FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9425122
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7573498.035429143516657No Hit
TCAGTGCACTACAGAACTTTGT1986382.10753770614322No Hit
AAACCGTTACCATTACTGAGC1479811.5700698622256561No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1470351.5600328568691206No Hit
TTCAAGTAATCCAGGATAGGCT1392091.4769994489196No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1368571.4520448647773472No Hit
TCCTGTACTGAGCTGCCCCGAG1135881.2051621188563926No Hit
TGAGGTAGTAGGTTGTATAGT1011791.0735033456330856No Hit
TATTGCACTTGTCCCGGCCTGT857050.9093250994522936No Hit
TCCTGTACTGAGCTGCCCCGA854480.9065983442972939No Hit
TGAGGTAGTAGGTTGTATAGTT796590.8451773886852605No Hit
TGAGGTAGTAGTTTGTGCTGTT753730.7997031762559679No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT748330.7939738074477974No Hit
TGAGGGGCAGAGAGCGAGACTTT716800.7605206595734252No Hit
TGAGGTAGTAGATTGTATAGT691300.7334653068681763No Hit
TGAGGTAGTAGATTGTATAGTT626720.664946299899354No Hit
AAGCTGCCAGTTGAAGAACTGT600020.6366177541256229No Hit
TAGCTTATCAGACTGATGTTGAC593380.629572752480021No Hit
CGCGACCTCAGATCAGACGT549150.582644977964211No Hit
TGAGGTAGTAGTTTGTACAGT546510.57984395321355No Hit
TGAGGGGCAGAGAGCGAGACT508840.5398763008054431No Hit
AAACCGTTACCATTACTGAG469750.49840203659963234No Hit
TGAGGTAGTAGGTTGTGTGGT444240.4713360739521462No Hit
CGCGACCTCAGATC426700.45272623526782996No Hit
CGCGACCTCAGATCAGA417620.44309240771631386No Hit
TCAGTGCACTACAGAACTTTGC416550.44195714389691726No Hit
CCCATAAAGTAGAAAGCACT407100.4319307484826191No Hit
TCCTGTACTGAGCTGCCCCGAGA385220.4087161948672919No Hit
TGAGGTAGTAGGTTGTGTGGTT368130.3905838035836566No Hit
CATTGCACTTGTCTCGGTCTGA338690.35934813363689083No Hit
TTCAAGTAATCCAGGATAGGCC334300.35469036899469314No Hit
TCCTGTACTGAGCTGCCCCGAGT311460.3304572609245801No Hit
TCCTGTACTGAGCTGCCCCG303540.3220541866725969No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC296920.3150304049114696No Hit
TAGCTTATCAGACTGATGTTGA296360.3144362481461778No Hit
TGGCTCAGTTCAGCAGGAAC279390.2964311761693907No Hit
TGAGGTAGTAGTTTGTACAGTT269300.2857257444519021No Hit
AAAAGCTGGGTTGAGAGGGCGA260000.27585849817116426No Hit
TTCAAGTAATTCAGGATAGGT250990.2662989402153097No Hit
AAACCGTTACCATTACTGAGTT242620.2574184185626457No Hit
TTCACAGTGGCTAAGTTCCG242140.2569091413352527No Hit
TGTAAACATCCCCGACTGGAAGCT238570.25312139195651795No Hit
CGCGACCTCAGATCAGAC211940.22486711577844828No Hit
TATTGCACTTGTCCCGGCCTGC211880.22480345612502414No Hit
TCGTACCGTGAGTAATAATGCG210890.22375307184352627No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT207630.22029423067414935No Hit
TGAGGGGCAGAGAGCGAGACTT201890.21420412382990905No Hit
TGAGGTAGTAGGTTGTATAGC201860.21417229400319698No Hit
GGCTGGTCCGATGGTAGT176710.18748828927625552No Hit
AGAGGTAGTAGGTTGCATAGT176480.18724426060479643No Hit
TGAGGTAGTAGTTTGTGCTGT175730.18644851493699496No Hit
TGAGGTAGTAGGTTGTATAG175530.18623631609224792No Hit
GGCTGGTCCGATGGTAGTGGGT171360.18181197017927195No Hit
TGAGGTAGTAGGTTGTATAGTC169840.18019925895919436No Hit
TGAGAACTGAATTCCATGGGTT169110.17942473317586763No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC168410.17868203721925296No Hit
CAACGGAATCCCAAAAGCAGCT160350.1701304237759469No Hit
TAGCTTATCAGACTGATGTTG160240.170013714411336No Hit
TGTAAACATCCTTGACTGGAAGCT160170.16993944481567452No Hit
TGAGGTAGTAGTTTGTGCTGTC157570.16718085983396289No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC157100.16668219254880734No Hit
AGAGGTAGTAGGTTGCATAGTT148740.15781228083838067No Hit
TAGCTTATCAGACTGATGTTGAT147420.15641176846305013No Hit
TCCTGTACTGAGCTGCCCCGAA146010.15491576660758344No Hit
AAACCGTTACCATTACTG143560.15231633075943207No Hit
AGCTCGGTCTGAGGCCCCTCAGT141180.1497911645069422No Hit
GGCTGGTCCGATGGTAGTGGGTT140990.14958957560443248No Hit
TACAGTATAGATGATGTACT138770.14723416842774023No Hit
AAACCGTTACCATTACTGAGA138730.14719172865879085No Hit
TGAGGTAGTAGATTGTATAGC136010.14430582437023098No Hit
TGAGGTAGTAGATTGTATAGTC132400.14047563522254672No Hit
TGGAGAGAAAGGCAGTTCCTGA130910.1388947538291812No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT130480.13843852631297504No Hit
TGAGATGAAGCACTGTAGCT128810.13666666595933719No Hit
TTCAAGTAATCCAGGATAGGCA127150.13490541554793667No Hit
AAGCTGCCAGTTGAAGAACTGC125610.13327148444338438No Hit
CGCGACCTCAGATCAGACGC123040.1305447292883848No Hit
CATTGCACTTGTCTCGGTCTG123020.1305235094039101No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT122720.13020521113678954No Hit
TGAGGGGCAGAGAGCGAGACTTC120370.1277118747110117No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA120100.12742540627060317No Hit
CGCGACCTCAGATCAGACG120020.12734052673270435No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC117930.1251230488050977No Hit
GGCTGGTCCGATGGTAGTG116770.1238922955055648No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT115340.12237507376562341No Hit
AAACCGTTACCATTACTGAGG114180.12114432046609051No Hit
TGTAAACATCCCCGACTGGAAG112960.11984990751313351No Hit
TGAGGGGCAGAGAGCGAGACC112500.11936185017021529No Hit
GCATTGGTGGTTCAGTGGTAGAATT110380.11711254241589658No Hit
TGAGGTAGTAGTTTGTACAGC109110.11576507975175282No Hit
CAACGGAATCCCAAAAGCAGCTG105370.111796961354983No Hit
AAGCTGCCAGTTGAAGAACT105140.11155293268352388No Hit
CTAGACTGAAGCTCCTTGAGG102030.10825324064770726No Hit
TGAGGTAGTAGGTTGTAT99370.10543099601257151No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA99020.10505964803426418No Hit
TGAGGTAGTAGGTTGTGTGGC98990.10502781820755211No Hit
TGAGGTAGTAGATTGTATAG98890.10492171878517859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)