FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12364870
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11846709.580933725951022No Hit
TGGAGTGTGACAATGGTGTTT7875276.369068174594637No Hit
TCAGTGCACTACAGAACTTTGT4175343.3767763025409887No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3126932.5288822284423533No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2536592.0514489840976897No Hit
TTCAAGTAATCCAGGATAGGCT2332701.886554407769754No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2121971.7161280304604902No Hit
TATTGCACTTGTCCCGGCCTGT1674721.354417798165286No Hit
AAACCGTTACCATTACTGAGC1601381.2951045987543743No Hit
TGAGGTAGTAGGTTGTATAGT1489771.2048408111043625No Hit
AAGCTGCCAGTTGAAGAACTGT1287251.04105421245836No Hit
TGAGGTAGTAGGTTGTATAGTT1252411.0128776121382594No Hit
TAGCTTATCAGACTGATGTTGAC1160950.9389099925838282No Hit
TGAGGTAGTAGTTTGTGCTGTT972200.7862597827555001No Hit
TGAGGTAGTAGATTGTATAGT949940.7682571672811764No Hit
TGAGGTAGTAGATTGTATAGTT945060.7643105022535619No Hit
TGGAGTGTGACAATGGTGTTC922800.7463078867792383No Hit
TCCTGTACTGAGCTGCCCCGAG878040.7101085575505444No Hit
TGAGGGGCAGAGAGCGAGACTTT856740.6928823351964072No Hit
TAGCTTATCAGACTGATGTTGA839480.6789234338897215No Hit
TGAGGTAGTAGGTTGTGTGGT798180.645522354865033No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT767280.6205322013090312No Hit
CATTGCACTTGTCTCGGTCTGA676640.5472277508780926No Hit
TCCTGTACTGAGCTGCCCCGA656730.5311256810625586No Hit
TGGAGTGTGACAATGGTGTTTG638800.5166249220574094No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC615360.4976679900395233No Hit
CCCATAAAGTAGAAAGCACT611830.494813127837171No Hit
CGCGACCTCAGATCAGA602460.4872352074870177No Hit
TCAGTGCACTACAGAACTTTGC595640.48171958136235965No Hit
TGAGGTAGTAGGTTGTGTGGTT593640.4801020956953045No Hit
TCGTACCGTGAGTAATAATGCG592670.4793176151467827No Hit
TGAGGTAGTAGTTTGTACAGT580740.4696693131427989No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT567070.45861379860847706No Hit
TAGCTTATCAGACTGATGTTG559020.4521034187985802No Hit
AAACCGTTACCATTACTGAG528930.42776834693773574No Hit
AACCCGTAGATCCGATCTTGT493200.39887196549579573No Hit
TGAGGGGCAGAGAGCGAGACT466890.3775939415456855No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC418310.3383052146929163No Hit
TTCAAGTAATCCAGGATAGGCC412070.3332586594117043No Hit
TGGCTCAGTTCAGCAGGAAC382880.3096514561010346No Hit
TTCACAGTGGCTAAGTTCCG376960.30486369852655143No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC357010.2887292789976765No Hit
TGGAGTGTGACAATGGTGTT348580.2819115769110391No Hit
TGTAAACATCCCCGACTGGAAGCT322280.2606416403892641No Hit
TATTGCACTTGTCCCGGCCTGC313720.25371880173426814No Hit
TGAGGTAGTAGTTTGTACAGTT310960.25148667151373205No Hit
TCCTGTACTGAGCTGCCCCGAGT305920.24741060763275313No Hit
AAAAGCTGGGTTGAGAGGGCGA303070.24510569055719955No Hit
TGAGAACTGAATTCCATGGGTT299420.24215377921482395No Hit
CGCGACCTCAGATC290790.23517432856148104No Hit
TTCAAGTAATTCAGGATAGGT288810.23357301775109646No Hit
AAACCGTTACCATTACTGAGTT286440.2316562972356361No Hit
