FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16357225
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT16060389.818523618767854No Hit
TCAGTGCACTACAGAACTTTGT3937982.4074865999581223No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2941161.7980800533097758No Hit
AAACCGTTACCATTACTGAGC2686601.6424546339614452No Hit
TTCAAGTAATCCAGGATAGGCT2459211.5034396115478024No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2326421.422258359837931No Hit
TATTGCACTTGTCCCGGCCTGT1631860.9976386581464766No Hit
CGCGACCTCAGATCAGA1567090.9580414770842853No Hit
TCCTGTACTGAGCTGCCCCGAG1566820.9578764124110293No Hit
TGAGGTAGTAGGTTGTATAGTT1451360.887289867321627No Hit
TGAGGTAGTAGGTTGTATAGT1365220.8346281230465438No Hit
AAACCGTTACCATTACTGAGG1319390.8066099231379406No Hit
TGAGGTAGTAGATTGTATAGTT1251980.7653987763816906No Hit
TAGCTTATCAGACTGATGTTGAC1019810.6234614979007748No Hit
TGAGGTAGTAGATTGTATAGT935390.5718512767293963No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC890420.5443588383726456No Hit
TGAGGTAGTAGTTTGTACAGT879950.5379579971541627No Hit
TCCTGTACTGAGCTGCCCCGA875310.5351213301767261No Hit
AAACCGTTACCATTACTGAG843200.5154908610720951No Hit
AAGCTGCCAGTTGAAGAACTGT811220.4959398675508835No Hit
TGAGGTAGTAGTTTGTGCTGTT804720.4919660883799055No Hit
TGAGGTAGTAGGTTGTGTGGTT778200.4757530693623154No Hit
CCCATAAAGTAGAAAGCACT743780.4547103802753829No Hit
TCAGTGCACTACAGAACTTTGC711250.4348231439012424No Hit
CGCGACCTCAGATCAGACGT692630.4234397949529948No Hit
TGAGGTAGTAGGTTGTGTGGT678120.4145690971420886No Hit
CGCGACCTCAGATC676280.413444211961381No Hit
CATTGCACTTGTCTCGGTCTGA648630.3965403667186824No Hit
TAGCTTATCAGACTGATGTTGA646370.39515871426846544No Hit
TGAGGTAGTAGTTTGTACAGTT633380.38721726943292645No Hit
TGGAGTGTGACAATGGTGTTT613890.37530204542640944No Hit
TCGTACCGTGAGTAATAATGCG573450.3505790254765096No Hit
TGAGGTAGTAGGTTGTATAG565230.34555372320182676No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC534290.32663853434797163No Hit
TTCAAGTAATCCAGGATAGGCC527930.3227503442668301No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC500820.3061766283706436No Hit
TGAGGGGCAGAGAGCGAGACTTT485580.29685964459130443No Hit
TCCTGTACTGAGCTGCCCCGAGT435700.2663654745838613No Hit
TAGCTTATCAGACTGATGTTG435650.2663349070517768No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT432260.26426242837645136No Hit
TCCTGTACTGAGCTGCCCCGAGA431120.263565488644926No Hit
TTCAAGTAATTCAGGATAGGT411710.2516991726897441No Hit
TCCTGTACTGAGCTGCCCCG376730.2303141272434658No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT372590.2277831355868737No Hit
AAACCGTTACCATTACTGAGTT371910.22736741715052522No Hit
TATTGCACTTGTCCCGGCCTGC365250.22329582187687702No Hit
GCATTGGTGGTTCAGTGGTAGAATT357020.21826440609577724No Hit
AAACCGTTACCATTACTG355670.21743908272949722No Hit
TGTAAACATCCCCGACTGGAAGCT353090.2158617980739398No Hit
TTCACAGTGGCTAAGTTCCG352870.2157273009327682No Hit
TCAGTGCACTACAGAACTTTGG335960.20538936158180865No Hit
TTCAAGTAATCCAGGATAGGCG320200.19575447546879132No Hit
TGAGGGGCAGAGAGCGAGACT317860.19432391496723925No Hit
AAAAGCTGGGTTGAGAGGGCGA316300.19337020796620455No Hit
TGGCTCAGTTCAGCAGGAAC312130.19082087579036175No Hit
TGAGGTAGTAGTTTGTGCTGT309190.18902350490379635No Hit
TGAGGTAGTAGATTGTATAG302140.18471348287988945No Hit
TGGAGAGAAAGGCAGTTCCTGA299500.18309951718583073No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT296300.1811431951324262No Hit
TGAGAACTGAATTCCATGGGTT294340.1799449478747159No Hit
CAACGGAATCCCAAAAGCAGCTG283280.1731834097776365No Hit
CATTGCACTTGTCTCGGTCTG272850.16680702258482108No Hit
CAACGGAATCCCAAAAGCAGCT262370.16040006785992122No Hit
TGAGGTAGTAGGTTGTATAGTC258520.1580463678894189No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG255340.15610227284884814No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT254170.1553869925980721No Hit
GTTTCCGTAGTGTAGTGGTTATC253000.15467171234729607No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT250770.1533084004163298No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC250390.153076087172488No Hit
AAACCGTTACCATTACTGAGA247130.15108308408058213No Hit
TGAAAAGAACTTTGAAGAGAG231960.14180889484616124No Hit
TGTAAACATCCTTGACTGGAAGCT231540.14155212757665192No Hit
TGAGGTAGTAGGTTGTATAGC229060.1400359779852634No Hit
TGTAAACATCCCCGACTGGAAG223740.13678359257147835No Hit
CTAGACTGAAGCTCCTTGAGG223250.1364840307570508No Hit
AGAGGTAGTAGGTTGCATAGTT217560.13300544560584085No Hit
TGAGGTAGTAGATTGTATAGTC215640.13183165237379812No Hit
TTCAAGTAATCCAGGATAGGCA210590.1287443316332691No Hit
TGAGATGAAGCACTGTAGCT203140.12418976935268666No Hit
TACAGTACTGTGATAACTGAAG201550.12321772183240129No Hit
TGAGGTAGTAGGTTGTGTGG200220.12240462547895503No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT197810.12093127043248472No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG196660.12022821719454248No Hit
AGAGGTAGTAGGTTGCATAGT194250.11875486214807218No Hit
TATTGCACTTGTCCCGGCCTG191190.11688412918450408No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA188650.11533129855461424No Hit
TGGCTCAGTTCAGCAGGAACAG188400.11517846089419202No Hit
CGCGACCTCAGATCAGACG178520.10913831655430552No Hit
TGAGATGAAGCACTGTAGCTC177960.10879596019495973No Hit
TTCAAGTAATTCAGGATAGGTT177150.10830076617519169No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT174780.10685186515438896No Hit
TGAGGTAGTAGGTTGTAT173150.10585536360843603No Hit
CGCGACCTCAGATCAGAC172530.1054763262105889No Hit
AGCTCGGTCTGAGGCCCCTCAGT164460.10054272653215934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)