FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16780014
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT190265311.338804604096278No Hit
TCAGTGCACTACAGAACTTTGT7103644.2333933690400976No Hit
AAACCGTTACCATTACTGAGC4012512.3912435353152866No Hit
TGAGGTAGTAGGTTGTATAGT2175621.2965543413730167No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2112711.2590633118661285No Hit
CGCGACCTCAGATCAGA1867021.1126450788420081No Hit
TTCAAGTAATCCAGGATAGGCT1809491.0783602445146947No Hit
TATTGCACTTGTCCCGGCCTGT1642160.9786404230652014No Hit
TCAGTGCACTACAGAACTTTGC1561010.930279319194847No Hit
TGAGGGGCAGAGAGCGAGACTTT1529250.9113520405882856No Hit
TGAGGTAGTAGGTTGTGTGGT1527310.9101959032930484No Hit
TGAGGTAGTAGATTGTATAGT1501880.8950409695724927No Hit
TGGAGTGTGACAATGGTGTTT1303930.7770732491641544No Hit
TCCTGTACTGAGCTGCCCCGAG1266360.7546835181424759No Hit
TGAGGTAGTAGGTTGTATAGTT1225120.7301066614127975No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1163960.69365853925986No Hit
AAGCTGCCAGTTGAAGAACTGT1134010.6758099248308136No Hit
TCCTGTACTGAGCTGCCCCGA1125000.6704404418256147No Hit
TGAGGTAGTAGTTTGTGCTGTT1116060.6651126751145738No Hit
AAACCGTTACCATTACTGAG1053930.6280864843140179No Hit
TGAGGTAGTAGGTTGTGTGGTT1048000.6245525182517726No Hit
TGAGGTAGTAGTTTGTACAGT997130.5942366913400668No Hit
TGAGGTAGTAGATTGTATAGTT985670.5874071380393366No Hit
TGAGGGGCAGAGAGCGAGACT976910.5821866417989878No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT969250.5776216873239797No Hit
CCCATAAAGTAGAAAGCACT954670.5689327791979196No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC869780.5183428333254072No Hit
TAGCTTATCAGACTGATGTTGA849810.5064417705491783No Hit
TAGCTTATCAGACTGATGTTGAC789490.47049424392613737No Hit
CATTGCACTTGTCTCGGTCTGA711940.424278549469625No Hit
TAGCTTATCAGACTGATGTTG706460.42101275958411No Hit
TTCACAGTGGCTAAGTTCCG698250.4161200342264315No Hit
TGGCTCAGTTCAGCAGGAAC640980.3819901461345622No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT581400.3464836203354777No Hit
TGAGATGAAGCACTGTAGCT554880.3306791043201752No Hit
CGCGACCTCAGATCAGACGT514560.30665051888514516No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG483060.2878781865140279No Hit
TGAGGTAGTAGGTTGTATAGC468350.2791118052702459No Hit
TGAGGTAGTAGGTTGTATAG466900.2782476820341151No Hit
AAATGGATTTTTGGAGCAGGGAGA453970.27054208655606604No Hit
TTCAAGTAATCCAGGATAGGCC446840.26629298402254015No Hit
TATTGCACTTGTCCCGGCCTGC431050.2568829799546055No Hit
TGAGGTAGTAGGTTGTGTGG421880.2514181454199025No Hit
AAACCGTTACCATTACTGAGTT406370.24217500652860005No Hit
AAACCGTTACCATTACTGAGA405980.24194258717543382No Hit
TCGTACCGTGAGTAATAATGCG392180.23371851775570626No Hit
TGAGATGAAGCACTGTAGCTC386430.23029182216415311No Hit
TCCTGTACTGAGCTGCCCCG367390.21894499015316674No Hit
TGAGGTAGTAGGTTGTGTGGC359090.21399862956014223No Hit
TCCTGTACTGAGCTGCCCCGAGA349840.20848611926068714No Hit
