FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10812532
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9423958.71576611287717No Hit
TCAGTGCACTACAGAACTTTGT2926982.7070255144678415No Hit
TTCAAGTAATCCAGGATAGGCT1911141.7675230926484196No Hit
TATTGCACTTGTCCCGGCCTGT1844561.705946396274249No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1838351.7002030606707106No Hit
TCCTGTACTGAGCTGCCCCGAG1722901.593428810199128No Hit
AAACCGTTACCATTACTGAGC1653711.5294382481365143No Hit
TCCTGTACTGAGCTGCCCCGA1379991.2762875522588049No Hit
TGAGGGGCAGAGAGCGAGACTTT1292001.1949097584173622No Hit
AAGCTGCCAGTTGAAGAACTGT1242911.1495087367140278No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1227791.1355249630706297No Hit
TAGCTTATCAGACTGATGTTGAC1114551.0307946371858137No Hit
TGAGGGGCAGAGAGCGAGACT1039650.9615231658967575No Hit
TGAGGTAGTAGGTTGTATAGTT1030840.9533752131323172No Hit
TGAGGTAGTAGGTTGTATAGT790140.7307631551980609No Hit
TGAGGTAGTAGATTGTATAGTT788420.729172408460849No Hit
TCCTGTACTGAGCTGCCCCGAGT772310.7142730305907997No Hit
TGAGGTAGTAGTTTGTGCTGTT746750.6906337941936265No Hit
TGAGGTAGTAGGTTGTGTGGTT672310.6217877551714992No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG660210.6105970368457638No Hit
TAGCTTATCAGACTGATGTTGA630770.5833693717623217No Hit
TGAGGTAGTAGGTTGTGTGGT551380.509945311606939No Hit
TCAGTGCACTACAGAACTTTGC543020.5022135425818856No Hit
CGCGACCTCAGATCAGACGT538880.4983846521795265No Hit
TGAGGTAGTAGATTGTATAGT519480.48044250874818223No Hit
TCCTGTACTGAGCTGCCCCGAGA504520.4666067115454548No Hit
CATTGCACTTGTCTCGGTCTGA495400.45817205442721465No Hit
TCGTACCGTGAGTAATAATGCG480210.44412354109102287No Hit
TCCTGTACTGAGCTGCCCCG450500.4166461657639487No Hit
TGGCTCAGTTCAGCAGGAAC430750.39838032386863687No Hit
TATTGCACTTGTCCCGGCCTGC421210.3895572285936356No Hit
TTCAAGTAATCCAGGATAGGCC417550.3861722675132892No Hit
TGTAAACATCCCCGACTGGAAGCT416680.3853676456171413No Hit
TGGAGTGTGACAATGGTGTTT398490.36854457401837054No Hit
CCCATAAAGTAGAAAGCACT395070.36538157759903045No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC385650.35666946465453236No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT376820.3485030148350081No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC375270.347069493066009No Hit
TGAGGTAGTAGGTTGTGTGG337600.31223028981555845No Hit
TAGCTTATCAGACTGATGTTG329980.3051829118286078No Hit
CGCGACCTCAGATCAGA317670.29379797442449185No Hit
TGAGGTAGTAGTTTGTACAGT313470.2899135928568813No Hit
AAAAGCTGGGTTGAGAGGGCGA310710.28736099925530856No Hit
TGAGGTAGTAGTTTGTGCTGT293980.2718882126776596No Hit
TGAGGGGCAGAGAGCGAGACTT279890.2588570373710802No Hit
TGTAAACATCCCCGACTGGAAG259280.23979582210716233No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC252170.23322011902485004No Hit
CTAGACTGAAGCTCCTTGAGG242150.22395309442783612No Hit
AAGCTGCCAGTTGAAGAACTGC239840.2218166845656503No Hit
TTCACAGTGGCTAAGTTCCG233590.216036354851944No Hit
TGTAAACATCCTTGACTGGAAGCT229020.21180977776528198No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT222280.20557627020202116No Hit
TGAGGGGCAGAGAGCGAGACC214970.19881559656887027No Hit