CGCGACCTCAGATCAGACGT284520.2301035109952632No Hit
TGAGGTAGTAGGTTGTATAG273560.22123968953980105No Hit
TGAGATGAAGCACTGTAGCT254960.20619707283618832No Hit
TCCTGTACTGAGCTGCCCCGAGA250490.2025819923703201No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC246580.19941980789122735No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT246140.19906396104447519No Hit
CTAGACTGAAGCTCCTTGAGG233430.18878483963033982No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT228070.18444997804263208No Hit
TCCTGTACTGAGCTGCCCCG222300.179783531893178No Hit
TGTAAACATCCCCGACTGGAAG216150.1748097634669835No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG215800.17452670347524882No Hit
TGTAAACATCCTTGACTGGAAGCT212070.171510092706191No Hit
TGAGGTAGTAGTTTGTGCTGT207510.1678222253853053No Hit
AAACCGTTACCATTACTG206430.16694878312509553No Hit
TGAGGTAGTAGGTTGTGTGG202680.16391599749936717No Hit
TGAGGTAGTAGGTTGTATAGC200530.1621772004072829No Hit
AGCTCGGTCTGAGGCCCCTCAGT195970.15848933308639718No Hit
TGGCTCAGTTCAGCAGGAACAG192420.15561829602737431No Hit
CATTGCACTTGTCTCGGTCTG191380.15477720348050567No Hit
AAGCTGCCAGTTGAAGAACTGC190290.1538956737919606No Hit
TGAGATGAAGCACTGTAGCTC190040.15369348808357872No Hit
TGAGGGGCAGAGAGCGAGACTT176750.14294529582599735No Hit
TGGAGTGTGACAATGGTGTTTGA175850.14221742727582254No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT175490.14192627985575262No Hit
GCATTGGTGGTTCAGTGGTAGAATT173790.14055141703875576No Hit
TCAGTGCATCACAGAACTTTGT171870.13899863079838282No Hit
CAACGGAATCCCAAAAGCAGCT170290.13772081712140927No Hit
TACCCTGTAGAACCGAATTTGT169690.13723557142129275No Hit
AAGCTGCCAGTTGAAGAACT164890.13335360582036043No Hit
TGAGGTAGTAGGTTGTATAGTC164540.1330705458286258No Hit
TGAGGTAGTAGGTTGTAT161860.13090311503477192No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT161520.13062814247137253No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA160820.13006202248790322No Hit
TAGCAGCACGTAAATATTGGCG153600.12422289922983419No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT152450.12329284497127749No Hit
TAGCTTATCAGACTGATGTTGAT152040.12296126040953119No Hit
AGAGGTAGTAGGTTGCATAGT149050.12054311933728377No Hit
TTCACAGTGGCTAAGTTCT145530.11769634456326673No Hit
TTATAAAGCAATGAGACTGATT140650.11374967953565222No Hit
AGGAAACTCTGGTG139880.11312694755383598No Hit
TACAGTATAGATGATGTACT139690.11297328641546577No Hit
TGAGGTAGTAGATTGTATAG139390.11273066356540748No Hit
AGAGGTAGTAGGTTGCATAGTT138840.11228585500696732No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT138520.1120270573002385No Hit
TGAGGTAGTAGTTTGTGCTGTC138270.1118248715918566No Hit
TGAGGTAGTAGATTGTAT137950.11156607388512778No Hit
AAAAGCTGGGTTGAGAGGGCGT134670.10891339739115738No Hit
TGAGGTAGTAGGTTGTGTGGC132270.10697241459069122No Hit
TGTAAACATCCCCGACTGGAAGC131470.10632542032386916No Hit
CAACGGAATCCCAAAAGCAGCTG130030.10516083064358946No Hit
TGAGGTAGTAGATTGTATAGC126560.1023544930112488No Hit
TGGAGTGTGACAATGGTGT126320.10216039473120218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)