FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10690350
Sequences flagged as poor quality0
Sequence length14-50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT4996834.674150051214413No Hit
CGGACAGGATTGACAGA2968202.776522751827583No Hit
TGGAGTGTGACAATGGTGTTT1555391.4549476864648958No Hit
CACGGACAGGATTGACAGA1388901.29920909979561No Hit
CCGGACACGGACAGGATTGACAGA1368151.279799071124893No Hit
TCAGTGCACTACAGAACTTTGT1255141.1740869101572915No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1067650.9987044390501715No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAC1020470.9545711786798374No Hit
TGAGGTAGTAGGTTGTATAGT938310.8777168193744825No Hit
TCAAGGCCGAGAGCTGATGACGAGTCGT824280.7710505268770433No Hit
AAACCGTTACCATTACTGAGC787560.7367017918028876No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCA776100.725981843438241No Hit
TGAGGTAGTAGGTTGTATAGTT758020.7090693943603343No Hit
ACACGGACAGGATTGACAGA713230.6671717951236396No Hit
TTCAAGTAATCCAGGATAGGCT689780.6452361241680581No Hit
CGGCTCCGGGACGGC680050.6361344577118616No Hit
ACGGACAGGATTGACAGA660340.6176972690323517No Hit
TGAGGTAGTAGGTTGTGTGGT573670.5366241516882048No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGC538830.5040340119827695No Hit
TCCTGTACTGAGCTGCCCCGAG533740.4992727085642659No Hit
TGAGGTAGTAGATTGTATAGT529110.4949416997572577No Hit
AGGCTGAGGCAGGAGAA519420.4858774502238No Hit
TGAGGTAGTAGGTTGTGTGGTT518710.48521329984518746No Hit
TGAGGTAGTAGATTGTATAGTT518700.4852039456145028No Hit
GCGCACGTGTTAGGA505770.4731089253392078No Hit
TCAAGGCCGAGAGCTGATGACGAGTC493350.4614909708288316No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT481560.4504623328515905No Hit
TCCTGTACTGAGCTGCCCCGA447160.41828377929628124No Hit
TGAGGGGCAGAGAGCGAGACTTT419110.39204516222574565No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC406940.3806610634824866No Hit
AAGCTGCCAGTTGAAGAACTGT404070.37797639927598253No Hit
CCCGGACACGGACAGGATTGACAGA396520.37091395510904696No Hit
AGGCTGAGGCAGGAGA377650.35326252180705026No Hit
TATTGCACTTGTCCCGGCCTGT357540.33445116390015295No Hit
TGAGGTAGTAGTTTGTGCTGTT352070.3293343997156314No Hit
CGCGACCTCAGATCAGA330270.3089421768230226No Hit
CGGACAGGATTGACAG306340.2865575027945764No Hit
CGCACGTGTTAGGA301730.2822452024489376No Hit
TGAGGGGCAGAGAGCGAGACT298610.2793266824753165No Hit
TAGCTTATCAGACTGATGTTGAC295710.27661395557675844No Hit
CAAGGCCGAGAGCTGATGACGAGTC286920.2683915868049222No Hit
CAAGGCCGAGAGCTGATGACGAGTCG279730.2616658949426352No Hit
AGCGCACGTGTTAGGA268470.25113303119168223No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT260100.2433035401086026No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGT244550.22875771139392068No Hit
TGAGGTAGTAGTTTGTACAGT244290.22851450139611892No Hit
CGGGTGTTGACGCGATGTGA225150.21061050386563582No Hit
TGGAGTGTGACAATGGTGTTC221910.20757973312379857No Hit
CGGCTCCGGGACGGT216430.20245361470859233No Hit
AAGGCCGAGAGCTGATGACGAGTCGT214180.20034891280453865No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAT212870.1991235085848452No Hit
