FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11168751
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6901456.179249586636859No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2522822.2588201670894086No Hit
TCAGTGCACTACAGAACTTTGT1885641.6883176999827463No Hit
TCCTGTACTGAGCTGCCCCGAG1523181.3637872309983452No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1323681.1851638558331186No Hit
TTCAAGTAATCCAGGATAGGCT1316521.1787531121429782No Hit
TGAGGTAGTAGGTTGTATAGT1225311.0970877585148062No Hit
CGCGACCTCAGATCAGA1215291.0881162987696655No Hit
AAACCGTTACCATTACTGAGC1145801.0258980614752715No Hit
TATTGCACTTGTCCCGGCCTGT1055410.9449668991635681No Hit
TGAGGTAGTAGATTGTATAGT1028350.9207385857201043No Hit
TCCTGTACTGAGCTGCCCCGA973910.8719954451487011No Hit
CGCGACCTCAGATC845390.7569243866212076No Hit
TGAGGTAGTAGATTGTATAGTT824050.7378175052877444No Hit
TGAGGTAGTAGGTTGTATAGTT824000.7377727375245451No Hit
TGAGGTAGTAGTTTGTGCTGTT766370.6861734136610261No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT741110.6635567396927373No Hit
TGAGGTAGTAGTTTGTACAGT713300.638656909801284No Hit
AAACCGTTACCATTACTGAG674110.6035679370056688No Hit
TAGCTTATCAGACTGATGTTGAC629270.5634202069685321No Hit
TAGCTTATCAGACTGATGTTGA574580.5144532275811323No Hit
CGCGACCTCAGATCAGACGT554780.4967251933542077No Hit
AAGCTGCCAGTTGAAGAACTGT552460.49464796914175985No Hit
CATTGCACTTGTCTCGGTCTGA458930.4109053913011401No Hit
CCCATAAAGTAGAAAGCACT443770.3973318054991109No Hit
TGAGGTAGTAGGTTGTGTGGT410290.3673553112608563No Hit
TGAGGGGCAGAGAGCGAGACTTT404470.3621443436244572No Hit
TCCTGTACTGAGCTGCCCCGAGA402490.36037154020176476No Hit
TCCTGTACTGAGCTGCCCCG348250.3118074706831587No Hit
TGAGGTAGTAGGTTGTGTGGTT346360.310115249234225No Hit
AAACCGTTACCATTACTGAGG343770.307796279100501No Hit
TGAGGTAGTAGTTTGTACAGTT336400.3011975108049235No Hit
TCAGTGCACTACAGAACTTTGC333700.29878005159216103No Hit
TGAGGTAGTAGGTTGTATAG292010.2614526906365806No Hit
TTCAAGTAATTCAGGATAGGT289740.2594202341873321No Hit
TTCAAGTAATCCAGGATAGGCC274420.24570339154306509No Hit
TGTAAACATCCCCGACTGGAAGCT273410.24479908272643916No Hit
TAGCTTATCAGACTGATGTTG268670.24055509877514503No Hit
TGAGGGGCAGAGAGCGAGACT260700.23341911732117585No Hit
AAAAGCTGGGTTGAGAGGGCGA258070.2310643329768924No Hit
TGGCTCAGTTCAGCAGGAAC251500.22518184889250373No Hit
CAACGGAATCCCAAAAGCAGCT249560.2234448596803707No Hit
TCCTGTACTGAGCTGCCCCGAGT247490.22159147428391948No Hit
TTCACAGTGGCTAAGTTCCG246420.22063344415145436No Hit
TCGTACCGTGAGTAATAATGCG246280.22050809441449631No Hit
AAACCGTTACCATTACTGAGTT238780.21379292993460056No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT235060.2104622083525723No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC234760.21019360177337645No Hit
TATTGCACTTGTCCCGGCCTGC228830.2048841450579389No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC223700.20029097255369022No Hit
TGAGGTAGTAGTTTGTGCTGT221340.198177934130683No Hit
TGAGAACTGAATTCCATGGGTT216120.19350417965267558No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT213770.19140009478230824No Hit
TGAGGTAGTAGATTGTATAG203280.18200781806309407No Hit
TGAGGTAGTAGGTTGTATAGC202410.18122885898342617No Hit
AAATGGATTTTTGGAAATAGGAGA202400.1812199054307863No Hit
TGTAAACATCCTTGACTGGAAGCT197430.1767699897687754No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT194460.1741107846347367No Hit
AAATGGATTTTTGGAAATAGGAG189690.169839940025523No Hit
AAATGGATTTTTGGAGCAGGGA185810.16636596160125694No Hit
CGCGACCTCAGATCAGAC184350.16505874291583722No Hit
TGTAAACATCCCCGACTGGAAG177990.15936428343688566No Hit
AGAGGTAGTAGGTTGCATAGT177880.15926579435784718No Hit
CATTGCACTTGTCTCGGTCTG175910.15750194448779456No Hit
TGGAGAGAAAGGCAGTTCCTGA174000.15579181593358113No Hit
AAATGGATTTTTGGAGCAGGGAG171620.15366087040529422No Hit
AAATGGATTTTTGGAGCAGGGAGA168420.1507957335605387No Hit
TGAGGTAGTAGATTGTATAGC166910.14944374711191968No Hit
AAACCGTTACCATTACTG160910.1440716155280031No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT146370.1310531499896452No Hit
GGCTGGTCCGATGGTAGT146160.13086512538420814No Hit
TGAGATGAAGCACTGTAGCT138040.12359484064064102No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT137950.12351425866688227No Hit
TGAGGTAGTAGGTTGTATAGTC132570.11869724734663706No Hit
TGAGGTAGTAGATTGTATAGTC129080.11557245747532557No Hit
AAATGGATTTTTGGAGCAGGG128200.11478454484301781No Hit
AGCTCGGTCTGAGGCCCCTCAGT128180.1147666377377381No Hit
TGAGGTAGTAGGTTGTAT128080.11467710221133949No Hit
TGAGGTAGTAGTTTGTGCTGTC127830.11445326339534294No Hit
AAATGGATTTTTGGAAATAGGA127620.11426523878990587No Hit
TGAGGTAGTAGATTGTAT127590.11423837813198628No Hit
TACAGTACTGTGATAACTGAAG127470.11413093550030795No Hit
TATTGCACTTGTCCCGGCCTG126780.11351314036815756No Hit
TACAGTATAGATGATGTACT124320.11131056641875176No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA122860.11000334773333205No Hit
AAGCTGCCAGTTGAAGAACT122540.1097168340488565No Hit
TCCTGTACTGAGCTGCCCCGAA122290.10949299523285998No Hit
AGAGGTAGTAGGTTGCATAGTT120040.10747844588889124No Hit
AGCAGCATTGTACAGGGCTATG119000.1065472764143457No Hit
TGAGGTAGTAGTTTGTACAGC117620.10531168615004488No Hit
CGCGACCTCAGATCAGACG116160.10400446746462517No Hit
CAACGGAATCCCAAAAGCAGCTG114480.10250027062112853No Hit
TGAGGGGCAGAGAGCGAGACTT114060.10212422141025437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)