FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11159094
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8244387.388037057488717No Hit
TCAGTGCACTACAGAACTTTGT2771952.4840278251979955No Hit
TGGAGTGTGACAATGGTGTTT2409382.1591179355600016No Hit
TTCAAGTAATCCAGGATAGGCT2357272.11242059615234No Hit
TATTGCACTTGTCCCGGCCTGT1659081.486751523017908No Hit
TGAGGTAGTAGGTTGTATAGT1532451.3732745687060257No Hit
AAACCGTTACCATTACTGAGC1392551.247905967993459No Hit
TGAGGTAGTAGGTTGTATAGTT1345531.2057699307847034No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1296461.1617968268750132No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1240451.1116045800850858No Hit
TGAGGTAGTAGATTGTATAGTT1108720.9935573622733171No Hit
TAGCTTATCAGACTGATGTTGAC1036110.9284893558563088No Hit
TGAGGTAGTAGATTGTATAGT1031610.9244567704152327No Hit
CGCGACCTCAGATCAGA1027950.9211769342564907No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1023350.9170547358056129No Hit
AAGCTGCCAGTTGAAGAACTGT1012190.907053923911744No Hit
TGAGGTAGTAGTTTGTGCTGTT783690.7022881965148784No Hit
TCCTGTACTGAGCTGCCCCGAG770780.6907191569494799No Hit
CGCGACCTCAGATC675020.6049057387633799No Hit
TAGCTTATCAGACTGATGTTGA635900.5698491293289581No Hit
TCCTGTACTGAGCTGCCCCGA608210.5450352869148696No Hit
TGAGGTAGTAGTTTGTACAGT600880.5384666532964056No Hit
CATTGCACTTGTCTCGGTCTGA562260.5038581089109923No Hit
TGAGGGGCAGAGAGCGAGACTTT557520.49961045224639206No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT544330.48779049625354887No Hit
CCCATAAAGTAGAAAGCACT541080.4848780734349939No Hit
TGAGGTAGTAGGTTGTGTGGT540680.4845196213957872No Hit
TTCAAGTAATCCAGGATAGGCC507590.4548666764524073No Hit
TCAGTGCACTACAGAACTTTGC496740.4451436648889238No Hit
CGCGACCTCAGATCAGACGT487440.4368096549773664No Hit
TGAGGTAGTAGGTTGTGTGGTT451210.40434286152621357No Hit
TCGTACCGTGAGTAATAATGCG442110.39618807763425956No Hit
TGAGGTAGTAGTTTGTACAGTT414820.3717326872593779No Hit
AAACCGTTACCATTACTGAG402720.36088951307337314No Hit
TATTGCACTTGTCCCGGCCTGC379210.33982149446899546No Hit
TGGAGTGTGACAATGGTGTTC377650.338423531516089No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC345310.3094426841462219No Hit
TGTAAACATCCCCGACTGGAAGCT341460.30599258326885675No Hit
TAGCTTATCAGACTGATGTTG332110.2976137668523986No Hit
AACCCGTAGATCCGATCTTGT314180.28154615419495527No Hit
TGAGGGGCAGAGAGCGAGACT292460.2620822084660278No Hit
TCCTGTACTGAGCTGCCCCGAGT285960.2562573628289178No Hit
TTCAAGTAATTCAGGATAGGT282320.25299544927213624No Hit
TGAGAACTGAATTCCATGGGTT281930.25264595853390964No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT281920.2526369972329295No Hit
TGGCTCAGTTCAGCAGGAAC271160.24299463737826746No Hit
AAACCGTTACCATTACTGAGTT264680.2371877143431178No Hit
TGAGGTAGTAGGTTGTATAGC258130.231318062201107No Hit
AAAAGCTGGGTTGAGAGGGCGA251310.22520645493263164No Hit
CAACGGAATCCCAAAAGCAGCT246970.22131725030723823No Hit
TGGAGTGTGACAATGGTGTTTG240220.215268372145624No Hit
TGAGGTAGTAGGTTGTATAG238080.2133506537358678No Hit
TGAGGTAGTAGGTTGTATAGTC235310.21086837336436096No Hit
TCCTGTACTGAGCTGCCCCGAGA235010.21059953433495587No Hit
TTCACAGTGGCTAAGTTCCG234200.20987366895556217No Hit
TAGCTTATCAGACTGATGTTGAT233600.209335990896752No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC228500.20476572739686572No Hit
TGAGATGAAGCACTGTAGCT207170.18565127240616489No Hit
GCATTGGTGGTTCAGTGGTAGAATT200180.1793873230210266No Hit
AGCTCGGTCTGAGGCCCCTCAGT194370.17418080715154832No Hit
TCCTGTACTGAGCTGCCCCG192500.17250504386825669No Hit
TGAGGTAGTAGATTGTATAGTC190780.17096370009966758No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT188810.16919832380657426No Hit
AAGCTGCCAGTTGAAGAACTGC188260.16870545225266498No Hit
AGAGGTAGTAGGTTGCATAGTT181350.16251319327536806No Hit
AGAGGTAGTAGGTTGCATAGT179610.1609539269048186No Hit
TGAGGTAGTAGTTTGTGCTGT168920.15137429615701775No Hit
TGAGGTAGTAGATTGTATAGC167400.15001217840803205No Hit
TGTAAACATCCCCGACTGGAAG167000.1496537263688253No Hit
TGTAAACATCCTTGACTGGAAGCT164280.14721625250221926No Hit
TGAGGTAGTAGGTTGTAT161770.1449669659561968No Hit
CTAGACTGAAGCTCCTTGAGG161230.14448305570326767No Hit
TTCAAGTAATCCAGGATAGGCA160240.1435958869062309No Hit
CAACGGAATCCCAAAAGCAGCTG156240.14001136651416324No Hit
TCAGTGCATCACAGAACTTTGT155740.13956330146515478No Hit
TACCCTGTAGAACCGAATTTGT148300.13289609353590892No Hit
CATTGCACTTGTCTCGGTCTG148060.13268102231238485No Hit
TGAGGTAGTAGATTGTAT146850.1315967048937844No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT145860.13070953609674765No Hit
TGAGGGGCAGAGAGCGAGACTT145070.1300015933193143No Hit
TGAGGTAGTAGTTTGTGCTGTC143740.12880974028895179No Hit
GGCTGGTCCGATGGTAGT142090.12733112562722385No Hit
GGGTGCTGTAGGCTT137550.12326269498222704No Hit
AAACCGTTACCATTACTG135920.12180200292245948No Hit
TGAGGTAGTAGATTGTATAG134110.12018000744504886No Hit
AAGCTGCCAGTTGAAGAACT128300.11497349157557056No Hit
TGAGATGAAGCACTGTAGCTC123880.11101259654233578No Hit
TGGCTCAGTTCAGCAGGAACAG122840.11008062124039819No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC120400.10789406380123691No Hit
TGTAAACATCCCCGACTGGAAGC117820.10558204814835326No Hit
GGCTGGTCCGATGGTAGTGGGT117030.10487410537091989No Hit
TTCAAGTAATTCAGGATAGGTT115730.10370913624349791No Hit
AAAAGCTGGGTTGAGAGGGCGAT115240.10327003249546961No Hit
TTATAAAGCAATGAGACTGATT112300.10063541000729988No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC112030.10039345488083531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)