FastQCFastQC Report
Wed 8 May 2024
EGAF00002167647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181291
Total Bases198.1 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT97928510.666092600702886No Hit
AAACCGTTACCATTACTGAGC2040752.2227266296210413No Hit
TCAGTGCACTACAGAACTTTGT1824181.9868447694338411No Hit
TTCAAGTAATCCAGGATAGGCT1662171.8103881033723908No Hit
TATTGCACTTGTCCCGGCCTGT1403441.528586774997111No Hit
CGCGACCTCAGATCAGA1327841.4462454136351848No Hit
TAGCTTATCAGACTGATGTTGAC1109201.2081089685535509No Hit
TCCTGTACTGAGCTGCCCCGAG1056801.1510363847524276No Hit
TCCTGTACTGAGCTGCCCCGA808840.8809654328568826No Hit
AAGCTGCCAGTTGAAGAACTGT785000.8549995855702646No Hit
TGAGGTAGTAGTTTGTGCTGTT635560.6922338045924042No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC625830.6816361664171193No Hit
TAGCTTATCAGACTGATGTTGA599790.6532741419480115No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC598920.6523265627894814No Hit
TGAGGGGCAGAGAGCGAGACTTT587650.6400516005864535No Hit
TGAGGTAGTAGGTTGTATAGTT546070.5947638518373941No Hit
TGAGGTAGTAGGTTGTGTGGT538240.5862356394106232No Hit
TGAGGTAGTAGGTTGTATAGT535170.5828918830696032No Hit
TGAGGTAGTAGATTGTATAGTT525410.5722615697509206No Hit
TGAGGTAGTAGGTTGTGTGGTT504760.549770179378913No Hit
CCCATAAAGTAGAAAGCACT486440.5298165584774516No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT454650.4951917981904723No Hit
CGCGACCTCAGATC451640.4919133921362475No Hit
TGAGGGGCAGAGAGCGAGACT442770.48225244140502677No Hit
TTCAAGTAATCCAGGATAGGCC419350.4567440461259751No Hit
TGAGGTAGTAGTTTGTACAGT417420.4546419452340635No Hit
TCAGTGCACTACAGAACTTTGC401020.4367795335100478No Hit
TCCTGTACTGAGCTGCCCCGAGT389520.42425406187430503No Hit
TGAGGTAGTAGATTGTATAGT388840.42351342529062636No Hit
CGCGACCTCAGATCAGACGT384060.4183071857759437No Hit
TATTGCACTTGTCCCGGCCTGC367550.40032496519280353No Hit
AAACCGTTACCATTACTGAG367430.40019426461921315No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT340470.37083020241924586No Hit
TAGCTTATCAGACTGATGTTG328460.3577492533457441No Hit
TCCTGTACTGAGCTGCCCCGAGA323540.35239052982853936No Hit
CATTGCACTTGTCTCGGTCTGA313490.34144435679034685No Hit
TGTAAACATCCCCGACTGGAAGCT304160.3312823871936964No Hit
TCGTACCGTGAGTAATAATGCG291230.31719940038933525No Hit
TGAGGTAGTAGTTTGTGCTGT285270.31070793856768075No Hit
TAGCTTATCAGACTGATGTTGAT283420.308692971391496No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG281630.30674335450210655No Hit
TCCTGTACTGAGCTGCCCCG276820.3015044398440263No Hit
TGGCTCAGTTCAGCAGGAAC248550.27071356304903094No Hit
AGGAAACTCTGGTG247240.2692867484540028No Hit
TACAGTACTGTGATAACTGAAG236250.25731675425601913No Hit
TGAGGTAGTAGGTTGTGTGG224890.2449437666227985No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC216000.23526103246264604No Hit
AAACCGTTACCATTACTG210300.229052755217104No Hit
TGTAAACATCCTTGACTGGAAGCT203810.22198403252875876No Hit
TTCACAGTGGCTAAGTTCCG202330.22037205878781102No Hit
AAACCGTTACCATTACTGAGTT200200.21805212360658213No Hit
