FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13391044
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8855216.612785381035264No Hit
CGCGACCTCAGATCAGA3400812.539615283169856No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2095701.565001205283173No Hit
TCAGTGCACTACAGAACTTTGT1833341.3690792144361559No Hit
TTCAAGTAATCCAGGATAGGCT1729851.2917962184277791No Hit
TCCTGTACTGAGCTGCCCCGAG1674491.2504551549528178No Hit
TGAGGTAGTAGGTTGTATAGT1613471.20488738592749No Hit
AAACCGTTACCATTACTGAGC1582331.1816330377228244No Hit
TGAGGTAGTAGGTTGTATAGTT1417511.058550774681944No Hit
TATTGCACTTGTCCCGGCCTGT1227470.916635028605686No Hit
TCCTGTACTGAGCTGCCCCGA1218420.9098767803316903No Hit
TGAGGTAGTAGATTGTATAGTT1070940.7997434703373388No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1066050.7960917759660859No Hit
TAGCTTATCAGACTGATGTTGAC995690.7435491960148888No Hit
TGAGGTAGTAGATTGTATAGT990690.7398153571894768No Hit
AAACCGTTACCATTACTGAG755780.5643921414939717No Hit
AAGCTGCCAGTTGAAGAACTGT719340.5371799241343692No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT700710.5232676406708843No Hit
TGAGGTAGTAGTTTGTGCTGTT675590.5045088344120144No Hit
TGAGGTAGTAGTTTGTACAGT619520.4626375658238447No Hit
TGAGGGGCAGAGAGCGAGACTTT612040.4570517429410284No Hit
TGAGGTAGTAGGTTGTGTGGT580300.4333493340773132No Hit
AAACCGTTACCATTACTGAGG574250.42883138909856466No Hit
CGCGACCTCAGATCAGACGT557590.416390238132292No Hit
TGAGGTAGTAGGTTGTATAG527130.3936436920078823No Hit
CCCATAAAGTAGAAAGCACT522250.3899994653142802No Hit
CGCGACCTCAGATC495910.37032960238200996No Hit
TGAGGTAGTAGGTTGTGTGGTT495140.3697545912028965No Hit
TCCTGTACTGAGCTGCCCCG461490.344625855907874No Hit
TAGCTTATCAGACTGATGTTGA460630.3439836356299031No Hit
GGCTGGTCCGATGGTAGT446380.33334219497747897No Hit
TTCACAGTGGCTAAGTTCCG441890.32998920771225904No Hit
CATTGCACTTGTCTCGGTCTGA415690.3104238922671003No Hit
TGTAAACATCCCCGACTGGAAGCT410650.30666018273108503No Hit
GGCTGGTCCGATGGTAGTG410510.3065556352439735No Hit
GGCTGGTCCGATGGTAGTGGGT403780.301529888184969No Hit
TCCTGTACTGAGCTGCCCCGAGA394910.29490605810868814No Hit
TGGCTCAGTTCAGCAGGAAC388870.2903955808075905No Hit
TTCAAGTAATCCAGGATAGGCC378570.28270387282724185No Hit
CGCGACCTCAGATCAGAC365200.27271958780809025No Hit
TGAGGGGCAGAGAGCGAGACT354840.2649830737618366No Hit
TCAGTGCACTACAGAACTTTGC351230.2622872421298892No Hit
AAAAGCTGGGTTGAGAGGGCGA350420.2616823602401725No Hit
TGAGGTAGTAGTTTGTACAGTT347850.2597631670839107No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC323860.24184820839958407No Hit
TCGTACCGTGAGTAATAATGCG315130.2353289258104148No Hit
TGAGGTAGTAGGTTGTATAGC292760.21862373090552165No Hit
TCCTGTACTGAGCTGCCCCGAGT291980.21804125204875738No Hit
TATTGCACTTGTCCCGGCCTGC277520.20724299016566597No Hit
GGCTGGTCCGATGGTAGTGGGTT275360.20562997179308798No Hit
