FastQCFastQC Report
Wed 8 May 2024
EGAF00002167652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11540432
Total Bases268 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5581554.836517385137749No Hit
AAACCGTTACCATTACTGAGT5043124.369957727752306No Hit
TCAGTGCACTACAGAACTTTGT2863752.4814928938535403No Hit
CGCGACCTCAGATCAGA2451712.12445253349268No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2035301.7636254864635916No Hit
TCCTGTACTGAGCTGCCCCGAG1560201.3519424576133718No Hit
TTCAAGTAATCCAGGATAGGCT1344801.16529433213592No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1232351.0678543056273804No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1211921.050151328823739No Hit
TATTGCACTTGTCCCGGCCTGT1144500.9917306388530343No Hit
TCCTGTACTGAGCTGCCCCGA985650.8540841452035766No Hit
TGAGGTAGTAGGTTGTATAGTT976270.8459561999065547No Hit
CGCGACCTCAGATC872650.7561675334164267No Hit
TAGCTTATCAGACTGATGTTGAC872130.7557169436984681No Hit
TGAGGTAGTAGATTGTATAGTT755400.6545682172036541No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT739090.640435297396146No Hit
AAGCTGCCAGTTGAAGAACTGT642770.5569722173312056No Hit
TTCACAGTGGCTAAGTTCCG608960.5276752204770151No Hit
TGGCTCAGTTCAGCAGGAAC597370.5176322688786693No Hit
TGAGGGGCAGAGAGCGAGACTTT590230.5114453254436229No Hit
TCCTGTACTGAGCTGCCCCGAGA582280.5045565018709871No Hit
TCGTACCGTGAGTAATAATGCG580900.5033607060810202No Hit
AAAAGCTGGGTTGAGAGGGCGA574810.4980836072687747No Hit
TAGCTTATCAGACTGATGTTGA556280.48202701597305886No Hit
TGAGGTAGTAGGTTGTATAGT550030.47661127417067234No Hit
CGCGACCTCAGATCAGACGT546390.4734571461449623No Hit
CATTGCACTTGTCTCGGTCTGA521000.451456236646947No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT467490.4050888216316339No Hit
AAACCGTTACCATTACTGAGC454970.394240007653093No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC423930.3673432675657202No Hit
TGAGGTAGTAGGTTGTGTGGTT423010.36654607037240894No Hit
CCCATAAAGTAGAAAGCACT405620.35147731038144847No Hit
AAACCGTTACCATTACTGAG400600.3471273865657716No Hit
TGAGGTAGTAGGTTGTATAG395220.34246551602227715No Hit
TGAGGTAGTAGTTTGTACAGT366050.3171891658821784No Hit
TGAGGTAGTAGATTGTATAGT360810.3126486079550575No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC358030.31023968600135593No Hit
TGGCTCAGTTCAGCAGGAACAG353260.3061063918577745No Hit
TCCTGTACTGAGCTGCCCCGAGT349950.30323821499923054No Hit
TGAGGTAGTAGTTTGTGCTGTT346700.3004220292619895No Hit
TGTAAACATCCCCGACTGGAAG345410.29930422015397695No Hit
AAATGGATTTTTGGAAATAGGAGA337560.29250204845017935No Hit
TGAGAACTGAATTCCATGGGTT322590.2795302636851029No Hit
TCCTGTACTGAGCTGCCCCG314240.27229483263711446No Hit
TGAGGTAGTAGGTTGTGTGGT307480.2664371663036531No Hit
TAGCTTATCAGACTGATGTTG300640.2605101784751212No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA294470.2551637581678051No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA280740.2432664565763223No Hit
TGAGGTAGTAGTTTGTACAGTT280520.24307582246487824No Hit
TGGAGAGAAAGGCAGTTCCTGA273070.23662025823643343No Hit
TCAGTGCACTACAGAACTTTGC272500.2361263425840558No Hit
TGTAAACATCCCCGACTGGAAGCT269120.23319750941732512No Hit
TGGAGTGTGACAATGGTGTTT269050.2331368531091384No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC252280.21860533470497465No Hit
GGCTGGTCCGATGGTAGTG249280.21600577863982906No Hit
AAATGGATTTTTGGAGCAGGGAGA240490.20838907936895257No Hit
CTAGACTGAAGCTCCTTGAGG235600.20415180298276528No Hit