TCAGTGCACTACAGAACTTTGC212350.1986370885892417No Hit
ACGATGTCGACTAGCCGTTGGGAT211310.1976642485980347No Hit
GCGCACGTGTTAGGAC208310.1948579793926298No Hit
TCCTGTACTGAGCTGCCCCGAGA193480.18098565528724503No Hit
AAAAGCTGGGTTGAGAGGGCGA187290.1751953864934263No Hit
TCCTGTACTGAGCTGCCCCGAGT185950.1739419195816788No Hit
AAGGCCGAGAGCTGATGACGAGTC184170.17227686651980523No Hit
GGTGGGCGTAGCTC179880.1682639015560763No Hit
TCAAGGCCGAGAGCTGATGACGAGTCG174830.1635400150603114No Hit
TGGCTCAGTTCAGCAGGAAC171610.1605279527798435No Hit
CCCATAAAGTAGAAAGCACT171360.16029409701272643No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT170670.1596486550954833No Hit
TCAAGGCCGAGAGCTGATGACGAGTCGC164220.15361517630386284No Hit
GCGGACGTGGTGGAATTGGTAGACACAC163950.15336261207537638No Hit
TCGTACCGTGAGTAATAATGCG161980.15151982863049385No Hit
GCGGGTGTTGACGCGATGTGA158890.14862937134892684No Hit
TGAGGTAGTAGGTTGTATAG156470.14636564752323358No Hit
CGGACAGGATTGAC155380.14534603637860313No Hit
CGGACAGGATTGACAGAT152440.14259589255730634No Hit
CGGGTGTTGACGCGATGTGAT152410.14256782986525232No Hit
TGAGGTAGTAGGTTGTATAGC151540.1417540117956849No Hit
GCGGGACATGTGGCGTACGGAAGAC150750.14101502757159495No Hit
AAACCGTTACCATTACTGAG148350.13877001220727103No Hit
AGCGCACGTGTTAGGAC148020.1384613225946765No Hit
AAGGCCGAGAGCTGATGACGAGTCG147600.13806844490591982No Hit
GCGGGGTGGAGCAGTCTGGTAGCTCGTCGGG145280.1358982633870734No Hit
TAGCTTATCAGACTGATGTTGA145180.13580472108022656No Hit
CGGTCGGGCTGGGGCG144470.13514057070161406No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC142660.13344745494768645No Hit
CGGAGTGATTCATGACTGGGGTGAAGTC140220.13116502266062383No Hit
TTCAAGTAATCCAGGATAGGCC139210.13022024536147087No Hit
TGGCGGAGCGCTGAGAAGACGGTC138220.12929417652368724No Hit
AGGCTGAGGCAGGAG134280.12560860963392217No Hit
GGACAGGATTGACAGA132220.1236816381128775No Hit
TGAGGTAGTAGGTTGTGTGG130080.12167983274635535No Hit
CACGGACAGGATTGACAG129080.12074440967788708No Hit
CAAGGCCGAGAGCTGATGACGAGTCGT128830.12051055391077001No Hit
GTCTGAGCGTCGCT128360.12007090506858989No Hit
CCGGACACGGACAGGATTGACAG127330.11910741930806756No Hit
TGGAGTGTGACAATGGTGTTTG125750.11762945085988766No Hit
GCGGGATGGAGCAGTCTGGTAGCTCGTCGGG125220.11713367663359947No Hit
CGGGTGTTGACGCGATGTG125180.11709625971086074No Hit
CATTGCACTTGTCTCGGTCTGA124870.11680627855963556No Hit
TGAGGTAGTAGGTTGTATAGTC123970.1159643977980141No Hit
CGGAGTGATTCATGACTGGGGTGA123670.11568377087747361No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC123170.11521605934323945No Hit
TCCTGTACTGAGCTGCCCCG122500.11458932588736571No Hit
TGAGGTAGTAGTTTGTACAGTT121720.11385969589396043No Hit
TTCACAGTGGCTAAGTTCCG119730.11199820398770854No Hit
GCGCGGGACATGTGGCGTACGGAAG114600.1071994836464662No Hit
TGAGGTAGTAGGTTGTGTGGC110250.10313039329862914No Hit
CACGGACAGGATTGACAGAT109450.1023820548438545No Hit
TGGAGAGAAAGGCAGTTCCTGA108820.10179273831071949No Hit
CGCGACCTCAGATCAGACGT107280.10035218678527832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)