FastQCFastQC Report
Fri 10 May 2024
EGAF00002167680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524361
Total Bases311.5 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT5399723.992587893801415No Hit
TCAGTGCACTACAGAACTTTGT4840523.579111796853101No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2036561.5058456366256416No Hit
CGCGACCTCAGATCAGA1849351.3674213517370617No Hit
TATTGCACTTGTCCCGGCCTGT1632271.2069109956470403No Hit
TTCAAGTAATCCAGGATAGGCT1517681.1221824084701673No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1363211.0079662913464082No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1210240.8948592839247637No Hit
TGAGGTAGTAGGTTGTATAGTT1042900.7711270055568614No Hit
AAACCGTTACCATTACTGAGC894450.6613621153709222No Hit
AAGCTGCCAGTTGAAGAACTGT887900.6565190030050219No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT875110.6470619942783249No Hit
TCAGTGCACTACAGAACTTTGC863890.6387658537065078No Hit
TCCTGTACTGAGCTGCCCCGAG860000.6358895625456906No Hit
TCCTGTACTGAGCTGCCCCGA817560.6045091520405289No Hit
TGGAGTGTGACAATGGTGTTT799890.5914438397496192No Hit
TGAGGTAGTAGGTTGTATAGT765640.5661191682180031No Hit
TGAGGTAGTAGATTGTATAGTT749450.5541481774998464No Hit
TAGCTTATCAGACTGATGTTGAC706390.5223093349844773No Hit
TGAGGGGCAGAGAGCGAGACTTT700300.5178063495938922No Hit
TAGCTTATCAGACTGATGTTGA642500.47506865573907703No Hit
CATTGCACTTGTCTCGGTCTGA622310.46014003914861484No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT602070.4451744522347489No Hit
CGCGACCTCAGATC575700.4256763036715746No Hit
CCCATAAAGTAGAAAGCACT531300.39284665648898315No Hit
TGGCTCAGTTCAGCAGGAAC502510.37155914427306397No Hit
CGCGACCTCAGATCAGACGT486500.35972124671916106No Hit
TGAGGTAGTAGATTGTATAGT473420.3500498101167219No Hit
TTCACAGTGGCTAAGTTCCG472230.34916991641971107No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC462880.34225646594319686No Hit
TGAGGTAGTAGTTTGTGCTGTT411570.30431752006619756No Hit
TGAGGTAGTAGGTTGTGTGGTT382950.2831557069498515No Hit
TAGCTTATCAGACTGATGTTG361660.26741374324450523No Hit
TCGTACCGTGAGTAATAATGCG356100.26330264328200054No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC352900.2609365425841561No Hit
TATTGCACTTGTCCCGGCCTGC345640.25556845162592157No Hit
TGTAAACATCCCCGACTGGAAGCT337640.2496531998813105No Hit
TCCTGTACTGAGCTGCCCCGAGT332190.24562343463029418No Hit
TGAGGTAGTAGTTTGTACAGT323080.23888744170611834No Hit
TTCAAGTAATCCAGGATAGGCC311110.230036746283244No Hit
TGAGATGAAGCACTGTAGCT304300.22500138823564383No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT294140.21748901851998775No Hit
TCCTGTACTGAGCTGCCCCGAGA282480.20886753910221711No Hit
TGAGGGGCAGAGAGCGAGACT280880.2076844887532949No Hit
TGAAAAGAACTTTGAAGAGAG278900.20622046394650362No Hit
AAAAGCTGGGTTGAGAGGGCGA277920.2054958456077888No Hit
GCATTGGTGGTTCAGTGGTAGAATT273900.20252343160612174No Hit
TGAGGTAGTAGGTTGTGTGGT267240.197598984528733No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT265350.19620150630406866No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC252830.1869441373237523No Hit
TGGCTCAGTTCAGCAGGAACAG248100.183446744729751No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG247280.18284043142592835No Hit
TGTAAACATCCCCGACTGGAAG239950.17742058201492847No Hit
TGAGAACTGAATTCCATGGGTT237490.17560164210346058No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC235630.1742263460728385No Hit
TGTAAACATCCTTGACTGGAAGCT228110.16866600943290405No Hit
AAACCGTTACCATTACTGAG222210.16430351127125342No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT221320.16364543951466543No Hit
GGCTGGTCCGATGGTAGTGGGT219770.162499359489147No Hit
TGAGATGAAGCACTGTAGCTC219290.16214444438447037No Hit
TCCTGTACTGAGCTGCCCCG218790.16177474115043217No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT216910.16038465699044857No Hit
TGAGGTAGTAGTTTGTACAGTT211750.15656931961517442No Hit
GGCTGGTCCGATGGTAGT204180.15097201265183618No Hit
GTTGAAAAGAACTTTGAAGAGAG202910.15003296643737918No Hit
GGCTGGTCCGATGGTAGTGGGTT201650.14910131428760293No Hit
AGGAAACTCTGGTG197400.1459588367982783No Hit
TGAGGTAGTAGGTTGTATAG195650.14466487547914464No Hit
CTAGACTGAAGCTCCTTGAGG194480.14379976991149526No Hit
TGAGGGGCAGAGAGCGAGACTT193650.14318606254299188No Hit
AGCTCGGTCTGAGGCCCCTCAGT184150.1361617010962662No Hit
CATTGCACTTGTCTCGGTCTG184020.1360655782554163No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT181960.13454240093117892No Hit
TGAGGTAGTAGGTTGTATAGTC181960.13454240093117892No Hit
TGTAAACATCCCCGACTGGAAGC167160.12359918520364845No Hit
TCAGTGCATCACAGAACTTTGT166840.12336257513386399No Hit
AAGCTGCCAGTTGAAGAACTGC163370.12079683468963895No Hit
GGCTGGTCCGATGGTAGTG159870.11820891205137159No Hit
TGAGGTAGTAGTTTGTGCTGT159050.11760259874754897No Hit
AAGTTGAAAAGAACTTTGAAGAGAG155250.11479285416885869No Hit
CGCGACCTCAGATCAGAC154960.11457842629311656No Hit
CGTGTATTTGACAAGCTGAGTTG154120.11395732485993239No Hit
TTCAAGTAATTCAGGATAGGT153950.11383162576035939No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT151320.11188698674931852No Hit
TAGCTTATCAGACTGATGTTGAT145770.10778328085149456No Hit
CAACGGAATCCCAAAAGCAGCTG144650.10695514560724903No Hit
TGAGGTAGTAGGTTGTGTGG144340.10672592960214536No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC141200.1044041932923855No Hit
TGGAGAGAAAGGCAGTTCCTGA137730.10183845284816044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)