FastQCFastQC Report
Wed 8 May 2024
EGAF00002167661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13773411
Total Bases308.1 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6981315.068686326139545No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2268741.647188194703549No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1752781.2725823690297198No Hit
TCCTGTACTGAGCTGCCCCGAG1672491.2142888932886704No Hit
TATTGCACTTGTCCCGGCCTGT1610801.1694996976420728No Hit
CGCGACCTCAGATCAGA1577131.1450540465248586No Hit
TCAGTGCACTACAGAACTTTGT1481161.075376317456874No Hit
AAACCGTTACCATTACTGAGC1459811.0598754368108234No Hit
TCCTGTACTGAGCTGCCCCGA1422731.0329540010096263No Hit
TTCAAGTAATCCAGGATAGGCT1395451.0131477235377642No Hit
TGAGGTAGTAGGTTGTATAGTT1202840.8733058209037688No Hit
TGAGGTAGTAGGTTGTATAGT1115330.809770361169067No Hit
TGAGGTAGTAGATTGTATAGTT974120.7072467379358679No Hit
TAGCTTATCAGACTGATGTTGAC951540.6908528323158294No Hit
TGGAGTGTGACAATGGTGTTT896920.6511967151782517No Hit
TGAGGTAGTAGTTTGTGCTGTT848770.6162380546111635No Hit
TGAGGGGCAGAGAGCGAGACTTT789440.5731623052561199No Hit
TGAGGTAGTAGATTGTATAGT745760.5414490281310853No Hit
AAGCTGCCAGTTGAAGAACTGT696790.5058950175813384No Hit
AAACCGTTACCATTACTGAGG585780.4252976985875176No Hit
TGAGGTAGTAGGTTGTGTGGTT582800.42313410962614856No Hit
TGAGGTAGTAGGTTGTGTGGT580340.4213480596781727No Hit
TCCTGTACTGAGCTGCCCCG512830.3723333312278273No Hit
TAGCTTATCAGACTGATGTTGA512370.3719993544082871No Hit
CGCGACCTCAGATC479650.34824343802707985No Hit
TGAGGTAGTAGTTTGTACAGT464730.3374109724889499No Hit
TGAGGGGCAGAGAGCGAGACT464560.33728754627303287No Hit
TATTGCACTTGTCCCGGCCTGC431200.3130669664907262No Hit
AAACCGTTACCATTACTGAG395680.28727814772970905No Hit
TGTAAACATCCCCGACTGGAAGCT393110.28541223375966923No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC392660.28508551730577125No Hit
TCCTGTACTGAGCTGCCCCGAGA380340.27614074683460765No Hit
CGCGACCTCAGATCAGACGT374450.27186439147136465No Hit
TCCTGTACTGAGCTGCCCCGAGT366610.2661722648078969No Hit
TTCAAGTAATCCAGGATAGGCC353210.25644337484737806No Hit
CATTGCACTTGTCTCGGTCTGA343810.24961863114373048No Hit
TAGCTTATCAGACTGATGTTG342290.24851505556611939No Hit
TCGTACCGTGAGTAATAATGCG330670.2400785106899083No Hit
TCAGTGCACTACAGAACTTTGC328480.23848849061427124No Hit
TGAGAACTGAATTCCATGGGTT316050.22946385612104364No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT291840.21188651090133012No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT280010.20329749834663322No Hit
CCCATAAAGTAGAAAGCACT276710.20090157768471442No Hit
AAAAGCTGGGTTGAGAGGGCGA275660.2001392392922857No Hit
TGAGGTAGTAGTTTGTACAGTT271790.19732947778876272No Hit
TGAGGTAGTAGGTTGTATAGTC261560.18990212373681437No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG259850.18866060121200187No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC251470.1825764148038565No Hit
TGGCTCAGTTCAGCAGGAAC243090.17649222839571113No Hit
TGAGGTAGTAGGTTGTATAG237000.17207066571962457No Hit
TGAGGTAGTAGGTTGTATAGC232120.16852760728624158No Hit
TGAGGTAGTAGTTTGTGCTGT229380.16653826710028474No Hit
TAGCTTATCAGACTGATGTTGAT216400.15711431249673738No Hit
CGCGACCTCAGATCAGAC208940.1516980797276724No Hit
TGAGGTAGTAGATTGTATAGTC207890.15093574133524368No Hit
TGAGGTAGTAGGTTGTGTGG204900.14876489200823237No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC203990.14810419873479416No Hit
TCCTGTACTGAGCTGCCCCGAA193600.14056067883257095No Hit
CTAGACTGAAGCTCCTTGAGG190470.13828818438656915No Hit
TATTGCACTTGTCCCGGCCTG190100.13801955085780857No Hit
TTCAAGTAATCCAGGATAGGCG185720.1348395107065345No Hit
TGAGGTAGTAGTTTGTGCTGTC185650.13478868814703926No Hit
TGTAAACATCCTTGACTGGAAGCT176650.12825435906907884No Hit
TGGAGTGTGACAATGGTGTTC174240.12650461094931387No Hit
TGTAAACATCCCCGACTGGAAG172760.1254300768342715No Hit
TGAGGGGCAGAGAGCGAGACTT167280.12145139646235781No Hit
CATTGCACTTGTCTCGGTCTG165960.12049302819759028No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG165750.12034056051910454No Hit
AGCTCGGTCTGAGGCCCCTCAGT165280.11999932333392216No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT162300.11783573437255304No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT161050.11692818866728075No Hit
TATTGCACTTGTCCCGGCCTGG160140.11626749539384253No Hit
AAGCTGCCAGTTGAAGAACTGC160000.11616585027485204No Hit
CAACGGAATCCCAAAAGCAGCT156660.1137408881503645No Hit
AAACCGTTACCATTACTG156080.11331978694311816No Hit
TTCAAGTAATCCAGGATAGGCA155370.1128043009825235No Hit
AAACCGTTACCATTACTGAGA152980.11106907359404289No Hit
TTCACAGTGGCTAAGTTCCG150890.10955165717482765No Hit
TGAGGTAGTAGATTGTATAGC149350.1084335608659322No Hit
TACAGTACTGTGATAACTGAAG147090.10679271823079992No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA142050.10313349394714207No Hit
TGAGGTAGTAGGTTGTGTGGC140660.1021243031228793No Hit
TGTAAACATCCCCGACTGGAAGC139270.10111511229861651No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT138770.1007520940165076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)