AAATGGATTTTTGGAAATAGGAGA347980.20737765773020211No Hit
AAACCGTTACCATTACTG344590.20535739719883425No Hit
CGCGACCTCAGATC344150.205095180492698No Hit
TGAGGGGCAGAGAGCGAGACTT340510.20292593319648006No Hit
TTCAAGTAATTCAGGATAGGT336260.20039315819402773No Hit
TCCTGTACTGAGCTGCCCCGAGT329800.1965433401903002No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT328000.19547063548337923No Hit
TGAGGTAGTAGTTTGTGCTGT325520.19399268677606588No Hit
TGAGGTAGTAGATTGTATAGC315900.18825967606463262No Hit
TGTAAACATCCTTGACTGGAAGCT298280.17775908887799496No Hit
TGAGGTAGTAGTTTGTACAGTT292310.1742012849333737No Hit
TGAGAACTGAATTCCATGGGTT289960.17280080934378245No Hit
TGAGGTAGTAGGTTGTATAGTC282920.16860534204560257No Hit
TGAGGGGCAGAGAGCGAGACTTC282220.1681881791040222No Hit
AAAAGCTGGGTTGAGAGGGCGA273180.16280081768704127No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC273170.16279485821644726No Hit
CATTGCACTTGTCTCGGTCTG270900.161442058391608No Hit
TGAGGTAGTAGGTTGTAT270710.16132882845032193No Hit
TGGAGTGTGACAATGGTGTTC267310.15930260844836006No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC266130.1585993909182674No Hit
TGAGGTAGTAGATTGTATAG265010.1579319302117388No Hit
AAGCTGCCAGTTGAAGAACTGC262000.15613812956294315No Hit
TGAGGTAGTAGGTTGTGTGGTC258610.1541178690315753No Hit
TGAGGTAGTAGTTTGTGCTGTC254780.1518353917940712No Hit
AAACCGTTACCATTACTGAGG253150.15086399808724832No Hit
TGAGGTAGTAGATTGTAT241900.14415959366899217No Hit
TGTAAACATCCCCGACTGGAAGCT241390.1438556606686979No Hit
AAGCTGCCAGTTGAAGAACT239960.1430034563737551No Hit
TCAGTGCACTACAGAACTTTGA236620.1410129931953573No Hit
TGAGGGGCAGAGAGCGAGACC230990.1376578112509322No Hit
TGTAAACATCCCCGACTGGAAG230700.13748498660370603No Hit
TACAGTACTGTGATAACTGAAG225000.13408808836512295No Hit
TGAGGTAGTAGATTGTATAGTC222950.1328663968933518No Hit
TAGCTTATCAGACTGATGTTGAT219090.1305660412440657No Hit
TGAGGTAGTAGTTTGTACAGC216630.12910001147794037No Hit
CCCATAAAGTAGAAAGCACC214990.12812265830052347No Hit
AAATGGATTTTTGGAGCAGGGAGAT214200.12765186012359706No Hit
TACAGTATAGATGATGTACT213170.12703803465241448No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC201150.1198747509984199No Hit
AGACCGTTACCATTACTGAGT197360.11761611164329185No Hit
TCAGTGCATCACAGAACTTTGT193650.11540514805291581No Hit
AAGCTGCCAGTTGAAGAAC187070.11148381640206022No Hit
TCCTGTACTGAGCTGCCCCGAA184220.10978536728276865No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT181650.10825378334010924No Hit
TTCAAGTAATCCAGGATAGGCA177250.10563161627874684No Hit
GGCTGGTCCGATGGTAGTGGGTT176970.1054647511021147No Hit
TTCACAGTGGCTAAGTTCCA173620.10346832845312287No Hit
AGCTCGGTCTGAGGCCCCTCAGT173440.10336105798243077No Hit
AACCCGTAGATCCGATCTTGT172560.1028366245701583No Hit
CGTGTATTTGACAAGCTGAGTTG171070.10194866345165146No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC168090.10017274121463784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)