TACAGTATAGATGATGTACT188610.2054286265406466No Hit
TGAGGTAGTAGGTTGTATAG187540.20426321309279927No Hit
GCATTGGTGGTTCAGTGGTAGAATT184280.20071251417692784No Hit
TTCAAGTAATTCAGGATAGGT183870.20026595388382742No Hit
TGAGATGAAGCACTGTAGCTC178300.19419926892634162No Hit
CATTGCACTTGTCTCGGTCTG177000.1927833460457794No Hit
ACCAATATTACTGTGCTGCTT175790.19146544859540995No Hit
AAGCTGCCAGTTGAAGAACTGC173520.188993029411659No Hit
TGTAAACATCCCCGACTGGAAG166770.1816411221472013No Hit
GGCTGGTCCGATGGTAGT166660.1815213132880768No Hit
TACAGTACTGTGATAACTGA163200.1777527800828881No Hit
CGCGACCTCAGATCAGAC161800.1762279400576673No Hit
AAGCTGCCAGTTGAAGAACT157060.1710652674008481No Hit
AAACCGTTACCATTACTGAGA152320.1659025947440289No Hit
TCAGTGCATCACAGAACTTTGT151650.16517284987481606No Hit
CTAGACTGAAGCTCCTTGAGG150180.1635717678483342No Hit
TGAGGTAGTAGTTTGTACAGTT148210.16142610009855912No Hit
AAAAGCTGGGTTGAGAGGGCGA147620.1607834889450732No Hit
TGAGGTAGTAGTTTGTGCTGTC147490.16064189665701697No Hit
TGAGGGGCAGAGAGCGAGACTT138450.15079578677987662No Hit
TATTGCACTTGTCCCGGCCTG133940.14588362355577228No Hit
TGAGATGAAGCACTGTAGCT133310.1451974455444229No Hit
TGGAGTGTGACAATGGTGTTT130980.14265967607387675No Hit
TGTAAACATCCCCGACTGGAAGC128110.13953375402217402No Hit
TGAGGTAGTAGGTTGTGTGGC124890.13602662196416604No Hit
TGAGGTAGTAGGTTGTATAGTC123390.13439286479428655No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT123320.13431662279302553No Hit
TGAGGTAGTAGGTTGTGTGGTC122090.13297694191372433No Hit
TTCAAGTAATCCAGGATAGGCA121260.13207292961305767No Hit
TGAGAACTGAATTCCATGGGTT121110.13190955389606973No Hit
AGCTCGGTCTGAGGCCCCTCAGT120840.13161547760549142No Hit
TACAGTACTGTGATAACTGAA119040.12965496900163603No Hit
TGAAAAGAACTTTGAAGAGAG118790.12938267613998947No Hit
TGAGGTAGTAGATTGTATAGTC116110.12646369666313811No Hit
TCCTGTACTGAGCTGCCCCGAA114520.12473191406306586No Hit
TGAGGTAGTAGGTTGTATAGC113670.12380611833346748No Hit
TAGTGGGTTATCAGAACT113130.12321796575231085No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC112680.12272783860134702No Hit
TGAGGGGCAGAGAGCGAGACTTC111570.1215188582956362No Hit
CCCATAAAGTAGAAAGCACC109460.11922070654333905No Hit
GGCTGGTCCGATGGTAGTG107460.11704236365016642No Hit
TGAGGGGCAGAGAGCGAGACC106250.11572446619979697No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC104260.11355701502109017No Hit
TGAGGTAGTAGATTGTATAG103430.11265300272042353No Hit
TGAGGTAGTAGATTGTAT102870.11204306671033519No Hit
AGTTGGTCCGAGTGTTGT102340.11146580584364442No Hit
TGAGGTAGTAGGTTGTAT101150.1101696918222067No Hit
GGCTGGTCCGATGGTAGTGGGT98320.10708733662836742No Hit
GGGTGCTGTAGGCTT96480.10508326116664857No Hit
TGTCTGAGCGTCGCT96100.10466937601694576No Hit
AAACCGTTACCATTACTGAGG94500.10292670170240766No Hit
ACCAATATTACTGTGCTGCTTT93830.10219695683319481No Hit
TAGCTTATCAGACTGATGTTGACT93620.10196823082941169No Hit
CATTGCACTTGTCTCGGTCT92850.10112956881554022No Hit
TGAGGTAGTAGTTTGTGCTG92660.10092262624068882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)