FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10379090
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT154012814.838757540400941No Hit
TCAGTGCACTACAGAACTTTGT2585342.4909120163713774No Hit
AAACCGTTACCATTACTGAGC2423422.3349060466765392No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1793301.7278007994920557No Hit
TATTGCACTTGTCCCGGCCTGT1606491.5478139220297733No Hit
TCCTGTACTGAGCTGCCCCGAG1587831.5298354672712156No Hit
TCCTGTACTGAGCTGCCCCGA1453321.4002383638642695No Hit
TGAGGTAGTAGGTTGTATAGT1213401.1690812971079352No Hit
TTCAAGTAATCCAGGATAGGCT1120161.0792468318513473No Hit
AAACCGTTACCATTACTGAG1063731.024877903554165No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC992780.9565193094963046No Hit
TGAGGTAGTAGGTTGTGTGGT973050.9375099358421596No Hit
TGAGGGGCAGAGAGCGAGACTTT887360.854949711390883No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT811140.7815136009033546No Hit
CGCGACCTCAGATCAGA805810.7763782759374859No Hit
TGAGGTAGTAGATTGTATAGT799220.7700289717113928No Hit
AAACCGTTACCATTACTGAGG762270.7344285481675176No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG751050.7236183518979024No Hit
TGAGGGGCAGAGAGCGAGACT740780.7137234574514721No Hit
AAGCTGCCAGTTGAAGAACTGT710850.6848866326431315No Hit
TGAGGTAGTAGGTTGTATAGTT701020.6754156674621764No Hit
TGAGGTAGTAGGTTGTGTGGTT676450.6517430718878052No Hit
TGGAGTGTGACAATGGTGTTT654240.6303442787373459No Hit
TGAGGTAGTAGTTTGTGCTGTT636480.6132329520218054No Hit
TCCTGTACTGAGCTGCCCCG562160.5416274451806469No Hit
TGAGGTAGTAGTTTGTACAGT561790.5412709592074064No Hit
CATTGCACTTGTCTCGGTCTGA541560.5217798477515851No Hit
TGAGGTAGTAGATTGTATAGTT536390.5167986788822526No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT498990.4807646913168688No Hit
CCCATAAAGTAGAAAGCACT489530.47165021210915403No Hit
TAGCTTATCAGACTGATGTTGAC460120.4433143946145568No Hit
TCAGTGCACTACAGAACTTTGC432650.4168477197904633No Hit
TAGCTTATCAGACTGATGTTGA419310.4039949552417409No Hit
TAGCTTATCAGACTGATGTTG375390.3616791067424986No Hit
TTCACAGTGGCTAAGTTCCG367920.35448194398545535No Hit
TGAGGTAGTAGGTTGTGTGG365280.35193836839260473No Hit
TGGCTCAGTTCAGCAGGAAC354580.3416291794367329No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT348010.33529914472270694No Hit
TCCTGTACTGAGCTGCCCCGAGA345040.33243762218074996No Hit
TCGTACCGTGAGTAATAATGCG343170.3306359228024807No Hit
AAACCGTTACCATTACTG337140.32482616491426514No Hit
TGAGGTAGTAGGTTGTATAG334580.3223596673696827No Hit
TATTGCACTTGTCCCGGCCTGC329630.31759046313308775No Hit
AAACCGTTACCATTACTGAGTT319300.30763776015045635No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC299220.28829117003513793No Hit
CGCGACCTCAGATC286190.2757370829234548No Hit
TCCTGTACTGAGCTGCCCCGAGT281060.27079445307825634No Hit
CGCGACCTCAGATCAGACGT269760.259907178760373No Hit
AAAAGCTGGGTTGAGAGGGCGA264990.25531140013238157No Hit
CATTGCACTTGTCTCGGTCTG252400.24318124228617347No Hit
TTCAAGTAATCCAGGATAGGCC221810.2137085235796202No Hit
TGAGGTAGTAGTTTGTGCTGT201820.1944486462686035No Hit
TATTGCACTTGTCCCGGCCTG197530.19031533593022124No Hit
TGTAAACATCCCCGACTGGAAG197180.18997811946904786No Hit
TTCAAGTAATTCAGGATAGGT191360.18437069145753626No Hit
TGAGGTAGTAGGTTGTATAGC190230.18328196402574792No Hit
TGAGGGGCAGAGAGCGAGACTT188430.18154770793971342No Hit
TGAGAACTGAATTCCATGGGTT186780.17995797319418178No Hit
AAGCTGCCAGTTGAAGAACT183300.17660507809451503No Hit
TGAGGTAGTAGGTTGTGTGGC178410.17189368239412126No Hit
TGAGGTAGTAGTTTGTACAGTT176350.16990892265121507No Hit
TGTAAACATCCCCGACTGGAAGCT174560.1681843013212141No Hit
TACAGTACTGTGATAACTGAAG173910.16755804217903497No Hit
TGAGGTAGTAGATTGTATAG173520.16718228669372748No Hit
AAACCGTTACCATTACTGAGA169450.16326094098808278No Hit
TAGCAGCACGTAAATATTGGCG165940.15987914162031547No Hit
TGTAAACATCCTTGACTGGAAGCT159730.15389595812349638No Hit
TGAGATGAAGCACTGTAGCT158490.15270124837533927No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC158310.15252782276673582No Hit
TGAGATGAAGCACTGTAGCTC158080.15230622337796473No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC145340.14003154419125377No Hit
CTAGACTGAAGCTCCTTGAGG143460.13822021005695106No Hit
AAAAGCTGGGTTGAGAGGGCG142530.13732417774583322No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG140840.13569590397616746No Hit
TCAGTGCACTACAGAACTTTGG136690.13169748022225455No Hit
TGGCTCAGTTCAGCAGGAACAG136170.13119647290851125No Hit
AGAAGCCGGCGGGAGC135480.130531674742198No Hit
TGAGGTAGTAGGTTGTAT135110.1301751887689576No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC126810.12217834126113176No Hit
TGAGGTAGTAGATTGTATAGC124470.1199238083492869No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCA123940.11941316627951004No Hit
TGAGGGGCAGAGAGCGAGACC123820.11929754920710775No Hit
AAGCTGCCAGTTGAAGAACTGC122430.11795831811844777No Hit
GCATTGGTGGTTCAGTGGTAGAATT121150.11672506934615656No Hit
TGAGGTAGTAGGTTGTGTGGTC117170.11289043644481356No Hit
TGAGGTAGTAGATTGTAT117040.11276518461637774No Hit
TACAGTATAGATGATGTACT114040.10987475780632021No Hit
TCCTGTACTGAGCTGCCCCGAA111920.1078321895272129No Hit
AGCTCGGTCTGAGGCCCCTCAGT111520.10744679928587188No Hit
GGCTGGTCCGATGGTAGTGGGT111070.10701323526436325No Hit
TGAGGTAGTAGGTTGTATAGTC108180.10422879077067451No Hit
AGACCGTTACCATTACTGAGT107450.10352545358022716No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT107190.10327494992335552No Hit
AGAGGTAGTAGGTTGCATAGT105570.10171411944592446No Hit
CGCGACCTCAGATCAGAC105460.10160813712955566No Hit
CAACGGAATCCCAAAAGCAGCT105340.10149252005715337No Hit
GGCTGGTCCGATGGTAGTGGGTT105260.10141544200888516No Hit
GAACCGTTACCATTACTGAGT105070.10123238164424818No Hit
CATTGCACTTGTCTCGGTCT104870.10103968652357768No Hit
TGAGGTAGTAGTTTGTGCTG104760.10093370420720892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)