FastQCFastQC Report
Wed 8 May 2024
EGAF00002167671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11202625
Total Bases253.6 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8617197.692116802981444No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3685203.2895861461041496No Hit
TCAGTGCACTACAGAACTTTGT2813652.5115988440209325No Hit
TCCTGTACTGAGCTGCCCCGAG2704652.4143002198145522No Hit
TGAGGTAGTAGGTTGTATAGT1970741.759176978609924No Hit
TCCTGTACTGAGCTGCCCCGA1738801.552136218073889No Hit
CGCGACCTCAGATCAGA1590741.4199707657803418No Hit
TGAGGTAGTAGGTTGTATAGTT1385521.2367815578937973No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1199111.070383057542317No Hit
TGAGGTAGTAGATTGTATAGT1128441.0072996284352997No Hit
TGAGGTAGTAGGTTGTGTGGT1118850.9987391348009953No Hit
TGAGGTAGTAGGTTGTGTGGTT952220.8499972104752235No Hit
TTCAAGTAATCCAGGATAGGCT922450.8234230816438112No Hit
TATTGCACTTGTCCCGGCCTGT902310.8054451523638434No Hit
TCCTGTACTGAGCTGCCCCGAGA854430.7627051695473159No Hit
TGAGGTAGTAGATTGTATAGTT853310.7617054038673972No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT815030.7275348411644592No Hit
TGAGGTAGTAGTTTGTGCTGTT790660.705781011146941No Hit
TGAGGTAGTAGGTTGTATAG773560.6905167315696098No Hit
TAGCTTATCAGACTGATGTTGAC759310.6777964985885004No Hit
AAACCGTTACCATTACTGAGC750430.6698697849834302No Hit
CATTGCACTTGTCTCGGTCTGA744850.6648888095424064No Hit
TGAGGGGCAGAGAGCGAGACTTT697990.6230593276129478No Hit
AAACCGTTACCATTACTGAG689930.6158645853092467No Hit
TCCTGTACTGAGCTGCCCCG596240.5322324008881847No Hit
TGAGGGGCAGAGAGCGAGACT595470.5315450619832405No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC589740.5264301893529418No Hit
TGAGGTAGTAGTTTGTACAGT584600.5218419790004575No Hit
TAGCTTATCAGACTGATGTTGA574750.5130493969047434No Hit
TCGTACCGTGAGTAATAATGCG567220.5063277580031467No Hit
AAAAGCTGGGTTGAGAGGGCGA548290.4894299327166624No Hit
AAGCTGCCAGTTGAAGAACTGT545950.487341136563975No Hit
TGAGGTAGTAGGTTGTGTGG440820.3934970598408855No Hit
AAATGGATTTTTGGAGCAGGGAGA428710.3826870934267638No Hit
TGGAGTGTGACAATGGTGTTT406060.3624686178462637No Hit
TTCACAGTGGCTAAGTTCCG398920.3560951116367816No Hit
CGCGACCTCAGATC397550.3548721839747381No Hit
TGGCTCAGTTCAGCAGGAAC388120.3464545140089934No Hit
AAATGGATTTTTGGAAATAGGAGA365910.32662880351703283No Hit
CGCGACCTCAGATCAGACGT362760.3238169625422613No Hit
CCCATAAAGTAGAAAGCACT355620.31744345633277915No Hit
TGAGGTAGTAGATTGTATAG351200.313497952488814No Hit
TCCTGTACTGAGCTGCCCCGAGT326440.2913959897791812No Hit
AAACCGTTACCATTACTG325860.29087825398065187No Hit
TGAGGTAGTAGTTTGTACAGTT305990.2731413396413787No Hit
CTAGACTGAAGCTCCTTGAGG304160.2715077939322257No Hit
TAGCTTATCAGACTGATGTTG300640.2683656732239096No Hit
AAATGGATTTTTGGAGCAGGGAG292200.26083172470737886No Hit
