FastQCFastQC Report
Wed 8 May 2024
EGAF00002167653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12878733
Total Bases284.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT161806012.563813536626622No Hit
TCAGTGCACTACAGAACTTTGT5693444.4208075437234395No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2560561.9882080015169197No Hit
AAACCGTTACCATTACTGAGC2398901.8626832313396045No Hit
TGGAGTGTGACAATGGTGTTT2364971.8363374720168513No Hit
TGAGGTAGTAGGTTGTATAGT2120831.6467691348209486No Hit
TCCTGTACTGAGCTGCCCCGAG1966581.5269980362198672No Hit
TATTGCACTTGTCCCGGCCTGT1841931.4302105649678427No Hit
TTCAAGTAATCCAGGATAGGCT1831201.4218790000538095No Hit
TGAGGTAGTAGGTTGTGTGGT1540091.1958396839192178No Hit
TGAGGTAGTAGGTTGTATAGTT1523911.1832763362669294No Hit
TCCTGTACTGAGCTGCCCCGA1407461.092855950969711No Hit
AAGCTGCCAGTTGAAGAACTGT1400461.087420633691218No Hit
CGCGACCTCAGATCAGA1387501.0773575319870363No Hit
TGAGGTAGTAGATTGTATAGT1235530.9593567938709499No Hit
TGAGGTAGTAGGTTGTGTGGTT1190980.9247648817628256No Hit
TGAGGGGCAGAGAGCGAGACTTT1173040.9108349400519445No Hit
TGAGGTAGTAGATTGTATAGTT1033020.8021130650041428No Hit
TGAGGGGCAGAGAGCGAGACT1021990.7935485579210316No Hit
TCAGTGCACTACAGAACTTTGC882880.685533274119434No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT846320.6571453884477612No Hit
CATTGCACTTGTCTCGGTCTGA795590.6177548676566242No Hit
TGAGGTAGTAGTTTGTGCTGTT745570.578915643332306No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT726610.564193698246559No Hit
TGAGGTAGTAGTTTGTACAGT716890.5566463719684227No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC710310.5515371737266391No Hit
CCCATAAAGTAGAAAGCACT678890.5271403638851742No Hit
TCCTGTACTGAGCTGCCCCGAGA636570.4942799885671983No Hit
TAGCTTATCAGACTGATGTTGAC611120.47451872789039107No Hit
AAACCGTTACCATTACTGAG607250.47151377390928134No Hit
CGCGACCTCAGATCAGACGT582380.45220286809269206No Hit
TGGCTCAGTTCAGCAGGAAC578820.44943862101962984No Hit
TAGCTTATCAGACTGATGTTGA545440.42351992234018676No Hit
TTCACAGTGGCTAAGTTCCG525730.40821562183174387No Hit
TCCTGTACTGAGCTGCCCCGAGT524820.4075090305855397No Hit
AAAAGCTGGGTTGAGAGGGCGA512700.3980981669547773No Hit
TGAGGTAGTAGGTTGTATAG432610.33591037254984635No Hit
TCGTACCGTGAGTAATAATGCG430830.33452824901331524No Hit
TCCTGTACTGAGCTGCCCCG396020.3074991926612657No Hit
TGAGGTAGTAGTTTGTACAGTT388780.30187752164750986No Hit
TGAGGTAGTAGGTTGTGTGG387460.30085257610356547No Hit
TATTGCACTTGTCCCGGCCTGC356120.2765178841738547No Hit
AAACCGTTACCATTACTGAGTT354940.2756016449754801No Hit
TTCAAGTAATCCAGGATAGGCC344130.26720796214969283No Hit
TTCAAGTAATTCAGGATAGGT326800.25375166951593764No Hit
TGAGATGAAGCACTGTAGCT325240.2525403702367306No Hit
TGGCTCAGTTCAGCAGGAACAG324590.25203566220372764No Hit
TGGAGTGTGACAATGGTGTTC321590.24970624051294488No Hit
TGAGGTAGTAGGTTGTATAGC318850.24757870203536325No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC313540.24345562564267775No Hit
TGAGAACTGAATTCCATGGGTT311200.24163867672386719No Hit
TGGAGTGTGACAATGGTGTTTG290640.22567437340303584No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT288430.22395836609082587No Hit
