACGT198370.18453660121665594No Hit
CATTGCACTTGTCTCGGTCTG196680.18296445393603816No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT195750.18209930779936684No Hit
TCCTGTACTGAGCTGCCCCGAGT194900.18130858283574253No Hit
TGAGATGAAGCACTGTAGCT192750.17930851381010451No Hit
CGCGACCTCAGATC191830.17845267032006407No Hit
TGAGGTAGTAGTTTGTGCTGT190680.1773828659575135No Hit
AAATGGATTTTTGGAAATAGGAGA189860.1766200489337818No Hit
TGTAAACATCCCCGACTGGAAG180690.16808952197326996No Hit
TGTAAACATCCCCGACTGGAAGCT174010.16187535402384584No Hit
TTCAAGTAATTCAGGATAGGT171450.159493876486342No Hit
AAATGGATTTTTGGAGCAGGGAGAT168300.15656354279761656No Hit
TGTAAACATCCTTGACTGGAAGCT165700.1541448546735892No Hit
TGAGGGGCAGAGAGCGAGACTT163130.15175407448945447No Hit
TGAGGTAGTAGATTGTATAG157280.14631202621039294No Hit
ACGGCCCTGGCGGA152310.14168861083484835No Hit
ACGGCCCTGGCGGAGC151150.14060950382566692No Hit
TCAGTGCATCACAGAACTTTGT147290.13701868222614938No Hit
TTCAAGTAATCCAGGATAGGCG146670.13644191813503517No Hit
AAACCGTTACCATTACTGAGA146500.1362837731423103No Hit
TGAGGTAGTAGGTTGTATAGC145060.13494419202746438No Hit
TGAGATGAAGCACTGTAGCTC144520.13444184910939716No Hit
TGAGGTAGTAGGTTGTATAGTC143360.13336274210021573No Hit
AAATGGATTTTTGGAGCAGGGAG137400.1278183647082146No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT136980.12742765354971786No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC135430.12598574332193233No Hit
AGCTCGGTCTGAGGCCCCTCAGT127480.11859013925038715No Hit
CGTGTATTTGACAAGCTGAGTTG126510.11768778252719232No Hit
TACAGTACTGTGATAACTGAAG124240.11557608174198385No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT121920.11341786772362099No Hit
CAACGGAATCCCAAAAGCAGCTG121150.11270156393304363No Hit
TATTGCACTTGTCCCGGCCTG118980.11068288961414388No Hit
AAGCTGCCAGTTGAAGAACTGC118820.1105340472680499No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT117110.10894329469417038No Hit
AGAGGTAGTAGGTTGCATAGTT116500.10837583324968703No Hit
AAATGGATTTTTGGAGCAGGGA113440.10552922338063946No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG112660.10480361694343125No Hit
TAGCTTATCAGACTGATGTTGAT112530.1046826825372299No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT110910.10317565378302823No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG110100.1024221394059274No Hit
GCATTGGTGGTTCAGTGGTAGAATT108930.10133372975011509No Hit
AGGAAACTCTGGTG108860.10126861122369897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)