FastQCFastQC Report
Wed 8 May 2024
EGAF00002167681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15416994
Total Bases393.5 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT5312583.445924672475062No Hit
CGCGACCTCAGATCAGA2170171.4076479500478498No Hit
TCAGTGCACTACAGAACTTTGT1565471.0154184401965778No Hit
TGAGGTAGTAGGTTGTATAGT1268500.8227933409067941No Hit
TGAGGTAGTAGGTTGTATAGTT1256710.8151459357122406No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1223830.7938188209711958No Hit
TTCAAGTAATCCAGGATAGGCT1069240.6935463554049512No Hit
TCCTGTACTGAGCTGCCCCGAG1055060.6843487128554373No Hit
TCCTGTACTGAGCTGCCCCGA949030.6155739568945802No Hit
TGGCTCAGTTCAGCAGGAAC851280.5521698977115772No Hit
TGAGGTAGTAGATTGTATAGTT823110.5338978532390946No Hit
TGAGGTAGTAGATTGTATAGT715820.4643058173337811No Hit
TTCACAGTGGCTAAGTTCCG696500.45177419151878767No Hit
AAGCTGCCAGTTGAAGAACTGT639600.4148668670429527No Hit
TAGCTTATCAGACTGATGTTGAC621100.4028671218267323No Hit
TCGTACCGTGAGTAATAATGCG614920.39885855828963807No Hit
AAAAGCTGGGTTGAGAGGGCGA549600.35648972815323143No Hit
TGAGGGGCAGAGAGCGAGACTTT522840.33913225885668763No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT504230.3270611638040464No Hit
CGCGACCTCAGATCAGACGT500170.32442770620524336No Hit
TAGCTTATCAGACTGATGTTG479730.3111696093285111No Hit
TATTGCACTTGTCCCGGCCTGT462630.3000779529394641No Hit
AAACCGTTACCATTACTGAGC453520.29416888921407114No Hit
TGAGGTAGTAGGTTGTGTGGTT447490.29025762090845986No Hit
TAGCTTATCAGACTGATGTTGA399740.25928530555307994No Hit
TGAGGTAGTAGGTTGTATAG382120.24785635902822561No Hit
TGAGGTAGTAGGTTGTGTGGT364650.23652470773485418No Hit
TCCTGTACTGAGCTGCCCCGAGA360160.2336123371391336No Hit
AAACCGTTACCATTACTGAG353180.22908486570079745No Hit
TGAGGGGCAGAGAGCGAGACT326800.21197387765734357No Hit
TGAGGTAGTAGTTTGTGCTGTT322730.20933393370977507No Hit
TCCTGTACTGAGCTGCCCCGAGT293110.19012136866629123No Hit
CCCATAAAGTAGAAAGCACT290780.188610049403924No Hit
TGAGGTAGTAGTTTGTACAGT285030.18488039886374738No Hit
TCCTGTACTGAGCTGCCCCG272550.17678543560437268No Hit
TGGAGAGAAAGGCAGTTCCTGA259740.16847642283573566No Hit
AAATGGATTTTTGGAGCAGGGAGA254770.16525270749926996No Hit
TGAGGTAGTAGGTTGTGTGG240580.15604857860099058No Hit
TGGAGTGTGACAATGGTGTTT236360.1533113394219392No Hit
CATTGCACTTGTCTCGGTCTGA236140.15316863974909764No Hit
AAACCGTTACCATTACTG219830.14258940491252706No Hit
GGCTGGTCCGATGGTAGTG215790.1399689200112551No Hit
TGGCTCAGTTCAGCAGGAACAG214330.13902191309148854No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG199290.12926644454813954No Hit
TTCAAGTAATTCAGGATAGGT197590.12816376525800036No Hit
TGAGGTAGTAGTTTGTACAGTT187360.12152823047086872No Hit
TGAGGTAGTAGATTGTATAG186490.12096391812826807No Hit
AAAAGCTGGGTTGAGAGGGCG184880.1199196159770186No Hit
TCCTTTACTGAGCTGCCCCGAG184210.11948503060972845No Hit
CGCTACCTCAGATCAGA183000.11870018240909999No Hit
ATTTTTGGAGCAGGGAGA180540.11710454061278094No Hit
TGAGTTAGTAGGTTGTATAGT180500.11707859521771884No Hit
GGCTTGTCCGATGGTAGTGGGTTATCAGAAC179990.11674779143067708No Hit
TGAGGGGCAGAGAGCGAGACTT179600.11649482382882162No Hit
TGTAAACATCCCCGACTGGAAG179210.11624185622696617No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT178670.11589159339362783No Hit
TGAGTTAGTAGGTTGTATAGTT177500.11513269058806147No Hit
AAACGGTTACCATTACTGAGT175340.11373163925470814No Hit
CGCGACCTCAGATCAGAC172650.11198681143678203No Hit
ACGGCCCTGGCGGA167980.10895768656328204No Hit
CGCGACCTCAGATC164750.1068625959120176No Hit
TCAGTGCACTACAGAACTTTGC164410.10664206005398978No Hit
TTTTTGGAGCAGGGAGA162980.10571451218051976No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA161750.10491669128236024No Hit
ACGGCCCTGGCGGAGC158290.10267241460948871No Hit
TCATTGCACTACAGAACTTTGT157300.10203026608170179No Hit
TCCTTTACTGAGCTGCCCCGA154880.10046056968044484No Hit
GGCTGGTCCGATGGTAGTGGGT154220.10003247066192021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)