TAGCTTATCAGACTGATGTTG283300.2199750549995873No Hit
TGAGGTAGTAGGTTGTGTGGC262960.20418157593608005No Hit
TGAGGGGCAGAGAGCGAGACTT260810.20251215705768574No Hit
CATTGCACTTGTCTCGGTCTG243640.18918010024743895No Hit
CTAGACTGAAGCTCCTTGAGG243090.18875303960412876No Hit
TGAGGTAGTAGGTTGTATAGTC237680.18455231582175047No Hit
TGTAAACATCCTTGACTGGAAGCT234010.18170265662002621No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA232770.180739828987836No Hit
TGTAAACATCCCCGACTGGAAGCT232180.1802817093886487No Hit
TGAGGTAGTAGTTTGTGCTGT229650.17831723042942188No Hit
AAGCTGCCAGTTGAAGAACTGC228270.17724569645166183No Hit
AAACCGTTACCATTACTG225880.17538992383800486No Hit
AGGAAACTCTGGTG221470.1719656739525542No Hit
AGCTCGGTCTGAGGCCCCTCAGT215400.1672524773982037No Hit
CGTGTATTTGACAAGCTGAGTTG211070.1638903454245072No Hit
TGTAAACATCCCCGACTGGAAG209520.16268681088426945No Hit
AAGCTGCCAGTTGAAGAACT208650.16201127859394243No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA207520.16113386309041425No Hit
TGAGGTAGTAGGTTGTGTGGTC207450.16107950991762932No Hit
CGCGACCTCAGATC206580.1604039776273023No Hit
TCCTGTACTGAGCTGCCCCGAA201820.15670796187792696No Hit
TGGCTCAGTTCAGCAGGAACA194280.1508533486950929No Hit
AGAGGTAGTAGGTTGCATAGT193310.15010016901507314No Hit
TGGAGTGTGACAATGGTGTTTGT189620.14723498033541033No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT188890.14666815439065317No Hit
TGAGGTAGTAGATTGTATAGC184210.14303425655303206No Hit
AAACCGTTACCATTACTGAGA182390.14162107406062382No Hit
TGAGATGAAGCACTGTAGCTC181250.14073589381812637No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG178150.13832882473765082No Hit
TGAGGTAGTAGATTGTATAG174760.1356965782270663No Hit
TGAGGTAGTAGGTTGTAT174760.1356965782270663No Hit
TGGAGAGAAAGGCAGTTCCTGA171850.133437039187007No Hit
TGAGGGGCAGAGAGCGAGACC170460.13235774047027762No Hit
TCAGTGCATCACAGAACTTTGT166240.12908102062524318No Hit
AGAGGTAGTAGGTTGCATAGTT162500.126177008250734No Hit
AAAAGCTGGGTTGAGAGGGCGT161750.1255946528280383No Hit
CAACGGAATCCCAAAAGCAGCT161520.12541606383174494No Hit
TAGCAGCACGTAAATATTGGCG161340.12527629853029798No Hit
TGAGGTAGTAGATTGTATAGTC157750.12248875724032791No Hit
AAAAGCTGGGTTGAGAGGGCG156310.12137063482875218No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT151580.11769791329628465No Hit
TACAGTATAGATGATGTACT144890.11250330292583906No Hit
TGAGGTAGTAGTTTGTGCT143440.11137741577529403No Hit
TACAGTACTGTGATAACTGAAG139860.10859763922429326No Hit
TGAGGGGCAGAGAGCGAGACTTC139040.10796093062881264No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC138300.10738633994508621No Hit
AAACCGTTACTATTACTGAGT135670.10534421359616664No Hit
ACCAATATTACTGTGCTGCTT135610.105297625162351No Hit
TGAGGTAGTAGATTGTAT135170.10495597664770284No Hit
AAAAGCTGGGTTGAGAGGGCGAT134640.10454444548233122No Hit
GGCTGGTCCGATGGTAGTGGGTTATC134180.10418726748974454No Hit
TTCAAGTAATCCAGGATAGGCA133850.10393103110375843No Hit
TGAAAAGAACTTTGAAGAGAG130480.10131431407111244No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC129180.10030489800510656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)