CATTGCACTTGTCTCGGTCTG280710.25057520000892647No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC262490.23431115475167652No Hit
TCAGTGCACTACAGAACTTTGC260750.23275794735608843No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA257930.2302406801977215No Hit
TGGCTCAGTTCAGCAGGAACAG248220.2215730688119972No Hit
AAATGGATTTTTGGAGCAGGGA241680.2157351513596144No Hit
TGTAAACATCCCCGACTGGAAG228980.20439852266767833No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT220410.19674852992044276No Hit
TTCAAGTAATTCAGGATAGGT216590.19333861483357695No Hit
TGTAAACATCCCCGACTGGAAGCT209650.18714363820979457No Hit
TATTGCACTTGTCCCGGCCTG201670.18002030774037336No Hit
AAATGGATTTTTGGAAATAGGAG199990.17852065922049518No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC199340.1779404380669709No Hit
CGTGTATTTGACAAGCTGAGTTG197310.17612836277211816No Hit
TGGAGAGAAAGGCAGTTCCTGA192200.17156693185748875No Hit
TGAGGGGCAGAGAGCGAGACTT188620.16837125227346267No Hit
TGTAAACATCCCCGACTGGAAGC185660.16572901440510596No Hit
TGAGGTAGTAGGTTGTAT185240.16535410227513642No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA183490.16379196840026333No Hit
AAAAGCTGGGTTGAGAGGGCG177330.1582932571607101No Hit
TGAGGTAGTAGGTTGTATAGC176580.15762377121433593No Hit
TGAGGTAGTAGTTTGTGCTGT176070.15716852077080148No Hit
AAATGGATTTTTGGAGCAGGG175220.15640977003157744No Hit
AGAGGTAGTAGGTTGCATAGT175100.15630265228015755No Hit
CAACGGAATCCCAAAAGCAGCT170570.15225895716405755No Hit
TGAGATGAAGCACTGTAGCTC169750.15152698586268842No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT164140.14651923098380962No Hit
AAACCGTTACCATTACTGAGTT151560.13528972004329343No Hit
AAGCTGCCAGTTGAAGAACT139640.12464935673558653No Hit
AAAAGCTGGGTTGAGAGGGCGAA137340.12259626650003906No Hit
GGCTGGTCCGATGGTAGTGGGTTATC137090.12237310451791432No Hit
CAACGGAATCCCAAAAGCAGCTG136550.12189107463652493No Hit
TGAGGTAGTAGATTGTAT134570.12012363173809709No Hit
AGAGGTAGTAGGTTGCATAGTT132320.11811517389897458No Hit
TGGAGAGAAAGGCAGTTCCTG131840.1176867028932951No Hit
TGGCTCAGTTCAGCAGGAACA131760.11761529105901519No Hit
TGAGAACTGAATTCCATGGGTT129420.11552649490632777No Hit
CGCGACCTCAGATCAGAC128510.11471418529139375No Hit
TGAGATGAAGCACTGTAGCT128360.11458028810211891No Hit
AACATTCAACGCTGTCGGTGA127610.11391080215574474No Hit
TGTAAACATCCTTGACTGGAAGCT127160.11350911058792025No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA126430.11285747760011605No Hit
TAGCAGCACGTAAATATTGGC124440.11108110822240322No Hit
CGCGACCTCAGATCAGACG119390.1065732361834838No Hit
TGAGGTAGTAGTTTGTGCT119320.10651075082848886No Hit
AGCTCGGTCTGAGGCCCCTCAGT119020.10624295644993918No Hit
TGAGGTAGTAGTTTGTGCTG117530.10491291103647583No Hit
TATTGCACTTGTCCCGGCCTGTA115150.10278840896664845No Hit
CTAGACTGAAGCTCCTTGAGGA114840.10251168810881378No Hit
TACAGTACTGTGATAACTGAAG114610.10230637908525902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)