FastQCFastQC Report
Wed 8 May 2024
EGAF00002167660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12479853
Total Bases295.7 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6523325.227080799749804No Hit
AAACCGTTACCATTACTGAGT6423795.14732825779278No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3676302.945787903110718No Hit
TCCTGTACTGAGCTGCCCCGAG2802402.2455392703744184No Hit
TCCTGTACTGAGCTGCCCCGA2472211.9809608334328939No Hit
CGCGACCTCAGATCAGA1973951.581709335839132No Hit
TAGCTTATCAGACTGATGTTGAC1792541.436347046716015No Hit
TATTGCACTTGTCCCGGCCTGT1639371.3136132292583895No Hit
TTCAAGTAATCCAGGATAGGCT1429891.145758688023008No Hit
TGAGGTAGTAGGTTGTATAGT1366801.095205207945959No Hit
TGAGGTAGTAGGTTGTATAGTT1280781.0262781140130417No Hit
TCAGTGCACTACAGAACTTTGT1232570.9876478513008127No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1078790.8644252460345487No Hit
TGAGGTAGTAGATTGTATAGTT998900.800410068932703No Hit
TGGAGTGTGACAATGGTGTTT920680.7377330486184412No Hit
TGAGGTAGTAGTTTGTGCTGTT899720.7209379789970283No Hit
TGAGGTAGTAGATTGTATAGT866860.6946075406497175No Hit
TCCTGTACTGAGCTGCCCCGAGA851900.682620219965732No Hit
TGAGGTAGTAGGTTGTGTGGTT828660.6639982057480965No Hit
TAGCTTATCAGACTGATGTTGA828220.6636456374926852No Hit
TGAGGTAGTAGGTTGTGTGGT819750.656858698576017No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC734410.588476482856008No Hit
AAACCGTTACCATTACTGAGC692190.5546459561663106No Hit
TGAGGGGCAGAGAGCGAGACTTT690090.5529632440382111No Hit
TCGTACCGTGAGTAATAATGCG681670.5462163696960213No Hit
TCCTGTACTGAGCTGCCCCG675200.5410320137584954No Hit
AAGCTGCCAGTTGAAGAACTGT657520.526865180222876No Hit
TAGCTTATCAGACTGATGTTG583740.46774589412231055No Hit
CATTGCACTTGTCTCGGTCTGA578590.46361924295101875No Hit
TGAGGTAGTAGTTTGTACAGT549970.4406862805194901No Hit
AAACCGTTACCATTACTGAG510450.40901924085163505No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT482940.3869757119735305No Hit
TGAGGTAGTAGGTTGTATAG479870.38451574710054676No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT430350.34483579253697944No Hit
TCCTGTACTGAGCTGCCCCGAGT424130.33985175947184637No Hit
TGAGGGGCAGAGAGCGAGACT413990.331726663767594No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT405550.32496376359561285No Hit
CGCGACCTCAGATC397180.31825695382790165No Hit
AAAAGCTGGGTTGAGAGGGCGA391570.31376170857140706No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC388660.3114299503367548No Hit
TGTAAACATCCCCGACTGGAAGCT381690.3058449486544433No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG380150.30461095976050356No Hit
TGGCTCAGTTCAGCAGGAAC369870.29637368324771135No Hit
TATTGCACTTGTCCCGGCCTG350080.2805161246690967No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT340460.27280770053942144No Hit
TGAGGTAGTAGGTTGTGTGG330380.2647306823245434No Hit
CGCGACCTCAGATCAGAC313840.25147732108703524No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC289730.23215818327347285No Hit
CGCGACCTCAGATCAGACGT275810.2210042057386413No Hit
CATTGCACTTGTCTCGGTCTG273490.21914520948283606No Hit
TGTAAACATCCCCGACTGGAAGC267710.21451374467311435No Hit
TGAGGTAGTAGTTTGTACAGTT264390.21185345692773783No Hit
TACAGTACTGTGATAACTGAAG260880.20904092379934286No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT259540.20796719320331736No Hit
CCCATAAAGTAGAAAGCACT257350.2062123648411564No Hit
TGAGGTAGTAGATTGTATAG256360.20541908626648087No Hit
TGTAAACATCCCCGACTGGAAG254610.2040168261597312No Hit
CTAGACTGAAGCTCCTTGAGG250970.20110012513769193No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA249310.1997699812650037No Hit
TGAGAACTGAATTCCATGGGTT242510.1943211991359193No Hit
AAACCGTTACCATTACTG233360.18698938200634255No Hit
TTCACAGTGGCTAAGTTCCG221830.17775049113158625No Hit
TGAGGTAGTAGTTTGTGCTGT194980.1562358146365987No Hit
CAACGGAATCCCAAAAGCAGCTG190980.15303064867831376No Hit
TATTGCACTTGTCCCGGCCTGC190040.1522774346781168No Hit
TTCAAGTAATCCAGGATAGGCC189030.15146813027364986No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC182440.14618761935737545No Hit
TGGAGAGAAAGGCAGTTCCTGA180530.1446571526122944No Hit
TGTAAACATCCTTGACTGGAAGCT179710.14400009359084598No Hit
TTCAAGTAATTCAGGATAGGT176900.14174846450515083No Hit
TGGCTCAGTTCAGCAGGAACAG175450.14058659184527253No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC173060.1386715051851973No Hit
CAACGGAATCCCAAAAGCAGCT166740.1336073429711071No Hit
TATTGCACTTGTCCCGGCCTGTA163220.13078679692781636No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA157970.1265800166075674No Hit
AAAAGCTGGGTTGAGAGGGCG155620.12469698160707501No Hit
TGGAGAGAAAGGCAGTTCCTG151170.12113123447848305No Hit
TGAGGTAGTAGGTTGTATAGC148990.11938441903121776No Hit
TACCCTGTAGAACCGAATTTGT146060.11703663496677405No Hit
TGAGGTAGTAGGTTGTAT143760.11519366454076023No Hit
TACAGTATAGATGATGTACT143300.11482507045555744No Hit
TGAGGGGCAGAGAGCGAGACTT143060.11463276049806036No Hit
AGCTCGGTCTGAGGCCCCTCAGT141300.11322248747641499No Hit
TCAGTGCACTACAGAACTTTGC140160.11230901517830377No Hit
TGGAGTGTGACAATGGTGTTTG131430.10531374047434694No Hit
TGAGATGAAGCACTGTAGCTC130260.1043762294315486No Hit
AGAGGTAGTAGGTTGCATAGTT128910.10329448592062743No Hit
TGAGGTAGTAGATTGTAT125470.1005380431965024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)