FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11564963
Sequences flagged as poor quality0
Sequence length14-50
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6899855.966166947529361No Hit
CGCGACCTCAGATCAGA3570053.086953239712051No Hit
TCCTGTACTGAGCTGCCCCGA2316552.0030760150291878No Hit
TAGCTTATCAGACTGATGTTGAC2224871.9238020908497502No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2224531.923508099420638No Hit
TTCAAGTAATCCAGGATAGGCT2000631.7299060965434994No Hit
TCCTGTACTGAGCTGCCCCGAG1702831.4724041918681452No Hit
TATTGCACTTGTCCCGGCCTGT1542051.3333808331250174No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1368661.1834538510845214No Hit
TCGTACCGTGAGTAATAATGCG1038070.8975990671133145No Hit
TGAGGTAGTAGGTTGTATAGTT873170.7550132239938856No Hit
TGAGGGGCAGAGAGCGAGACTTT780180.6746065681316923No Hit
TCAGTGCACTACAGAACTTTGT777500.6722892239257489No Hit
AAACCGTTACCATTACTGAGC717790.620659140889599No Hit
TGAGGTAGTAGATTGTATAGTT708790.6128770148248637No Hit
TAGCTTATCAGACTGATGTTGA679230.5873170541055773No Hit
AAGCTGCCAGTTGAAGAACTGT664870.5749002396289551No Hit
TCCTGTACTGAGCTGCCCCGAGA631150.5457432073064133No Hit
TCCTGTACTGAGCTGCCCCG536900.46424705379515696No Hit
TGAGGTAGTAGTTTGTGCTGTT507660.4389637908915057No Hit
TGGAGTGTGACAATGGTGTTT489760.4234860068294209No Hit
TGAGGGGCAGAGAGCGAGACT480780.41572117437816275No Hit
TGAGGTAGTAGGTTGTGTGGTT479690.4147786724436559No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG475260.4109481370584584No Hit
TGAGGTAGTAGGTTGTATAGT473050.4090371927692289No Hit
TAGCTTATCAGACTGATGTTG441260.3815489941472359No Hit
TCCTGTACTGAGCTGCCCCGAGT433030.3744326722013724No Hit
TGAGGTAGTAGGTTGTGTGGT404970.35016973249287525No Hit
GGTGGGCGTAGCTC404350.34963363047508234No Hit
TGTAAACATCCCCGACTGGAAGCT359980.31126774897593706No Hit
CTAGACTGAAGCTCCTTGAGG348760.3015660318152336No Hit
TATTGCACTTGTCCCGGCCTG326600.2824047080825075No Hit
TGAGGTAGTAGGTTGTGTGG324520.28060617228087975No Hit
TGAGGTAGTAGATTGTATAGT312120.2698841319250222No Hit
TGGCTCAGTTCAGCAGGAAC305110.2638227204012672No Hit
TGTAAACATCCCCGACTGGAAGC286320.24757537053944748No Hit
AAACCGTTACCATTACTGAG286220.24748890247206154No Hit
AAAAGCTGGGTTGAGAGGGCGA274580.23742401942833713No Hit
CGCGACCTCAGATCAGAC269090.23267692252884856No Hit
TTCAAGTAATCCAGGATAGGCC251620.2175709511565234No Hit
AAACCGTTACCATTACTG242370.20957265492332314No Hit
CATTGCACTTGTCTCGGTCTGA233040.20150518423621416No Hit
TGAGGTAGTAGTTTGTACAGT232200.20077885247017221No Hit
TGGCTCAGTTCAGCAGGAACAG230230.199075431542669No Hit
CCCATAAAGTAGAAAGCACT221950.1919158755631125No Hit
TGAGGTAGTAGGTTGTATAG213810.1848773748778963No Hit
CGCGACCTCAGATCAGACGT198990.17206280729129875No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC197540.17080902031420248No Hit
CGCGACCTCAGATC196980.17032479913684115No Hit
TGTAAACATCCCCGACTGGAAG195510.16905371854626774No Hit
TACCCTGTAGAACCGAATTTGT192630.16656343820555242No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCGCAC185530.16042420542115007No Hit
TACAGTACTGTGATAACTGAAG174410.15080895632783262No Hit
CAACGGAATCCCAAAAGCAGCTG174210.15063602019306072No Hit
TATTGCACTTGTCCCGGCCTGC171700.1484656717016734No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC169960.1469611273291579No Hit
TCGTACCGTGAGTAATAATGC165110.14276742606093942No Hit
TTCACAGTGGCTAAGTTCCG162290.14032902656065566No Hit
AAGCTGCCAGTTGAAGAACT161050.1392568225250699No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC160110.138444022691642No Hit
AGCTCGGTCTGAGGCCCCTCAGT159120.13758798882452108No Hit
TGAGGGGCAGAGAGCGAGACTT151980.1314141688131644No Hit
TGAGGTAGTAGTTTGTACAGTT150440.13008256057542078No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC143550.12412491073252893No Hit
TAGCTTATCAGACTGATGTTGACA141890.12268954081392218No Hit
CTAGACTGAAGCTCCTTGAGGA141460.12231772812416261No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT138260.11955074996781226No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT135840.11745822273707231No Hit
TAGCTTATCAGACTGATGTTGACT132850.11487282752223245No Hit
CATTGCACTTGTCTCGGTCTG128330.11096447087638758No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG125420.10844825011545647No Hit
TGAGGTAGTAGTTTGTGCTGT124370.10754033540790403No Hit
TGAGAACTGAATTCCATGGGTT121160.1047647104448151No Hit
AACCCGTAGATCCGATCTTGT118980.10287970657580142No Hit
TGTAAACATCCTTGACTGGAAGCT117600.10168644724587532No Hit
GGCTGGTCCGATGGTAGTGGGTT117500.10159997917848938No Hit
ACCAATATTACTGTGCTGCTT116050.10034619220139311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)