FastQCFastQC Report
Wed 8 May 2024
EGAF00002167673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11872261
Total Bases276.9 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9016757.594804393198566No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5104844.299804392777416No Hit
TCCTGTACTGAGCTGCCCCGAG2288621.9277035772714228No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1910631.6093227734801314No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1865441.5712592571878266No Hit
TCCTGTACTGAGCTGCCCCGA1611111.357037214731044No Hit
TCAGTGCACTACAGAACTTTGT1461381.2309197043427533No Hit
TATTGCACTTGTCCCGGCCTGT1419911.1959895423458093No Hit
AAACCGTTACCATTACTGAGC1315691.108205084103188No Hit
AAACCGTTACCATTACTGAG1147890.9668672209952258No Hit
TGAGGTAGTAGGTTGTATAGT1127590.949768540297421No Hit
TTCAAGTAATCCAGGATAGGCT877080.7387640820901764No Hit
TGAGGTAGTAGATTGTATAGT865820.7292797892499162No Hit
TAGCTTATCAGACTGATGTTGAC830090.6991844266226964No Hit
TGAGGTAGTAGGTTGTATAGTT783630.6600511899123511No Hit
CATTGCACTTGTCTCGGTCTGA731600.6162263447543816No Hit
TGAGGTAGTAGTTTGTGCTGTT683110.575383239974256No Hit
TGAGGTAGTAGATTGTATAGTT682780.5751052811254739No Hit
TAGCTTATCAGACTGATGTTGA680170.5729068793214704No Hit
TCCTGTACTGAGCTGCCCCGAGA667340.5621001762006411No Hit
CGCGACCTCAGATCAGA629580.5302949455036409No Hit
AAGCTGCCAGTTGAAGAACTGT624790.5262603306985923No Hit
TGAGGTAGTAGTTTGTACAGT608560.5125898091357661No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT579130.48780093362165805No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC547690.4613190360286048No Hit
CGCGACCTCAGATC535620.451152480559516No Hit
TCCTGTACTGAGCTGCCCCG530450.44679779192859725No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC511460.43080252363050303No Hit
TGAGGTAGTAGGTTGTATAG483780.40748767231448163No Hit
TGAGGTAGTAGGTTGTGTGGT472020.39758222970333956No Hit
TGAGGTAGTAGGTTGTGTGGTT386780.32578461676339493No Hit
TGAGGGGCAGAGAGCGAGACTTT384510.32387259680359115No Hit
CCCATAAAGTAGAAAGCACT373430.31453991788084845No Hit
AAAAGCTGGGTTGAGAGGGCGA372460.3137228873253376No Hit
TAGCTTATCAGACTGATGTTG333310.2807468602652856No Hit
AAACCGTTACCATTACTGAGTT328750.27690597435484277No Hit
CATTGCACTTGTCTCGGTCTG327830.2761310587764201No Hit
TGAGGGGCAGAGAGCGAGACT326530.27503606937212716No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT322910.2719869450309423No Hit
TCGTACCGTGAGTAATAATGCG306950.25854384434439237No Hit
TGAGGTAGTAGATTGTATAG296510.2497502371283785No Hit
AAACCGTTACCATTACTG286630.24142831765575234No Hit
TCCTGTACTGAGCTGCCCCGAGT271040.22829686779965502No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC270320.22769041212958507No Hit
TGTAAACATCCCCGACTGGAAG263160.22165954741055643No Hit
CGCGACCTCAGATCAGACGT259370.21846723214727168No Hit
TGTAAACATCCCCGACTGGAAGCT249040.20976627788085184No Hit
TGAGGTAGTAGTTTGTACAGTT249010.2097410088945989No Hit
TGAGGTAGTAGTTTGTGCTGT248750.20952201101374035No Hit
TATTGCACTTGTCCCGGCCTGC245670.20692772842510787No Hit
TGGCTCAGTTCAGCAGGAAC233840.19696332484604237No Hit
TCAGTGCACTACAGAACTTTGC228580.1925328292563649No Hit
TATTGCACTTGTCCCGGCCTG221120.1862492746748071No Hit
GCATTGGTGGTTCAGTGGTAGAATT216400.18227362083768206No Hit
TTCAAGTAATTCAGGATAGGT212660.17912342055148553No Hit
TACAGTACTGTGATAACTGAAG209810.17672286685745875No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT205200.1728398659699277No Hit
TTCACAGTGGCTAAGTTCCG204840.17253663813489276No Hit
TGGAGAGAAAGGCAGTTCCTGA203080.1710541909413885No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT200330.1687378672015381No Hit
TGTAAACATCCTTGACTGGAAGCT193130.16267331050083889No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA189240.1593967652833778No Hit
TGAGGTAGTAGGTTGTGTGG184110.15507576863412958No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT181550.15291948180721432No Hit
TAGCAGCACGTAAATATTGGCG180550.15207718226545053No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT176210.14842160225419573No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC175680.14797518349706093No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG171960.14484182920169966No Hit
TGAGGTAGTAGGTTGTATAGC166200.1399901838411403No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC165830.1396785330106877No Hit
CTAGACTGAAGCTCCTTGAGG158890.13383297419084705No Hit
TGAAAAGAACTTTGAAGAGAG157990.13307490460325966No Hit
TTCAAGTAATCCAGGATAGGCC155190.13071646588632108No Hit
TGAGGTAGTAGTTTGTGCTG155170.13069961989548579No Hit
TGAGAACTGAATTCCATGGGTT151110.12727988375592483No Hit
TGGAGAGAAAGGCAGTTCCTG146790.12364114973550532No Hit
CAACGGAATCCCAAAAGCAGCTG146700.12356534277674658No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC146490.12338845987297617No Hit
GGCTGGTCCGATGGTAGTG144060.1213416719864902No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG144060.1213416719864902No Hit
TGTAAACATCCCCGACTGGAAGC141930.11954757396253334No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA141360.11906746322372798No Hit
GGCTGGTCCGATGGTAGTGGGT140940.11871369741618719No Hit
GGAATACCGGGTGCTGTAGGCTT137920.1161699528000606No Hit
CGCGACCTCAGATCAGAC136780.11520973132244988No Hit
CAACGGAATCCCAAAAGCAGCT136240.11475488956989743No Hit
AAGCTGCCAGTTGAAGAACT133710.11262387172923506No Hit
TGAGATGAAGCACTGTAGCTC132900.11194160910040639No Hit
AGAGGTAGTAGGTTGCATAGT132030.11120880849907192No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA131620.11086346568694877No Hit
AGCAGCATTGTACAGGGCTATG126460.10651720005144766No Hit
GGCTGGTCCGATGGTAGT126460.10651720005144766No Hit
TGAGGTAGTAGATTGTATAGC126020.10614658825307159No Hit
AAAAGCTGGGTTGAGAGGGCG122650.10330803879732765No Hit
AAACCGTTACCATTACTGAGG120730.10169082367714119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)