FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002167650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8561284
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5110305.96908127332302No Hit
AAACCGTTACCATTACTGAGT3255023.8020231544707546No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2289432.6741666320145434No Hit
TCAGTGCACTACAGAACTTTGT1986802.3206799353928687No Hit
CGCGACCTCAGATCAGA1876812.1922062157966025No Hit
TTCAAGTAATCCAGGATAGGCT1452871.6970234838605984No Hit
TAGCTTATCAGACTGATGTTGAC1325341.5480621831958852No Hit
TATTGCACTTGTCCCGGCCTGT967441.130017413275859No Hit
TGGAGTGTGACAATGGTGTTT966111.1284639079838958No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC822190.9603582827061922No Hit
TGAGGTAGTAGGTTGTATAGTT779260.9102139351994397No Hit
TCCTGTACTGAGCTGCCCCGA740650.8651155597688384No Hit
TGAGGGGCAGAGAGCGAGACTTT711470.8310318872729838No Hit
TCGTACCGTGAGTAATAATGCG667510.7796844492017786No Hit
TCCTGTACTGAGCTGCCCCGAG644700.7530412494200637No Hit
AAGCTGCCAGTTGAAGAACTGT569420.6651105137967622No Hit
TGAGGTAGTAGATTGTATAGTT554390.6475547359484862No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT526170.6145923905806652No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC516710.6035426461731674No Hit
TAGCTTATCAGACTGATGTTGA467600.5461797552796988No Hit
TGAGGTAGTAGGTTGTATAGT424360.4956733125545187No Hit
AAACCGTTACCATTACTGAGC419550.4900549964234337No Hit
TGGCTCAGTTCAGCAGGAAC369700.43182774920210565No Hit
AAAAGCTGGGTTGAGAGGGCGA368890.4308816294378274No Hit
TGTAAACATCCCCGACTGGAAGCT330340.3858533369527281No Hit
TGAGGTAGTAGGTTGTGTGGTT326070.380865767331162No Hit
TTCACAGTGGCTAAGTTCCG320020.3737990703263669No Hit
TGAGGTAGTAGTTTGTGCTGTT317710.3711008769245361No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC306170.3576215904062989No Hit
TGAGGGGCAGAGAGCGAGACT276200.32261515912799993No Hit
TCAGTGCACTACAGAACTTTGC272930.3187956385981355No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT264170.30856352855482894No Hit
TAGCTTATCAGACTGATGTTG263660.30796782351806107No Hit
CCCATAAAGTAGAAAGCACT253880.2965443034012188No Hit
TGAGGTAGTAGATTGTATAGT252920.29542297627318514No Hit
TCCTGTACTGAGCTGCCCCGAGA252500.2949323956546705No Hit
CGCGACCTCAGATC248200.2899097845603533No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT244780.2859150566667336No Hit
TGTAAACATCCCCGACTGGAAGC244540.28563472488472524No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC239260.27946742568054045No Hit
CTAGACTGAAGCTCCTTGAGG236030.27569462711434406No Hit
TCCTGTACTGAGCTGCCCCGAGT230730.26950396692832523No Hit
TGAGAACTGAATTCCATGGGTT228310.26667728812640723No Hit
TTCAAGTAATCCAGGATAGGCC226390.26443463387034No Hit
TGAGGTAGTAGGTTGTGTGGT226030.26401413619732744No Hit
TGGCTCAGTTCAGCAGGAACAG222720.2601478937037949No Hit
TCCTGTACTGAGCTGCCCCG214470.25051148869725615No Hit
CGCGACCTCAGATCAGACGT209230.24439091145673944No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT199270.23275714250339083No Hit
TGTAAACATCCCCGACTGGAAG184070.2150027963095255No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT182730.2134376105266453No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA175970.20554159866674207No Hit
TGAGGGGCAGAGAGCGAGACTT173660.2028434052649112No Hit
AGCTCGGTCTGAGGCCCCTCAGT173480.20263315642840488No Hit
TGAGGTAGTAGGTTGTGTGG172100.20102124868185658No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA165420.1932186807492895No Hit
CATTGCACTTGTCTCGGTCTGA165140.192891627003613No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC164480.1921207146030899No Hit
AAACCGTTACCATTACTGAG163490.19096434600230525No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT163180.19060225078387774No Hit
TGAGGTAGTAGGTTGTATAG161540.188686650273487No Hit
TGAGGTAGTAGTTTGTACAGT159920.18679441074493033No Hit
CAACGGAATCCCAAAAGCAGCTG159450.18624542767183053No Hit
GGCTGGTCCGATGGTAGTGGGTTATC151800.1773098521203128No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA151120.17651557873795565No Hit
TATTGCACTTGTCCCGGCCTG150500.17579138830110064No Hit
GGCTGGTCCGATGGTAGTGGGTT139580.16303629221971844No Hit
TATTGCACTTGTCCCGGCCTGC139440.1628727653468802No Hit
CGCGACCTCAGATCAGAC139150.16253403111028672No Hit
AAATGGATTTTTGGAGCAGGGAGA134330.1569040344882847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT133430.15585279030575322No Hit
AAACCGTTACCATTACTG126070.14725594899082894No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG125990.14716250506349282No Hit
GGCTGGTCCGATGGTAGTG124950.14594773400812308No Hit
TGGAGTGTGACAATGGTGTTTG114720.13399859180001505No Hit
TCGTACCGTGAGTAATAATGC111630.1303893201066569No Hit
TGAGATGAAGCACTGTAGCTC110320.1288591757965277No Hit
AACCCGTAGATCCGATCTTGT109070.12739911443190063No Hit
AAATGGATTTTTGGAGCAGGGA108460.1266866044859626No Hit
TGAGATGAAGCACTGTAGCT107480.125541916376095No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC106170.12401177206596581No Hit
TGAGGTAGTAGTTTGTACAGTT105930.1237314402839574No Hit
CTAGACTGAAGCTCCTTGAGGA103970.12144206406422214No Hit
AAATGGATTTTTGGAAATAGGAGA103000.12030905644527153No Hit
AGAAGCCGGCGGGAGC102330.11952646355383141No Hit
TGTAAACATCCTTGACTGGAAGCT101120.11811312415287238No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT100280.11713196291584299No Hit
GGCTGGTCCGATGGTAGT99270.11595223333322431No Hit
TAGCTTATCAGACTGATGTTGACT98360.1148893086597758No Hit
TAGCTTATCAGACTGATGTTGACA98280.11479586473243968No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC97130.11345260827698275No Hit
CAACGGAATCCCAAAAGCAGCT96930.11321899845864242No Hit
AAATGGATTTTTGGAGCAGGG96130.11228455918528109No Hit
CGTGTATTTGACAAGCTGAGTTG95100.11108146862082836No Hit
AAAAGCTGGGTTGAGAGGGCG93830.10959804627436724No Hit
GTTTCCGTAGTGTAGTGGTTATC92390.10791605558231686No Hit
TTCAAGTAATTCAGGATAGGT91600.10699329679987254No Hit
TACAGTACTGTGATAACTGAAG90690.10593037212642403No Hit
TGAGGTAGTAGGTTGTATAGTC88690.10359427394302069No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA88540.10341906657926545No Hit
AGAGGTAGTAGGTTGCATAGTT88110.10291680546983373No Hit
AAATGGATTTTTGGAGCAGGGAG87680.10241454436040202No Hit
GGCTGGTCCGATGGTAGTGGGT86350.1008610390684388No Hit
TAGCTTATCAGACTGATGTTGAT86240.10073255366835161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)