FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13845849
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8162095.8949725654237595No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4358533.1478965284107896No Hit
TCAGTGCACTACAGAACTTTGT3573452.5808818224147902No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3073962.220131102108654No Hit
TTCAAGTAATCCAGGATAGGCT2165981.5643533307347204No Hit
TGAGGTAGTAGGTTGTATAGTT1557611.1249653235420956No Hit
TATTGCACTTGTCCCGGCCTGT1511451.0916268117614167No Hit
TCCTGTACTGAGCTGCCCCGAG1465171.0582016314059182No Hit
TGAGGTAGTAGGTTGTATAGT1460081.054525439357312No Hit
TGAGGTAGTAGATTGTATAGTT1275630.9213086174780614No Hit
AAACCGTTACCATTACTGAGC1252280.9044443572943774No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1138190.8220442097844631No Hit
CGCGACCTCAGATCAGA1128490.8150384999865302No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1030600.7443386100772874No Hit
TGAGGTAGTAGATTGTATAGT1019010.7359678702259428No Hit
CGCGACCTCAGATCAGACGT950430.6864367797164335No Hit
TAGCTTATCAGACTGATGTTGAC922690.6664018941705923No Hit
TCCTGTACTGAGCTGCCCCGA861580.6222659224436147No Hit
AAGCTGCCAGTTGAAGAACTGT827990.5980059438753088No Hit
TGAGGTAGTAGTTTGTGCTGTT796340.5751471072665895No Hit
TGAGGGGCAGAGAGCGAGACTTT749670.5414402540429265No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT730530.5276166163591701No Hit
TGAGGTAGTAGTTTGTACAGT723990.5228931790314917No Hit
TGAGGTAGTAGGTTGTGTGGTT716210.5172741664306754No Hit
CGCGACCTCAGATC710880.5134246372324297No Hit
TCCTGTACTGAGCTGCCCCGAGT626530.4525038515153531No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC621390.4487915475605721No Hit
TGAGGTAGTAGGTTGTGTGGT587300.42417044993051706No Hit
TCAGTGCACTACAGAACTTTGC586190.423368765613434No Hit
TCCTGTACTGAGCTGCCCCGAGA581860.42024147453868665No Hit
TAGCTTATCAGACTGATGTTGA563620.4070678511660787No Hit
CCCATAAAGTAGAAAGCACT559010.40373833341675186No Hit
TGAGGTAGTAGTTTGTACAGTT558360.40326887863647803No Hit
CATTGCACTTGTCTCGGTCTGA556220.40172328905219173No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC500770.36167518510421426No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT499940.36107572746171074No Hit
AAAAGCTGGGTTGAGAGGGCGA434100.3135235694105865No Hit
TTCAAGTAATCCAGGATAGGCC430900.3112124074153922No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC415510.3000971626947542No Hit
TGTAAACATCCCCGACTGGAAGCT414230.29917269789667644No Hit
AAATGGATTTTTGGAAATAGGAGA412890.2982048988111888No Hit
AAACCGTTACCATTACTGAG407120.294037584838604No Hit
TGAGAACTGAATTCCATGGGTT406860.29384980292649443No Hit
TCGTACCGTGAGTAATAATGCG396340.28625185786729296No Hit
TGGCTCAGTTCAGCAGGAAC388590.2806545124101816No Hit
TTCAAGTAATTCAGGATAGGT363130.26226632978591635No Hit
TTCACAGTGGCTAAGTTCCG358870.25918959537981384No Hit
AAATGGATTTTTGGAGCAGGGAGA344890.2490927064133084No Hit
AAATGGATTTTTGGAGCAGGGA339010.24484594624713876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT336820.24326424475667763No Hit
AAACCGTTACCATTACTGAGTT335700.2424553380583596No Hit
TGAGGGGCAGAGAGCGAGACT333400.2407941903743136No Hit
TGAGGTAGTAGGTTGTATAG327290.2363813154397394No Hit
TATTGCACTTGTCCCGGCCTGC314510.22715111222143183No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT306910.2216621024828452No Hit
TGGAGAGAAAGGCAGTTCCTGA297420.21480806269084693No Hit
AAATGGATTTTTGGAGCAGGG281820.20354114796427436No Hit
AAATGGATTTTTGGAAATAGGA272890.19709156152143506No Hit
TCCTGTACTGAGCTGCCCCG268340.19380537805951806No Hit
