FastQCFastQC Report
Thu 9 May 2024
EGAF00002167679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13278744
Total Bases314.5 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9290856.9967837319553725No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC7214095.432810512801512No Hit
TGGAGTGTGACAATGGTGTTT6183754.656878692743831No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3369872.537792730999257No Hit
TCCTGTACTGAGCTGCCCCGAG2507711.8885144558852855No Hit
TCCTGTACTGAGCTGCCCCGA2312661.7416255633815971No Hit
TTCAAGTAATCCAGGATAGGCT2214871.6679815500622648No Hit
TAGCTTATCAGACTGATGTTGAC2125031.6003245487675641No Hit
TGAGGTAGTAGGTTGTATAGTT1836161.3827813835404914No Hit
CGCGACCTCAGATCAGA1812901.3652646665979855No Hit
TCAGTGCACTACAGAACTTTGT1733521.3054849163444977No Hit
TGAGGTAGTAGGTTGTATAGT1649351.2420978972107604No Hit
TGAGGTAGTAGATTGTATAGTT1268250.9550978616652298No Hit
TATTGCACTTGTCCCGGCCTGT1233990.9292972287138No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1226310.9235135491730242No Hit
TCGTACCGTGAGTAATAATGCG1214010.9142506249085004No Hit
TGAGGTAGTAGGTTGTGTGGTT1124490.8468346102613319No Hit
TCCTGTACTGAGCTGCCCCGAGA1110600.8363742835918819No Hit
TGAGGGGCAGAGAGCGAGACTTT985260.7419828260865636No Hit
TGAGGTAGTAGATTGTATAGT957490.7210697035803989No Hit
TCCTGTACTGAGCTGCCCCGAGT921270.6937930274128337No Hit
AAGCTGCCAGTTGAAGAACTGT919040.6921136517128428No Hit
TGAGGTAGTAGTTTGTGCTGTT914040.6883482353451501No Hit
TGAGGTAGTAGGTTGTGTGGT904010.6807948101115587No Hit
AAACCGTTACCATTACTGAGC899610.6774812437079892No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC874420.6585110760475539No Hit
TAGCTTATCAGACTGATGTTGA660110.4971177996955134No Hit
TGAGGGGCAGAGAGCGAGACT623100.46924618774185267No Hit
TCCTGTACTGAGCTGCCCCG604690.4553819246760085No Hit
TGTAAACATCCCCGACTGGAAGCT518420.3904134306678403No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG507670.38231778547730116No Hit
TGAGGTAGTAGTTTGTACAGT498650.3755249743499837No Hit
CTAGACTGAAGCTCCTTGAGG494660.37252017208856497No Hit
TAGCTTATCAGACTGATGTTG488080.36756488414868155No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT487770.3673314283338846No Hit
AAAAGCTGGGTTGAGAGGGCGA478050.3600114589150901No Hit
AAATGGATTTTTGGAGCAGGG460650.3469078099555199No Hit
CATTGCACTTGTCTCGGTCTGA451330.33988907384614087No Hit
TGGAGTGTGACAATGGTGTTTG439620.3310704687130048No Hit
TGGCTCAGTTCAGCAGGAAC433780.3266724623955398No Hit
TGAGGTAGTAGGTTGTATAG387070.29149594268855544No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA380300.2863975689266997No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT368100.2772099529895297No Hit
TGAGGTAGTAGGTTGTGTGG357790.2694456644393476No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT354560.26701320546581814No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC337650.25427856731028176No Hit
CGCGACCTCAGATCAGACGT322010.24250034491213926No Hit
AAACCGTTACCATTACTGAG319480.24059504423008682No Hit
TGAGGTAGTAGTTTGTACAGTT317910.23941270349063132No Hit
TGTAAACATCCCCGACTGGAAGC311850.23484901885298792No Hit
TGGAGTGTGACAATGGTGTTC297470.22401968137950395No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA291340.21940328091271283No Hit
AACCCGTAGATCCGATCTTGT287400.21643613281497107No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC276320.20809197014416422No Hit
AAACCGTTACCATTACTG269810.20318939803342845No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC269280.20279026389845306No Hit
CCCATAAAGTAGAAAGCACT266780.20090755571460675No Hit
TTCAAGTAATCCAGGATAGGCC266200.20047076741595438No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT259530.19544770198145245No Hit
TGGCTCAGTTCAGCAGGAACAG259210.19520671533392014No Hit
TTCACAGTGGCTAAGTTCCG256990.19353487046666462No Hit
AAATGGATTTTTGGAGCAGGGA255100.19211154307967682No Hit
TATTGCACTTGTCCCGGCCTG227870.17160508554122286No Hit
CAACGGAATCCCAAAAGCAGCTG225670.16994830233943814No Hit
TGTAAACATCCCCGACTGGAAG218060.16421733862781No Hit
AGCTCGGTCTGAGGCCCCTCAGT213320.16064772391123738No Hit
CTAGACTGAAGCTCCTTGAGGA206210.1552933018363785No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA201840.15200232793101515No Hit
TATTGCACTTGTCCCGGCCTGTA198060.14915567315703954No Hit
TCGTACCGTGAGTAATAATGC193430.1456688976005562No Hit
TGAGAACTGAATTCCATGGGTT190870.14374100442029758No Hit
TTCAAGTAATTCAGGATAGGT190750.14365063442747295No Hit
CATTGCACTTGTCTCGGTCTG188710.14211434454945437No Hit
TGAGGGGCAGAGAGCGAGACTT186560.14049521551134656No Hit
TGAGGTAGTAGATTGTATAG181810.13691806996203856No Hit
TCAGTGCACTACAGAACTTTGC179630.13527634842572459No Hit
TACAGTACTGTGATAACTGAAG176700.13306981443425675No Hit
AAAAGCTGGGTTGAGAGGGCG174620.1315034012252966No Hit
AAATGGATTTTTGGAAATAGGA168960.12724094989706858No Hit
AAGCTGCCAGTTGAAGAACT167350.12602848582667156No Hit
AGAGGTAGTAGGTTGCATAGTT166740.12556910502981306No Hit
TGAGGTAGTAGGTTGTATAGC163870.12340775603475751No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA162110.1220823294733297No Hit
TAGCTTATCAGACTGATGTTGACT161050.12128406120337887No Hit
TGTAAACATCCTTGACTGGAAGCT158900.11966493216527105No Hit
TAGCTTATCAGACTGATGTTGACA154340.1162308724379354No Hit
AGCTACATCTGGCTACTGGGTCTCT154150.11608778661596308No Hit
AAATGGATTTTTGGAGCAGG149940.1129173060343659No Hit
CAACGGAATCCCAAAAGCAGCT149160.11232990108100585No Hit
TGAGGTAGTAGTTTGTGCTGT148520.1118479277859412No Hit
GGCTGGTCCGATGGTAGTGGGTTATC148140.11156175614199657No Hit
TAGCAGCACGTAAATATTGGC146290.11016855208595029No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG142860.10758547645771316No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT140890.10610190240884228No Hit
TGAGGTAGTAGGTTGTAT140290.10565005244471917No Hit
TGAGGTAGTAGGTTGTATAGTC139560.10510030165503605No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAC138290.10414388589764212No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC136670.10292389099450971No Hit
ACCAATATTACTGTGCTGCTT136530.10281845933621432No Hit
CGCGACCTCAGATCAGAC136270.10262265768509432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)