TAGCTTATCAGACTGATGTTGAT207950.1923231302344354No Hit
AAACCGTTACCATTACTGAG207610.19200868029800974No Hit
AAGCTGCCAGTTGAAGAACT202840.18759713266050912No Hit
TGAGGTAGTAGGTTGTATAGTC197780.1829173777242925No Hit
TGAGGGGCAGAGAGCGAGACTTC197570.182723158645912No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC194680.1800503341862942No Hit
ACCAATATTACTGTGCTGCTT187890.17377058398532372No Hit
TCCTGTACTGAGCTGCCCCGAA183470.1696827348117906No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT182410.16870239089234604No Hit
AGCTCGGTCTGAGGCCCCTCAGT176860.16356945810657486No Hit
CATTGCACTTGTCTCGGTCTG175950.16272784210025923No Hit
GCATTGGTGGTTCAGTGGTAGAATT170560.15774288575515893No Hit
AAAAGCTGGGTTGAGAGGGCGT169120.156411097789121No Hit
TGAGGTAGTAGTTTGTACAGTT168150.15551399061755378No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT166350.15384925566000637No Hit
TACAGTACTGTGATAACTGAAG165140.15273018382743284No Hit
TGTAAACATCCCCGACTGGAAGC164020.15169434874273666No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG162990.1507417504059179No Hit
TCAGTGCATCACAGAACTTTGT162050.14987238881697645No Hit
TGAGAACTGAATTCCATGGGTT152880.14139148906102658No Hit
CGCGACCTCAGATC148980.13778456331967387No Hit
TATTGCACTTGTCCCGGCCTG148290.1371464149192807No Hit
TGAGGTAGTAGTTTGTGCTGTC148170.13703543258877754No Hit
TGAGGTAGTAGATTGTATAGTC146250.13525971530072697No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCA146090.1351117388600561No Hit
TCCTGTACTGAGCTGCCCCGAGC145550.13461231837279186No Hit
AAACCGTTACCATTACTG144630.1337614538389343No Hit
AAATGGATTTTTGGAGCAGGGAGA144460.1336042288707215No Hit
TGAGGTAGTAGGTTGTGTGGTC142560.13184700863775478No Hit
TAGCTTATCAGACTGATGTTGACT142200.1315140616462453No Hit
TGAGGTAGTAGGTTGTATAGC140190.12965510761031737No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC138450.12804586381802155No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC137780.1274262124727122No Hit
AGGAAACTCTGGTG136020.12579847162533253No Hit
TTCAAGTAATCCAGGATAGGCA135050.1249013644537653No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC133880.1238192867313595No Hit
GGAATACCGGGTGCTGTAGGCTT132790.12281119722928913No Hit
AAACCGTTACCATTACTGAGTT132010.12208981208101859No Hit
ACCAATATTACTGTGCTGCTTT123030.11378463434836539No Hit
GGCTGGTCCGATGGTAGTGGGTT122810.11358116674244294No Hit
TGGCTCAGTTCAGCAGGAACAG122580.11336845060897853No Hit
AAACCGTTACCATTACTGAGA120550.11149099951796675No Hit
AACCCGTAGATCCGATCTTGT117730.10888291475114246No Hit
AAAAGCTGGGTTGAGAGGGCG117020.10822626929566544No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT115710.1070147121876726No Hit
TACAGTACTGTGATAACTGA115520.10683899016437594No Hit
TACAGTATAGATGATGTACT114680.1060621138508538No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG113630.10509101845895116No Hit
TGAGGTAGTAGGTTGTGTGGC113150.1046470891369385No Hit
TGAGGTAGTAGGTTGTATAG112870.10438813036576446No Hit
TTCAAGTAATTCAGGATAGGT112380.10393495251620989No Hit
TACCCTGTAGAACCGAATTTGT111650.103259810005649No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT108600.10043900910536034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)