FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002167675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9824069
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT111428311.34237758305647No Hit
TCAGTGCACTACAGAACTTTGT3241093.299131958458354No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2694072.742315836747482No Hit
TCCTGTACTGAGCTGCCCCGAG1449791.475753071359739No Hit
AAACCGTTACCATTACTGAG1245931.2682423138518266No Hit
CGCGACCTCAGATCAGA1190781.2121046788250367No Hit
TCCTGTACTGAGCTGCCCCGA1101531.121256375540522No Hit
TGAGGTAGTAGGTTGTATAGT1065881.0849679496347187No Hit
TTCAAGTAATCCAGGATAGGCT984851.0024868514258196No Hit
AAACCGTTACCATTACTGAGC877930.8936521109532111No Hit
TGAGGGGCAGAGAGCGAGACTTT851780.8670338125678881No Hit
TGAGGTAGTAGGTTGTATAGTT809600.8240984463769543No Hit
TATTGCACTTGTCCCGGCCTGT757970.7715438480735426No Hit
TTCACAGTGGCTAAGTTCCG753700.7671973802301266No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT741480.7547585425143085No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC733710.7468493961107154No Hit
TGAGGTAGTAGGTTGTGTGGT730510.7435920899985535No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT673150.685204877938052No Hit
TGAGGTAGTAGATTGTATAGT652070.6637473739241856No Hit
TGAGGGGCAGAGAGCGAGACT616940.6279882602616085No Hit
TGGCTCAGTTCAGCAGGAAC610580.6215143643636868No Hit
TCGTACCGTGAGTAATAATGCG592960.6035788225835954No Hit
TAGCTTATCAGACTGATGTTGAC574770.5850630731522752No Hit
TGAGGTAGTAGGTTGTGTGGTT555140.5650815359704823No Hit
TGGAGTGTGACAATGGTGTTT520510.5298313763879305No Hit
AAGCTGCCAGTTGAAGAACTGT513620.522817989165182No Hit
TGAGGTAGTAGATTGTATAGTT507840.5169344800000896No Hit
CATTGCACTTGTCTCGGTCTGA491890.5006988448472827No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC479450.48803606733625343No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG478290.4868552938705948No Hit
TAGCTTATCAGACTGATGTTGA468620.4770121219629056No Hit
ACGGCCCTGGCGGAGC436370.44418458380127424No Hit
TGAGGTAGTAGTTTGTGCTGTT433180.44093745677071283No Hit
AAAAGCTGGGTTGAGAGGGCGA403800.4110313150284266No Hit
TGAGGTAGTAGGTTGTGTGG398890.4060333859625782No Hit
TGAGGTAGTAGGTTGTATAG392660.39969181812546306No Hit
TGGCTCAGTTCAGCAGGAACAG391680.3986942681286135No Hit
CCCATAAAGTAGAAAGCACT385600.3925053865155059No Hit
ACGGCCCTGGCGGA380360.3871715477568409No Hit
TCCTGTACTGAGCTGCCCCG372210.3788755962524286No Hit
TGAGGTAGTAGTTTGTACAGT358880.3653068804789543No Hit
CGCGACCTCAGATCAGACGT352850.3591688942738493No Hit
AAACCGTTACCATTACTG346700.3529087590895381No Hit
TCCTGTACTGAGCTGCCCCGAGA332320.33827123974801077No Hit
TAGCTTATCAGACTGATGTTG324560.3303722724260182No Hit
CGGTCGGGCTGGGGCG317500.32318584081606105No Hit
AAATGGATTTTTGGAGCAGGGAGA316570.322239186227214No Hit
AAACCGTTACCATTACTGAGTT275940.2808815776843587No Hit
AGGGCGTGAAACCGTTAAGAGG270720.2755680970888946No Hit
TCAGTGCACTACAGAACTTTGC268840.27365442974799953No Hit
CGGACAGGATTGACAGA239980.2442776002489396No Hit
CATTGCACTTGTCTCGGTCTG225020.22904969417458285No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC223650.22765515999531358No Hit
CTAGACTGAAGCTCCTTGAGG219460.2233901248047016No Hit
TGTAAACATCCCCGACTGGAAG212920.21673300543797075No Hit
TGAGGTAGTAGATTGTATAG203640.20728681771270135No Hit
CGGTCGGGCTGGGGCGC203230.20686947536708056No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA203090.20672696822467349No Hit
AAATGGATTTTTGGAAATAGGAGA197900.20144402487401097No Hit
CGTGTATTTGACAAGCTGAGTTG195550.19905194069789206No Hit
TGAGGGGCAGAGAGCGAGACTT193760.19722988509140155No Hit
TCCTGTACTGAGCTGCCCCGAGT187640.19100028715189196No Hit
AAGCCGGCGGGAGC180680.1839156463579399No Hit
TGAGGTAGTAGTTTGTACAGTT175360.17850037494647075No Hit
CGCGACCTCAGATC175000.17813392800885253No Hit
TGAGATGAAGCACTGTAGCTC172880.1759759627095453No Hit
AAAAGCTGGGTTGAGAGGGCG170840.17389943006304212No Hit
AGAAGCCGGCGGGAGC167000.16999066272844787No Hit
TAGCAGCACGTAAATATTGGCG166900.16988887191244278No Hit
TGAGAACTGAATTCCATGGGTT162450.1653591806002177No Hit
TGGCTCAGTTCAGCAGGAACA160740.1636185576465312No Hit
TTCAAGTAATTCAGGATAGGT157630.16045286326877387No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC150060.15274729849719093No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA149700.1523808515595727No Hit
AGCGCACGTGTTAGGAC141700.14423758627916805No Hit
TATTGCACTTGTCCCGGCCTG136090.13852712150128424No Hit
AAATGGATTTTTGGAGCAGGGAG135980.13841515160367868No Hit
GAAGAGGGGGACGG133070.13545303885793147No Hit
GGCTGGTCCGATGGTAGTGGGTTATC132920.1353003526339239No Hit
AAATGGATTTTTGGAGCAGGG126260.12852108428798698No Hit
TACAGTACTGTGATAACTGAAG123510.12572183684784788No Hit
TGAGGTAGTAGTTTGTGCTGT115990.11806716748426747No Hit
CGCGACCTCAGATCAGACG112030.11403625117046715No Hit
AGGCTGAGGCAGGAGA111730.11373087872245198No Hit
TAGCAGCACGTAAATATTGGC111570.11356801341684387No Hit
TGTAAACATCCCCGACTGGAAGC110080.1120513302583685No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA109650.11161362974954675No Hit
AAATGGATTTTTGGAGCAGGGAGAT109350.11130825730153159No Hit
GTTCTATTTTGTTGGTTTTCGGAAC109290.11124718281192855No Hit
AAATGGATTTTTGGAGCAGGGA109010.11096216852711438No Hit
TGTAAACATCCCCGACTGGAAGCT107380.10930297822623193No Hit
AGCTCGGTCTGAGGCCCCTCAGT107350.10927244098143041No Hit
CGCGACCTCAGATCAGAC106270.10817310016857577No Hit
TGTAAACATCCTTGACTGGAAGCT106130.10803059302616869No Hit
CAACGGAATCCCAAAAGCAGCTG105640.1075318180277439No Hit
AACATTCAACGCTGTCGGTGA105600.10749110170134187No Hit
TGAGATGAAGCACTGTAGCT103170.10501758487241894No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT99670.1014549063122419No Hit
GCGGGGTGGAGCAGTCTGGTAGCTCGTCGGG99610.10139383182263886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)