FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12298005
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10263778.345882116652254No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT5093844.14200514636317No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5076184.127645093655434No Hit
TCAGTGCACTACAGAACTTTGT2262961.8401033338334145No Hit
TTCAAGTAATCCAGGATAGGCT2212951.7994382015619605No Hit
AAACCGTTACCATTACTGAGC1968091.6003327368951306No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1669511.3575453904921977No Hit
TCCTGTACTGAGCTGCCCCGAG1537371.2500970685895803No Hit
TGAGGTAGTAGGTTGTATAGT1392651.1324194452677487No Hit
TATTGCACTTGTCCCGGCCTGT1304191.0604890793262811No Hit
TAGCTTATCAGACTGATGTTGAC1281711.0422096917345536No Hit
TGAGGTAGTAGATTGTATAGT1090200.8864852469973789No Hit
TGAGGTAGTAGGTTGTATAGTT1079320.8776382836077885No Hit
TCCTGTACTGAGCTGCCCCGA1077130.8758575069696264No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1028940.8366722895298871No Hit
TGAGGTAGTAGATTGTATAGTT1014600.8250118616800042No Hit
TGAGGTAGTAGTTTGTGCTGTT1014300.8247679196747765No Hit
CGCGACCTCAGATCAGA999340.8126033450140896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC841870.6845581864700819No Hit
TGAGGTAGTAGTTTGTACAGT833540.6777847301249268No Hit
AAGCTGCCAGTTGAAGAACTGT727890.5918764872839131No Hit
AAACCGTTACCATTACTGAG646550.5257356782665156No Hit
CCCATAAAGTAGAAAGCACT603900.49105525652331417No Hit
TGAGGGGCAGAGAGCGAGACTTT601310.48894922387818185No Hit
TCCTGTACTGAGCTGCCCCGAGA569900.4634084959308441No Hit
TAGCTTATCAGACTGATGTTGA563210.45796858921426686No Hit
TGTAAACATCCCCGACTGGAAGCT547970.44557633534870084No Hit
TTCAAGTAATCCAGGATAGGCC536530.43627401354935214No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT492140.4001787281758301No Hit
TCCTGTACTGAGCTGCCCCGAGT483110.39283607381847707No Hit
CGCGACCTCAGATCAGACGT479750.3901039233599271No Hit
TGAGGTAGTAGGTTGTGTGGT469890.38208636278811076No Hit
TCAGTGCACTACAGAACTTTGC466320.3791834529259014No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT450520.36633584065057706No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC425580.34605612861598284No Hit
TGAGGTAGTAGTTTGTACAGTT422010.34315321875377347No Hit
TGAGGTAGTAGGTTGTGTGGTT420730.3421123995314687No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT413410.3361602146039134No Hit
TGAGGGGCAGAGAGCGAGACT403750.3283052820355822No Hit
CATTGCACTTGTCTCGGTCTGA393710.32014135626062923No Hit
TTCAAGTAATTCAGGATAGGT387070.31474210654492335No Hit
AAACCGTTACCATTACTGAGTT383670.31197743048567633No Hit
TCCTGTACTGAGCTGCCCCG370560.30131716485722687No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT369540.30048776203945277No Hit
TGTAAACATCCTTGACTGGAAGCT364370.29628382814936244No Hit
CGCGACCTCAGATC339870.27636189772243547No Hit
TATTGCACTTGTCCCGGCCTGC329070.2675799855342391No Hit
TTCACAGTGGCTAAGTTCCG320530.2606357697854245No Hit
TCGTACCGTGAGTAATAATGCG315070.25619602529028085No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC286750.23316789999678808No Hit
CAACGGAATCCCAAAAGCAGCT285330.23201324117204375No Hit
TAGCTTATCAGACTGATGTTG273900.22271905077286924No Hit
TGAGGTAGTAGGTTGTATAGC272910.22191404215561794No Hit
TGGCTCAGTTCAGCAGGAAC269120.21883224148957492No Hit
GCATTGGTGGTTCAGTGGTAGAATT263050.21389648158380162No Hit
TGAGAACTGAATTCCATGGGTT256020.2081801072612997No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA253230.2059114466126823No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT249010.20247999573914632No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC239160.19447056656750422No Hit
GTTTCCGTAGTGTAGTGGTTATC231730.18842893623803209No Hit
TAGCTTATCAGACTGATGTTGAT222260.18072850027301177No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT221230.17989096605506338No Hit
TGAGGTAGTAGGTTGTATAG218790.17790690441254498No Hit
TACAGTATAGATGATGTACT214510.17442666513796345No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA213470.17358099951984082No Hit
TGAGGTAGTAGGTTGTATAGTC212000.1723856836942252No Hit
TGAGGTAGTAGATTGTATAGC208470.16951529943271285No Hit
TGAGGTAGTAGTTTGTGCTGTC205200.16685633157573118No Hit
AAAAGCTGGGTTGAGAGGGCGA204460.16625460796283625No Hit
TGTAAACATCCCCGACTGGAAG203270.1652869713420998No Hit
AAACCGTTACCATTACTG198470.16138389925845698No Hit
TGGAGTGTGACAATGGTGTTT193780.15757027257673092No Hit
TGAGGTAGTAGATTGTATAGTC193420.15727754217045772No Hit
TGAGGTAGTAGTTTGTGCTGT191090.15538292592985609No Hit
CATTGCACTTGTCTCGGTCTG186880.15195960645649437No Hit
TGAGATGAAGCACTGTAGCT186110.15133348864307666No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT185240.15062605682791638No Hit
CAACGGAATCCCAAAAGCAGCTG179570.14601555292911328No Hit
TGTAAACATCCCCGACTGGAAGC172880.1405756462125361No Hit
TACAGTACTGTGATAACTGAAG172590.14033983560748267No Hit
AGCTCGGTCTGAGGCCCCTCAGT172490.14025852160574012No Hit
TGAGGTAGTAGTTTGTACAGC166570.13544473270258062No Hit
AGAGGTAGTAGGTTGCATAGT165330.1344364390809729No Hit
TGAGGGGCAGAGAGCGAGACTT156740.12745156633128707No Hit
TGAGGTAGTAGATTGTAT154370.1255244244899884No Hit
AAGCTGCCAGTTGAAGAACTGC153070.12446734246733515No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC153030.12443481686663811No Hit
AGAGGTAGTAGGTTGCATAGTT145990.11871031114396195No Hit
TGAGGTAGTAGATTGTATAG143920.11702711130789101No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC143160.11640912489464755No Hit
AAGCTGCCAGTTGAAGAACT141400.11497799846397852No Hit
TGTAAACATCCCCGACTGGAAGCC140180.11398596764271929No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA139200.11318909042564221No Hit
CTAGACTGAAGCTCCTTGAGG139160.1131565648249452No Hit
AGCAGCATTGTACAGGGCTATG139070.11308338222337688No Hit
TGAGGTAGTAGGTTGTAT133540.10858671792701335No Hit
TCCTGTACTGAGCTGCCCCGAA132280.10756216150505711No Hit
TGAGATGAAGCACTGTAGCTC131460.10689538669076813No Hit
TTCAAGTAATCCAGGATAGGCA130660.10624487467682767No Hit
CGCGACCTCAGATCAGAC129020.1049113250482497No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT128920.10483001104650713No Hit
TATTGCACTTGTCCCGGCCTG128720.10466738304302202No Hit
TCAGTGCATCACAGAACTTTGT128260.10429333863500624No Hit
TTCAAGTAATTCAGGATAGGTT124220.10100825296460686No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT123160.10014632454613574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)