FastQCFastQC Report
Wed 8 May 2024
EGAF00002167656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12611388
Total Bases286.7 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT134646710.67659642221776No Hit
TGAGGTAGTAGGTTGTATAGT2810812.2287871882143344No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2696572.1382023929483416No Hit
TCAGTGCACTACAGAACTTTGT2321751.8409948215057692No Hit
TCCTGTACTGAGCTGCCCCGAG2299181.8230982981413308No Hit
TGAGGTAGTAGGTTGTATAGTT2101541.666382796247328No Hit
TCCTGTACTGAGCTGCCCCGA2066651.6387173243738122No Hit
TATTGCACTTGTCCCGGCCTGT2022341.603582412974686No Hit
AAACCGTTACCATTACTGAGC2008441.5925606285366842No Hit
TTCAAGTAATCCAGGATAGGCT1687741.3382666523304176No Hit
TGAGGTAGTAGATTGTATAGT1633301.2950993181717985No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1486141.178411131272783No Hit
TGAGGTAGTAGATTGTATAGTT1387971.1005687875117314No Hit
TGAGGTAGTAGGTTGTGTGGT1329881.0545072437704714No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1291911.024399534769686No Hit
TGAGGTAGTAGGTTGTGTGGTT1054260.835958738245148No Hit
CGCGACCTCAGATCAGA1022930.8111161118823716No Hit
CATTGCACTTGTCTCGGTCTGA918570.7283655058428145No Hit
TGAGGGGCAGAGAGCGAGACTTT906300.7186362040403482No Hit
AAGCTGCCAGTTGAAGAACTGT821700.6515539764536624No Hit
TGAGGTAGTAGTTTGTGCTGTT819820.6500632602850693No Hit
TGAGGGGCAGAGAGCGAGACT773530.6133583393041274No Hit
TGAGGTAGTAGTTTGTACAGT731810.5802771273074779No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT724270.5742984039504613No Hit
TGGAGTGTGACAATGGTGTTT710190.5631338913686582No Hit
TCCTGTACTGAGCTGCCCCGAGA690410.5474496542331423No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT648980.5145983931348398No Hit
TGAGGTAGTAGGTTGTATAG585630.46436601585804826No Hit
TCCTGTACTGAGCTGCCCCG559900.44396382063576184No Hit
AAACCGTTACCATTACTGAG554000.439285509255603No Hit
TGAGGTAGTAGTTTGTACAGTT521940.41386404097629853No Hit
TAGCTTATCAGACTGATGTTGAC519030.4115566026515083No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC505140.400542747554829No Hit
AAAAGCTGGGTTGAGAGGGCGA501840.39792606491846894No Hit
TCCTGTACTGAGCTGCCCCGAGT491980.3901077343746779No Hit
TCGTACCGTGAGTAATAATGCG471670.3740032421490799No Hit
CCCATAAAGTAGAAAGCACT468340.37136277148875285No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC467860.3709821631052823No Hit
TGAGGTAGTAGGTTGTATAGC422630.33511775230450447No Hit
CGCGACCTCAGATCAGACGT418610.33193015709293855No Hit
TATTGCACTTGTCCCGGCCTGC387120.30696066126900545No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG385790.3059060588731391No Hit
TAGCTTATCAGACTGATGTTGA380420.30164800258306224No Hit
TCAGTGCACTACAGAACTTTGC364850.289302018144236No Hit
TGTAAACATCCCCGACTGGAAGCT340230.26977997980872526No Hit
TGGCTCAGTTCAGCAGGAAC337050.26725844926823283No Hit
TTCAAGTAATTCAGGATAGGT331340.26273079537319766No Hit
TGAGGTAGTAGGTTGTATAGTC330710.2622312468698925No Hit
TTCAAGTAATCCAGGATAGGCC318080.25221648877982344No Hit
TGAGGTAGTAGGTTGTGTGG314250.2491795510533813No Hit
AGAGGTAGTAGGTTGCATAGT299450.2374441258963724No Hit
TGGCTCAGTTCAGCAGGAACAG298280.23651639296166288No Hit
CTAGACTGAAGCTCCTTGAGG298050.23633401811124993No Hit
TTCACAGTGGCTAAGTTCCG293360.23261515703108968No Hit
TGAGAACTGAATTCCATGGGTT284780.22581178217655345No Hit
