FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15751014
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT13780498.748954194314093No Hit
TCCTGTACTGAGCTGCCCCGAG3642432.312505086974083No Hit
TGAGGTAGTAGGTTGTATAGT3634282.3073308169239137No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3412922.1667938330827465No Hit
TGAGGTAGTAGGTTGTATAGTT2803691.7800060364367654No Hit
TCCTGTACTGAGCTGCCCCGA2770061.7586550300825077No Hit
TCAGTGCACTACAGAACTTTGT2543831.615026181806454No Hit
TGAGGTAGTAGATTGTATAGT2246361.426168499374072No Hit
AAACCGTTACCATTACTGAGC2231421.4166833957483627No Hit
TGAGGTAGTAGATTGTATAGTT2092481.3284732017887864No Hit
CGCGACCTCAGATCAGA1932151.2266829297466182No Hit
TTCAAGTAATCCAGGATAGGCT1622761.030257480566013No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1558730.989606129484743No Hit
TATTGCACTTGTCCCGGCCTGT1550480.9843683714584979No Hit
TGAGGTAGTAGGTTGTGTGGT1448470.9196042870636773No Hit
TGAGGTAGTAGGTTGTGTGGTT1241380.7881270374085123No Hit
TGAGGTAGTAGGTTGTATAG1226750.7788387465086375No Hit
AAACCGTTACCATTACTGAGG1140150.7238581592270822No Hit
TGAGGTAGTAGTTTGTGCTGTT1007620.6397175445339582No Hit
TCCTGTACTGAGCTGCCCCG1000940.6354765477321015No Hit
TGAGGTAGTAGTTTGTACAGT987230.6267723462121232No Hit
TCCTGTACTGAGCTGCCCCGAGA851080.540333466785059No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT813530.516493731768634No Hit
AAGCTGCCAGTTGAAGAACTGT801600.5089196162228032No Hit
AAAAGCTGGGTTGAGAGGGCGA797110.5060690060970043No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG735160.46673820491810875No Hit
CATTGCACTTGTCTCGGTCTGA726830.461449656511003No Hit
TGAGGGGCAGAGAGCGAGACTTT719480.45678329026943915No Hit
TGAGGTAGTAGTTTGTACAGTT719460.45677059267422404No Hit
TAGCTTATCAGACTGATGTTGAC673080.42732486937031483No Hit
AAACCGTTACCATTACTGAG663450.42121097727422496No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC623180.39564436930854097No Hit
TGGAGTGTGACAATGGTGTTT602430.3824706142728335No Hit
TGGAGAGAAAGGCAGTTCCTGA602430.3824706142728335No Hit
TGAGGGGCAGAGAGCGAGACT595890.3783185006374828No Hit
TGAGGTAGTAGGTTGTATAGC594380.3773598321987397No Hit
CGCGACCTCAGATCAGACGT585640.3718109830897236No Hit
TCCTGTACTGAGCTGCCCCGAGT572060.3631893159386437No Hit
TGAGGTAGTAGATTGTATAG557880.3541867209311096No Hit
TCGTACCGTGAGTAATAATGCG536780.34079075797913705No Hit
TGAGGTAGTAGGTTGTATAGTC478220.3036121991892078No Hit
CCCATAAAGTAGAAAGCACT471450.29931406320888293No Hit
AGAGGTAGTAGGTTGCATAGT444280.2820643801091155No Hit
TAGCTTATCAGACTGATGTTGA434680.27596953440584837No Hit
TCAGTGCACTACAGAACTTTGC429710.27281418199488616No Hit
TGAGGTAGTAGGTTGTGTGG427870.2716460032350933No Hit
TGTAAACATCCCCGACTGGAAGCT427240.2712460289858164No Hit
CTAGACTGAAGCTCCTTGAGG394050.2501743697262919No Hit
TGGCTCAGTTCAGCAGGAAC388490.24664443825648305No Hit
TTCAAGTAATTCAGGATAGGT372620.23656889645326962No Hit
TCCTGTACTGAGCTGCCCCGAA359840.2284551331107953No Hit
TGAGGTAGTAGATTGTATAGC358580.22765518461224146No Hit
TTCACAGTGGCTAAGTTCCG357400.22690602649454822No Hit
AGAGGTAGTAGGTTGCATAGTT356910.2265949354117773No Hit
TGAGGTAGTAGTTTGTGCTGT352000.22347767578646047No Hit
TGAGGTAGTAGATTGTATAGTC347940.2209000639577871No Hit
TGAGAACTGAATTCCATGGGTT328600.2086214893847469No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT323220.20520583627187428No Hit
TTCAAGTAATCCAGGATAGGCC322530.20476776923695197No Hit