TGAGGGGCAGAGAGCGAGACT232010.20104100089147442No Hit
CAACGGAATCCCAAAAGCAGCTG222270.19260110886663515No Hit
TGTAAACATCCCCGACTGGAAGC218940.18971560163432358No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT212320.1839792479172357No Hit
CGCGACCTCAGATCAGACG201990.1750281098662511No Hit
AAACCGTTACCATTACTG199470.17284448277152883No Hit
GGCTGGTCCGATGGTAGTGGGTTATC194960.16893648348692666No Hit
GCATTGGTGGTTCAGTGGTAGAATT194290.1683559159657108No Hit
CGTGTATTTGACAAGCTGAGTTG192890.16714278980197622No Hit
GGCTGGTCCGATGGTAGT191730.16613762812345326No Hit
TGAGATGAAGCACTGTAGCT190980.16548773910716685No Hit
TGAAAAGAACTTTGAAGAGAG190040.16467321154008793No Hit
AGGAAACTCTGGTG188650.16346875056323715No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC188510.16334743794686368No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT187950.16286218748136985No Hit
TGAGGTAGTAGATTGTATAG186840.161900351737266No Hit
TTCAAGTAATTCAGGATAGGT182060.15775839240680073No Hit
TGAGGGGCAGAGAGCGAGACTT181700.15744644567898325No Hit
CGCGACCTCAGATCAGAC181670.1574204501183318No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG181560.15732513306260978No Hit
GTTTCCGTAGTGTAGTGGTTATC179040.1551415059678875No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC177350.15367708938452218No Hit
AGCTCGGTCTGAGGCCCCTCAGT175650.15220400761427302No Hit
TGAGGTAGTAGGTTGTGTGG172720.14966510785731418No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA170130.14742082445440519No Hit
AAATGGATTTTTGGAAATAGGAG168360.14588708637596928No Hit
TGAGATGAAGCACTGTAGCTC168120.14567912189075763No Hit
CATTGCACTTGTCTCGGTCTG167030.1447346165204214No Hit
TGGCTCAGTTCAGCAGGAACA156710.13579214365632067No Hit
AAATGGATTTTTGGAGCAGGGA155180.13446637006309645No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT154780.1341197625877437No Hit
GGCTGGTCCGATGGTAGTGGGT154050.13348720394522492No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT151650.13140755909310847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG151130.13095696937514992No Hit
AGAAGCCGGCGGGAGC149970.12995180769662695No Hit
TGAGGTAGTAGTTTGTGCTGT149860.12985649064090496No Hit
TTCAAGTAATCCAGGATAGGCC148300.12850472148702924No Hit
TATTGCACTTGTCCCGGCCTG147140.1274995598085063No Hit
TGTAAACATCCTTGACTGGAAGCT146600.1270316397167801No Hit
AGAGGTAGTAGGTTGCATAGTT146010.1265203936906348No Hit
GGCTGGTCCGATGGTAGTGGGTT142700.12365221683209085No Hit
AAACCGTTACCATTACTGAGTT139980.12129528599969222No Hit
TACAGTACTGTGATAACTGAAG138280.11982220422944306No Hit
AAAAGCTGGGTTGAGAGGGCG135170.11712733110857548No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA132490.11480506102371212No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC131960.11434580611886973No Hit
TGTCAGTTTGTCAAATACCCCAA130170.1127947376666662No Hit
AGCAGCATTGTACAGGGCTATGA129930.11258677318145456No Hit
AAAAGCTGGGTTGAGAGGGCGAA129300.11204086640777398No Hit
TAGCAGCACGTAAATATTGGCG128390.1112523344013465No Hit
TGTAAACATCCCCGACTGGAA127160.11018651641463682No Hit
AAATGGATTTTTGGAGCAGGGAG125960.10914669398857858No Hit
AAAAGCTGGGTTGAGAGGGCGAT124780.10812420193628801No Hit
AAATGGATTTTTGGAAATAGGA123860.1073270047429767No Hit
CAACGGAATCCCAAAAGCAGCT122560.10620053044808027No Hit
AAATGGATTTTTGGAGCAGGG118520.10269979494701759No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG117240.10159065102588881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)