AAACCGTTACCATTACTGAGTT270990.2148772204930972No Hit
CATTGCACTTGTCTCGGTCTG266690.21146760372450674No Hit
TCCTGTACTGAGCTGCCCCGAA257210.20395058815096323No Hit
AGAGGTAGTAGGTTGCATAGTT253680.20115153066419017No Hit
TGAGGTAGTAGATTGTATAG250810.1988758097046891No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT249370.19773398455427746No Hit
CAACGGAATCCCAAAAGCAGCT246590.1955296276666771No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT246490.1954503342534541No Hit
TGAGGTAGTAGTTTGTGCTGT245550.19470497616915758No Hit
TGAGGTAGTAGATTGTATAGC239500.18990772466916409No Hit
TGAGGGGCAGAGAGCGAGACTT235660.18686285760139962No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT226390.17951235819562447No Hit
TGAGGTAGTAGGTTGTGTGGC225310.1786559893328157No Hit
TGTAAACATCCCCGACTGGAAG223510.1772287078948011No Hit
TGGAGAGAAAGGCAGTTCCTGA219740.17423934621629275No Hit
TGAGATGAAGCACTGTAGCT218090.17293100489811272No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC213790.1695213881295223No Hit
AAACCGTTACCATTACTGAGG211760.16791173184109473No Hit
TGAGGTAGTAGATTGTATAGTC211250.16750733543365726No Hit
AAACCGTTACCATTACTGAGA199420.15812692464937245No Hit
TGTAAACATCCTTGACTGGAAGCT197870.15689787674441544No Hit
AGCTCGGTCTGAGGCCCCTCAGT194000.15382922165268406No Hit
AAACCGTTACCATTACTG188190.14922227434442584No Hit
TGAGGTAGTAGGTTGTAT187080.1483421174576502No Hit
TAGCTTATCAGACTGATGTTG182670.14484527793451443No Hit
TGAGGTAGTAGGTTGTGTGGTC182030.144337800089887No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC178260.14134843841137865No Hit
TATTGCACTTGTCCCGGCCTG168470.13358561325684373No Hit
CGTGTATTTGACAAGCTGAGTTG166960.13238828271717595No Hit
TGTAAACATCCCCGACTGGAAGC166450.13198388630973845No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC158030.1253073809163591No Hit
TGAGATGAAGCACTGTAGCTC156430.12403868630479055No Hit
CAACGGAATCCCAAAAGCAGCTG151800.12036740127256414No Hit
TGGCTCAGTTCAGCAGGAACA147920.11729081683951045No Hit
TAGCAGCACGTAAATATTGGCG147780.1171798060609982No Hit
GGCTGGTCCGATGGTAGTGGGT145320.11522918809571157No Hit
AAAAGCTGGGTTGAGAGGGCGAT144880.11488029707753024No Hit
AAGCTGCCAGTTGAAGAACT142090.11266801084860761No Hit
GGCTGGTCCGATGGTAGTGGGTT137740.10921874737340569No Hit
AAAAGCTGGGTTGAGAGGGCG136700.10839409587588614No Hit
AAAAGCTGGGTTGAGAGGGCGT136460.10820379168415087No Hit
AAGCTGCCAGTTGAAGAACTGC133360.10574569587423685No Hit
TGAGGTAGTAGATTGTAT133110.10554746234117925No Hit
GCATTGGTGGTTCAGTGGTAGAATT132120.10476245755027123No Hit
TGTCAGTTTGTCAAATACCCCAA132040.1046990228196928No Hit
CGCGACCTCAGATC131910.10459594138250286No Hit
TGAGGGGCAGAGAGCGAGACC131260.10408053419655315No Hit
GGCTGGTCCGATGGTAGTGGGTTATC131110.1039615940767186No Hit
TGAGGTAGTAGTTTGTGCTGTC130640.10358891503457035No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG130610.10356512701060344No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA129720.10285941563291845No Hit
TTCAAGTAATCCAGGATAGGCA128100.1015748623387053No Hit
TCCTGTACTGAGCTGCCCCA128090.10156693299738301No Hit
TGAGGTAGTAGTTTGTGCT127920.10143213419490384No Hit
TCAGTGCATCACAGAACTTTGT127620.10119425395523475No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC126730.10048854257754976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)