FastQCFastQC Report
Wed 8 May 2024
EGAF00002167659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11422561
Total Bases258.9 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7912816.927351930972398No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2541642.225105210644093No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2330092.039901559729031No Hit
TATTGCACTTGTCCCGGCCTGT1863911.6317794231959015No Hit
TCCTGTACTGAGCTGCCCCGAG1765581.5456954005323325No Hit
TCCTGTACTGAGCTGCCCCGA1713991.5005303976927766No Hit
AAACCGTTACCATTACTGAGC1331711.1658593900264573No Hit
TTCAAGTAATCCAGGATAGGCT1241011.0864551303337318No Hit
TCAGTGCACTACAGAACTTTGT1158281.0140282901531452No Hit
TGGAGTGTGACAATGGTGTTT1146591.003794157895064No Hit
CGCGACCTCAGATCAGA846760.7413048614929699No Hit
TGAGGTAGTAGGTTGTATAGTT805750.7054022298502061No Hit
TAGCTTATCAGACTGATGTTGAC766520.6710579177471673No Hit
TGAGGTAGTAGGTTGTATAGT758460.6640017067976263No Hit
TGAGGTAGTAGTTTGTGCTGTT688090.6023955573535568No Hit
TGAGGTAGTAGATTGTATAGTT682310.5973353961515285No Hit
AAGCTGCCAGTTGAAGAACTGT676220.5920038422206719No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC669820.5864008955609867No Hit
TGAGGGGCAGAGAGCGAGACTTT614310.5378040878923738No Hit
CGCGACCTCAGATC559950.49021405970167287No Hit
TGAGGTAGTAGGTTGTGTGGT550030.4815294923791609No Hit
TGAGGTAGTAGGTTGTGTGGTT549570.481126780587996No Hit
TGAGGTAGTAGATTGTATAGT525570.46011573061417665No Hit
TCCTGTACTGAGCTGCCCCGAGA475500.416281427606296No Hit
TCCTGTACTGAGCTGCCCCG470680.4120617084032206No Hit
TGAGGGGCAGAGAGCGAGACT464140.4063361972853548No Hit
TAGCTTATCAGACTGATGTTGA447610.3918648366158867No Hit
TGAGGTAGTAGTTTGTACAGT439100.38441466847933664No Hit
TCCTGTACTGAGCTGCCCCGAGT436960.3825411831900044No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC425520.3725259160358172No Hit
CATTGCACTTGTCTCGGTCTGA421600.36909411120676No Hit
CGCGACCTCAGATCAGACGT404110.35378230853833914No Hit
TATTGCACTTGTCCCGGCCTGC402040.35197010547809726No Hit
AAACCGTTACCATTACTGAG378510.33137052189959854No Hit
TGTAAACATCCCCGACTGGAAGCT372920.32647669817652974No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC369630.32359643340928534No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT317810.27823007467414707No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG314310.27516596321963177No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT301280.263758714004679No Hit
CCCATAAAGTAGAAAGCACT292430.2560108893268331No Hit
TAGCTTATCAGACTGATGTTG280820.245846793901998No Hit
TCGTACCGTGAGTAATAATGCG270510.2368207970174114No Hit
AAAAGCTGGGTTGAGAGGGCGA262320.22965077621384558No Hit
TTCAAGTAATCCAGGATAGGCC261430.22887161644398307No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT256960.22495830838635927No Hit
TGAGAACTGAATTCCATGGGTT248990.21798088887422007No Hit
TGAGGTAGTAGTTTGTGCTGT229340.20077809170815547No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT221790.1941683655705581No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT218860.191603266552921No Hit
TGAGGTAGTAGTTTGTACAGTT215210.18840783603606934No Hit
TGAGGTAGTAGGTTGTATAG211470.1851336140818158No Hit
TCAGTGCACTACAGAACTTTGC209140.18309379131352418No Hit
TATTGCACTTGTCCCGGCCTG205080.1795394220262864No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT196940.17241317424349933No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC193550.16944536343469735No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT188370.16491047848201468No Hit
TGTAAACATCCTTGACTGGAAGCT182120.1594388508846659No Hit
TGGCTCAGTTCAGCAGGAAC177100.15504403959847535No Hit
CATTGCACTTGTCTCGGTCTG172620.1511219769366957No Hit
TGAGGTAGTAGGTTGTGTGG171320.14998387839644717No Hit
CGCGACCTCAGATCAGAC168990.14794405562815555No Hit
TGGAGTGTGACAATGGTGTTC163840.14343543448794013No Hit
TCCTGTACTGAGCTGCCCCGAA163340.14299770428015224No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT155640.13625665908021853No Hit
TGAGGGGCAGAGAGCGAGACTT152280.1333151120838838No Hit
AAACCGTTACCATTACTGAGTT152000.13306998316752258No Hit
AAACCGTTACCATTACTG151890.13297368252180924No Hit
AGCTCGGTCTGAGGCCCCTCAGT148040.1296031599218424No Hit
TTCAAGTAATTCAGGATAGGT147150.12882400015197992No Hit
CAACGGAATCCCAAAAGCAGCT146590.12833374231925748No Hit
TGTAAACATCCCCGACTGGAAG143210.12537468611461125No Hit
CTAGACTGAAGCTCCTTGAGG130780.11449271314900397No Hit
TGAGGTAGTAGGTTGTATAGTC130380.11414252898277366No Hit
TACAGTACTGTGATAACTGAAG129110.11303069425499238No Hit
TGGAGAGAAAGGCAGTTCCTGA129020.11295190281759054No Hit
TGAGGTAGTAGGTTGTATAGC128740.11270677390122932No Hit
AAGCTGCCAGTTGAAGAACT126950.11113969975734865No Hit
TAGCTTATCAGACTGATGTTGAT126630.11085955242436438No Hit
TGTAAACATCCCCGACTGGAAGC126290.11056189588306861No Hit
AAGCTGCCAGTTGAAGAACTGC123390.10802306067789877No Hit
GCATTGGTGGTTCAGTGGTAGAATT122950.10763785809504542No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA115990.1015446536026378No Hit
TGAGGTAGTAGTTTGTGCTGTC115420.10104564116575959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)