TGAGATGAAGCACTGTAGCT274850.20524912023289596No Hit
TGTAAACATCCCCGACTGGAAG268700.20065649847763925No Hit
TGTAAACATCCTTGACTGGAAGCT260210.1943164401520897No Hit
TGAGGTAGTAGGTTGTATAGTC259010.19342031883399083No Hit
TGAGGTAGTAGATTGTATAG254760.19024655583239067No Hit
TTCAAGTAATTCAGGATAGGT250010.1866994089482493No Hit
TAGCTTATCAGACTGATGTTG237860.1776261806024982No Hit
AAACCGTTACCATTACTG234250.17493034897055076No Hit
TGAGAACTGAATTCCATGGGTT233770.17457190044331122No Hit
CAACGGAATCCCAAAAGCAGCT231340.172757254774161No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG229510.17139066976406023No Hit
AAACCGTTACCATTACTGAGTT225830.16864256438855701No Hit
GGCTGGTCCGATGGTAGTGG223790.16711915814778894No Hit
AGCTCGGTCTGAGGCCCCTCAGT223390.16682045104175597No Hit
TGAGGTAGTAGTTTGTGCTGT214080.15986804314883887No Hit
AGAGGTAGTAGGTTGCATAGT213820.15967388352991746No Hit
TGGAGAGAAAGGCAGTTCCTGA213070.15911380770610567No Hit
CATTGCACTTGTCTCGGTCTG211850.15820275103270515No Hit
GCATTGGTGGTTCAGTGGTAGAATT203960.15231075336620506No Hit
TGTAAACATCCCCGACTGGAAGC203350.1518552250295048No Hit
TGAGATGAAGCACTGTAGCTC202040.15087695925724687No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC199120.14869639738320628No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT198360.14812885388174366No Hit
CGCGACCTCAGATCAGG198250.14804670942758458No Hit
AGAGGTAGTAGGTTGCATAGTT197180.14724766791894645No Hit
TGAGGTAGTAGGTTGTGTGG191770.14320765430985066No Hit
TGAGGTAGTAGATTGTATAGTC191660.1431255098556916No Hit
GTTTCCGTAGTGTAGTGGTTATC191580.143065768434485No Hit
TGAAAAGAACTTTGAAGAGAG186130.13899588411478597No Hit
TACAGTACTGTGATAACTGAAG186000.13889880430532525No Hit
GGCTGGTCCGATGGTAGTGGG181150.13527698064467567No Hit
TGAGGTAGTAGATTGTATAGC180060.13446300378073583No Hit
TAGCTTATCAGACTGATGTTGAT179780.13425390880651278No Hit
TGAGGGGCAGAGAGCGAGACTT175960.131401255943898No Hit
TGAGGTAGTAGGTTGTAT175100.13075903566592717No Hit
CAACGGAATCCCAAAAGCAGCTG174540.13034084571748103No Hit
TTCAAGTAATCCAGGATAGGCG172220.12860834450248987No Hit
CTAGACTGAAGCTCCTTGAGG168390.12574822396222432No Hit
TGGCTCAGTTCAGCAGGAACAG165320.12345564692342134No Hit
AGAAGCCGGCGGGAGC157650.1177279381652394No Hit
GGCTGGTCCGATGGTAGTGGGTTATC157060.11728734518384079No Hit
ATTTTTGGAGCAGGGAGA153360.11452430445303592No Hit
TATTGCACTTGTCCCGGCCTG151000.11276193252744146No Hit
AAGCTGCCAGTTGAAGAACTGC139690.1043159891043596No Hit
AAACCGTTACCATTACTGAGA139100.103875396122961No Hit
AAGCTGCCAGTTGAAGAACT138270.1032555788779426No Hit
TCCTGTACTGAGCTGCCCCGAA138180.10318836977908519No Hit
TTTTTGGAGCAGGGAGA136720.1020980888420649No Hit
AGTTGGTCCGAGTGTTGTGGGT136620.10202341206555665No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT135560.10123183823456933No Hit
TGTCTGAGCGTCGCT134400.10036558762707373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)