TATTGCACTTGTCCCGGCCTGC319570.20288852514511127No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC315080.2000379150193124No Hit
TGGCTCAGTTCAGCAGGAACAG306880.1948319009811051No Hit
TGAGGTAGTAGGTTGTAT305720.19409544045862698No Hit
AAACCGTTACCATTACTG303760.19285107612754326No Hit
TGTAAACATCCCCGACTGGAAG291950.18535314615300325No Hit
AAATGGATTTTTGGAAATAGGAGA287150.18230572330136968No Hit
CATTGCACTTGTCTCGGTCTG285310.18113754454157682No Hit
CAACGGAATCCCAAAAGCAGCT278780.17699177970383367No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC275630.17499190845744914No Hit
AAAAGCTGGGTTGAGAGGGCG275410.17485223491008262No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC274880.1745157486368814No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT273040.17334756987708855No Hit
AAACCGTTACCATTACTGAGA271660.17247143580724392No Hit
TGAGGTAGTAGGTTGTATAGG270510.17174132408237336No Hit
TGAGGTAGTAGGTTGTGTGGC265350.1684653445168673No Hit
GGCTGGTCCGATGGTAGTGGGT264180.1677225351967816No Hit
GGCTGGTCCGATGGTAGT257660.16358311915664603No Hit
GGCTGGTCCGATGGTAGTG255310.1620911517188671No Hit
TGAGATGAAGCACTGTAGCT249130.1581675947973889No Hit
AAAAGCTGGGTTGAGAGGGCGAT234540.14890469908794443No Hit
AAATGGATTTTTGGAGCAGGGAGA233880.14848567844584482No Hit
TAGCTTATCAGACTGATGTTG229680.14581918345066547No Hit
TGAGGTAGTAGGTTGTGTGGTC229640.14579378826023517No Hit
TTCAAGTAATCCAGGATAGGCG227640.14452402873872122No Hit
TCCTGTACTGAGCTGCCCCGG225210.14298127092008173No Hit
AAATGGATTTTTGGAAATAGGAGAT223900.14214957843349008No Hit
TCAGTGCACTACAGAACTTTGG223430.1418511849459343No Hit
AGAAGCCGGCGGGAGC221320.14051158865073704No Hit
CGTGTATTTGACAAGCTGAGTTG214200.13599124475414726No Hit
TGTAAACATCCTTGACTGGAAGCT212470.13489290276803767No Hit
TGAGGGGCAGAGAGCGAGACTT212100.13465799725655758No Hit
AAAAGCTGGGTTGAGAGGGCGAA209540.13303270506901968No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA209210.13282319474796986No Hit
TATTGCACTTGTCCCGGCCTG204220.1296551447417925No Hit
AGCTCGGTCTGAGGCCCCTCAGT203120.1289567770049598No Hit
AAACCGTTACCATTACTGAGTT201910.12818857249444385No Hit
AGAGGTAGTAGGTTGCATAG201750.12808699173272273No Hit
TGAGGTAGTAGATTGTAT201520.12794096938774863No Hit
GGCTGGTCCGATGGTAGTGGGTT199700.1267854882231709No Hit
TGTAAACATCCCCGACTGGAAGC197360.12529986958299955No Hit
TCCTGTACTGAGCTGCCCCA192700.12234132989787196No Hit
TGGAGAGAAAGGCAGTTCCTG192240.12204928520792376No Hit
TGAGGTAGTAGTTTGTGCTG187840.119255814260593No Hit
AAGCTGCCAGTTGAAGAACT183510.11650678489651524No Hit
AAAAGCTGGGTTGAGAGGGCGT180720.11473547036400322No Hit
TACAGTACTGTGATAACTGAAG180120.11435454250754903No Hit
CAACGGAATCCCAAAAGCAGCTG177980.1129958998195291No Hit
AAATGGATTTTTGGAGCAGGGAGAT175760.11158646675064857No Hit
CGCGACCTCAGATC175470.11140235162002904No Hit
TGAGGTAGTAGTTTGTGCTGTC174970.11108491173965054No Hit
GGCTGGTCCGATGGTAGTGGGTTATC173410.11009449931286965No Hit
AAATGGATTTTTGGAGCAGGGA170680.10836127756600304No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT166570.10575192174929182No Hit
TGAGATGAAGCACTGTAGCTC166320.10559320180910257No Hit
GGCTGGTCCGATGGTAGTGG165950.1053582962976225No Hit
TGAGGTAGTAGTTTGTACAGC162860.1033965178368834No Hit
TGAGGTAGTAGTTTGTGCT161430.10248863977900088No Hit
TGAGGTAGTAGATTGTATAGG160240.10173313286370009No Hit
TATTGCACTTGTCCCGGCCTGG159490.10125697304313233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)