FastQCFastQC Report
Thu 9 May 2024
EGAF00002167665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9192789
Total Bases220.7 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT123101813.391126457922617No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2660772.894409955455303No Hit
TCAGTGCACTACAGAACTTTGT2411932.623719526250412No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2065242.2465869715926257No Hit
AAACCGTTACCATTACTGAGC2057452.2381129383041425No Hit
TCCTGTACTGAGCTGCCCCGAG1288241.4013592610468923No Hit
TGAGGTAGTAGGTTGTATAGT1227141.3348941219035921No Hit
TCCTGTACTGAGCTGCCCCGA1180791.284474167741694No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1180671.2843436306435403No Hit
TTCAAGTAATCCAGGATAGGCT1146121.2467598244667641No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1098161.194588497571303No Hit
TATTGCACTTGTCCCGGCCTGT1024451.1144060850303428No Hit
TGAGGTAGTAGGTTGTATAGTT995431.0828378634601534No Hit
TGAGGTAGTAGATTGTATAGT807210.8780904250059476No Hit
TGAGGTAGTAGATTGTATAGTT743340.8086120545135975No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC733770.7982017209358335No Hit
TGAGGGGCAGAGAGCGAGACT633630.6892685125265031No Hit
TGAGGTAGTAGGTTGTGTGGT629790.6850913253855822No Hit
TGAGGGGCAGAGAGCGAGACTTT611660.6653693454728483No Hit
TGAGGTAGTAGTTTGTGCTGTT567300.6171141315220006No Hit
TGAGGTAGTAGGTTGTGTGGTT551010.5993937204476248No Hit
AAGCTGCCAGTTGAAGAACTGT545730.5936500881288583No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT527020.5732971789083814No Hit
TGAGGTAGTAGTTTGTACAGT479050.5211149739214073No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC463820.50454764054739No Hit
TAGCTTATCAGACTGATGTTGAC456670.49676980511572716No Hit
TCCTGTACTGAGCTGCCCCGAGA447240.48651176481914243No Hit
AAACCGTTACCATTACTGAG443260.482182284397042No Hit
TCAGTGCACTACAGAACTTTGC415660.4521587518216724No Hit
CATTGCACTTGTCTCGGTCTGA395040.42972812712224767No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT389650.42386483579684037No Hit
CGCGACCTCAGATCAGA368910.40130367399926176No Hit
TCCTGTACTGAGCTGCCCCG344440.37468498406740325No Hit
TCCTGTACTGAGCTGCCCCGAGT330990.36005395098266696No Hit
CGCGACCTCAGATCAGACGT329670.35861804290297533No Hit
CCCATAAAGTAGAAAGCACT319780.34785961039680124No Hit
TCGTACCGTGAGTAATAATGCG304360.33108559328404036No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC302160.328692413151221No Hit
TAGCTTATCAGACTGATGTTGA298620.3248415687556845No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT278450.3029004581743365No Hit
TGTAAACATCCCCGACTGGAAGCT269890.2935888118393667No Hit
TGAGGTAGTAGTTTGTACAGTT261770.28475580153096086No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT253910.27620562160188816No Hit
AAAAGCTGGGTTGAGAGGGCGA234710.2553196858972832No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT232910.2533616294249765No Hit
TTCAAGTAATCCAGGATAGGCC231710.25205625844343865No Hit
TGAGGTAGTAGGTTGTATAG225080.24484408377044223No Hit
TTCACAGTGGCTAAGTTCCG219120.23836074122880446No Hit
TTCAAGTAATTCAGGATAGGT210430.22890767970416812No Hit
TGGCTCAGTTCAGCAGGAAC208340.22663415857798974No Hit
TGAGGTAGTAGGTTGTATAGC205690.22375146432709378No Hit
TATTGCACTTGTCCCGGCCTGC205030.22303351028724797No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT197150.2144615741751497No Hit
AAACCGTTACCATTACTGAGA191870.20871794185638332No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT190300.20701008148887134No Hit
AAACCGTTACCATTACTGAGTT186780.2031809932763604No Hit
AAACCGTTACCATTACTG183810.19995020009705433No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA179970.19577301295613334No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC179610.195381401661672No Hit
TGAGGTAGTAGGTTGTGTGG176070.19153055726613544No Hit
TGAGGTAGTAGGTTGTATAGTC173440.18866961919826508No Hit
TAGCTTATCAGACTGATGTTG167130.18180554345367875No Hit
CTAGACTGAAGCTCCTTGAGG165740.18029348873339746No Hit
TGGAGTGTGACAATGGTGTTT163470.17782416195998843No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG157280.17109062331355587No Hit
TGAGGGGCAGAGAGCGAGACTT156340.17006808271135126No Hit
TGAGGTAGTAGTTTGTGCTGT155190.16881710218737753No Hit
TGTAAACATCCTTGACTGGAAGCT154290.16783807395122416No Hit
TGAGAACTGAATTCCATGGGTT149170.16226849109666283No Hit
TCCTGTACTGAGCTGCCCCGAA148250.16126770667748383No Hit
TGGCTCAGTTCAGCAGGAACAG145360.1581239382302803No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA144130.156785932974204No Hit
AAACCGTTACCATTACTGAGG143120.15568724573140968No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC139470.15171674232923218No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT139180.15140127767536055No Hit
CGCGACCTCAGATC137470.14954112402666916No Hit
TGTAAACATCCCCGACTGGAAG134830.14666930786728596No Hit
TGAGGTAGTAGATTGTATAGC134720.146549648860645No Hit
CATTGCACTTGTCTCGGTCTG131750.14331885568133892No Hit
TGAGATGAAGCACTGTAGCT126390.13748819863047004No Hit
TGAGGTAGTAGATTGTATAGTC126100.13717273397659838No Hit
CAACGGAATCCCAAAAGCAGCT118420.1288183596947564No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG117940.12829621130214128No Hit
TGAGGGGCAGAGAGCGAGACC117360.127665281994398No Hit
TGAGGTAGTAGGTTGTGTGGC115770.1259356654438604No Hit
AGAGGTAGTAGGTTGCATAGT111560.12135598891696525No Hit
AAGCTGCCAGTTGAAGAACT109410.11901719924170999No Hit
TGAGGTAGTAGATTGTATAG109190.11877788122842807No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA106850.11623240781442933No Hit
AGCTCGGTCTGAGGCCCCTCAGT106610.11597133361812177No Hit
TGAGGTAGTAGGTTGTAT103930.11305600509268733No Hit
TGTAAACATCCCCGACTGGAAGC102430.11142429136576505No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA102390.11138077899971378No Hit
TGAGGTAGTAGGTTGTGTGGTC101880.11082599633256024No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT100630.10946623489345834No Hit
TGAGGTAGTAGTTTGTGCTGTC99550.10829140101007431No Hit
TATTGCACTTGTCCCGGCCTG98680.1073450070484594No Hit
AAGCTGCCAGTTGAAGAACTGC98330.10696427384551087No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA98230.10685549293038273No Hit
AGAGGTAGTAGGTTGCATAGTT98030.10663793110012641No Hit
AAACCGTTACTATTACTGAGT96570.1050497297392554No Hit
TTCAAGTAATCCAGGATAGGCA95500.1038857739473842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)