FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12822469
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT132585810.34011468462119No Hit
TCAGTGCACTACAGAACTTTGT3042832.3730453159995943No Hit
TGGAGTGTGACAATGGTGTTT1887101.4717134430194372No Hit
TCCTGTACTGAGCTGCCCCGAG1822531.4213565265784616No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1569431.2239686444162976No Hit
AAACCGTTACCATTACTGAGC1441191.1239567044381233No Hit
TGAGGTAGTAGGTTGTATAGT1424451.110901496427872No Hit
TGAGGTAGTAGGTTGTGTGGT1366841.065972551776105No Hit
TTCAAGTAATCCAGGATAGGCT1234470.9627397032505987No Hit
CGCGACCTCAGATCAGA1117100.8712050697880416No Hit
TCCTGTACTGAGCTGCCCCGA1103530.8606220845610935No Hit
AAGCTGCCAGTTGAAGAACTGT1076340.8394171200569874No Hit
TATTGCACTTGTCCCGGCCTGT1069000.8336927934861843No Hit
TGAGGTAGTAGGTTGTGTGGTT994820.7758412205948793No Hit
TGAGGTAGTAGGTTGTATAGTT895340.6982586582973997No Hit
TGAGGTAGTAGATTGTATAGT858320.6693874635220409No Hit
TGAGGGGCAGAGAGCGAGACTTT712610.5557510024005518No Hit
AAACCGTTACCATTACTGAGG695770.5426178062898807No Hit
TGAGGGGCAGAGAGCGAGACT669940.5224734799514821No Hit
AAACCGTTACCATTACTGAG626220.4883770824480059No Hit
TGAGGTAGTAGATTGTATAGTT624880.4873320419023825No Hit
TCCTGTACTGAGCTGCCCCGAGA542400.4230074566762454No Hit
CCCATAAAGTAGAAAGCACT512590.3997592039411442No Hit
TGAGGTAGTAGTTTGTACAGT490300.3823756563576016No Hit
TTCACAGTGGCTAAGTTCCG481170.3752553427892865No Hit
TGAGGTAGTAGTTTGTGCTGTT467060.36425122182007225No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT460390.3590494155220808No Hit
TCGTACCGTGAGTAATAATGCG454650.35457289855799223No Hit
TAGCTTATCAGACTGATGTTGA446180.3479673064524469No Hit
CATTGCACTTGTCTCGGTCTGA441870.34460601932435947No Hit
TGGCTCAGTTCAGCAGGAAC422420.32943733379273527No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT418230.3261696323851514No Hit
TCCTGTACTGAGCTGCCCCGAGT417310.3254521418612905No Hit
TAGCTTATCAGACTGATGTTGAC413290.3223170202244201No Hit
TGAGGTAGTAGGTTGTGTGG409850.3196342295699838No Hit
TCCTGTACTGAGCTGCCCCG377960.2947638243461536No Hit
TGAGGTAGTAGGTTGTATAG367130.28631771307070425No Hit
TGGAGTGTGACAATGGTGTTTG355130.27695914102034486No Hit
CGCGACCTCAGATCAGACGT354060.2761246683458545No Hit
TCAGTGCACTACAGAACTTTGC348760.27199129902361235No Hit
AAAAGCTGGGTTGAGAGGGCGA319750.2493669510918685No Hit
TAGCTTATCAGACTGATGTTG299230.2333637928857539No Hit
TTCAAGTAATTCAGGATAGGT287260.22402861726552042No Hit
AAACCGTTACCATTACTG252970.19728649763161837No Hit
TGGCTCAGTTCAGCAGGAACAG231110.18023829887988033No Hit
TGTAAACATCCCCGACTGGAAGCT201640.157255205686206No Hit
TGAGGTAGTAGTTTGTACAGTT198730.15498575196399383No Hit
CTAGACTGAAGCTCCTTGAGG194650.15180383746687165No Hit
TGTAAACATCCCCGACTGGAAG189890.14809160388689574No Hit
AAGCTGCCAGTTGAAGAACT189710.14795122530614033No Hit
AAACCGTTACCATTACTGAGTT184790.14411421076549297No Hit
TGGAGTGTGACAATGGTGTTTGT180940.141111668899336No Hit
TTCAAGTAATCCAGGATAGGCC179810.14023040336459383No Hit
CATTGCACTTGTCTCGGTCTG178060.13886561160724975No Hit
TGAGGTAGTAGGTTGTGTGGC177890.13873303183653632No Hit
TCAGTGCACTACAGAACTTTGG174840.13635439477373662No Hit
TGAGAACTGAATTCCATGGGTT173880.1356057090097079No Hit
TGGAGTGTGACAATGGTGTTC173710.13547312923899446No Hit
TGAGATGAAGCACTGTAGCT171330.1336170124490065No Hit
TGTAAACATCCTTGACTGGAAGCT166210.12962402170751983No Hit
GGAATACCGGGTGCTGTAGGCTT165590.12914049548491793No Hit
GGCCTTGAGGGCTGTTGTGGAGAAG164980.128664768072358No Hit
TACAGTACTGTGATAACTGAAG161000.12556084167565545No Hit
TGAGGTAGTAGATTGTATAG160540.125202096413725No Hit
TGAGGGGCAGAGAGCGAGACTT160230.12496033330242405No Hit
TGAGGTAGTAGGTTGTATAGC156010.12166923546471431No Hit
TATTGCACTTGTCCCGGCCTGC154040.12013286988644697No Hit
CGTGTATTTGACAAGCTGAGTTG153520.11972733176426474No Hit
ACCAATATTACTGTGCTGCTT151070.11781662330398303No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA150480.11735649351150702No Hit
AGCTCGGTCTGAGGCCCCTCAGT146220.11403420043362943No Hit
TACAGTATAGATGATGTACT144620.11278639082691484No Hit
CAACGGAATCCCAAAAGCAGCT143880.11220927888380934No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA141390.11026737518335977No Hit
TGAGGTAGTAGTTTGTGCTGT137230.10702307020590185No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC136060.10611060943099179No Hit
TGGCTCAGTTCAGCAGGAACA134070.10455864623264052No Hit
AAAAGCTGGGTTGAGAGGGCG132320.10319385447529646No Hit
AAGCTGCCAGTTGAAGAACTGC128410.10014451974888768No Hit
TGGAGAGAAAGGCAGTTCCTGA128290.10005093402838407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)