TGAGGTAGTAGTTTGTGCTGT261200.18864859785774063No Hit
AAATGGATTTTTGGAAATAGGAG256840.18549963963928828No Hit
AAATGGATTTTTGGAGCAGGGAGAT256680.18538408153952857No Hit
CAACGGAATCCCAAAAGCAGCT256070.18494351628419461No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT248880.17975062417624227No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT243940.17618276784616096No Hit
TGAGGTAGTAGGTTGTATAGTC243820.17609609927134118No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT243190.17564108925353727No Hit
TGAGGGGCAGAGAGCGAGACTT241910.17471662445545955No Hit
AGAGGTAGTAGGTTGCATAGTT240470.17367660155762207No Hit
TGTAAACATCCCCGACTGGAAG237210.17132210527501782No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT237020.17118488003155316No Hit
AGCTCGGTCTGAGGCCCCTCAGT232540.16794925323828103No Hit
TGTAAACATCCTTGACTGGAAGCT230160.1662303265043552No Hit
TGAGGTAGTAGGTTGTATAGC228250.16485085168847358No Hit
GCATTGGTGGTTCAGTGGTAGAATT227990.16466306977636402No Hit
TAGCTTATCAGACTGATGTTG221320.15984574149263076No Hit
GGCTGGTCCGATGGTAGT220580.15931128528124205No Hit
AAATGGATTTTTGGAAATAGGAGAT217680.15721679472309716No Hit
AGAGGTAGTAGGTTGCATAGT215910.15593843324450526No Hit
TGAGATGAAGCACTGTAGCT211550.15278947502605295No Hit
AAATGGATTTTTGGAGCAGGGAG207940.1501821954002243No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA206950.14946717965796102No Hit
CAACGGAATCCCAAAAGCAGCTG202520.14626766477086384No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG199110.14380483276973482No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC197870.142909257496597No Hit
TGAGGTAGTAGATTGTATAGTC193200.1395364054598602No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT192740.139204175923051No Hit
TGAGGTAGTAGATTGTATAG184150.13300015044220112No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG183470.13250902851822233No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA183140.1322706899374679No Hit
GTTTCCGTAGTGTAGTGGTTATC181750.13126677894580535No Hit
GGCTGGTCCGATGGTAGTGGGT179620.1297284117427541No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC175650.12686112639246608No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC174820.12626166874996253No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA172180.12435496010392717No Hit
CGCGACCTCAGATCAGACGC171060.12354605340560915No Hit
TTCAAGTAATTCAGGATAGGTT168650.12180545952797839No Hit
CATTGCACTTGTCTCGGTCTG165680.11966041230118861No Hit
AAACCGTTACCATTACTG162390.1172842488748794No Hit
TGGCTCAGTTCAGCAGGAACAG160490.11591199644023274No Hit
AAATGGATTTTTGGAAATAGGAGATGGAAT159750.11537754022884403No Hit
CTAGACTGAAGCTCCTTGAGG154140.1113257843560189No Hit
TGAGGTAGTAGATTGTATAGC153870.11113078006267439No Hit
TGTAAACATCCCCGACTGGAAGC153660.11097911005673974No Hit
CGCGACCTCAGATCAGACG152400.11006909002113197No Hit
AAAAGCTGGGTTGAGAGGGCGAT150900.1089857328358846No Hit
AGTTGGTCCGAGTGTTGTGGGT148580.10731014038936869No Hit
GGCTGGTCCGATGGTAGTGGGTT148470.10723069419578389No Hit
TACAGTACTGTGATAACTGAAG146920.10611122510436162No Hit
GGCTGGTCCGATGGTAGTG146890.10608955796065665No Hit
TGAGGTAGTAGGTTGTAT145040.10475341743218491No Hit
GGCTGGTCCGATGGTAGTGGGTTATC144540.10439229837043579No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCC143600.10371339453434744No Hit
TTCAAGTAATCCAGGATAGGCA142870.10318616070419373No Hit
AAGCTGCCAGTTGAAGAACTGC142240.10273115068638984No Hit
AGCAGCATTGTACAGGGCTATGA140400.10140223253915306No Hit
TACAGTATAGATGATGTACT138580.10008775915438628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)