FastQCFastQC Report
Wed 8 May 2024
EGAF00002167682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18288941
Total Bases454.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8086224.42137136316422No Hit
TCAGTGCACTACAGAACTTTGT4630392.5317977678423262No Hit
CGCGACCTCAGATCAGA2633761.440083381536416No Hit
TCCTGTACTGAGCTGCCCCGAG1559870.8529034021160656No Hit
TCCTGTACTGAGCTGCCCCGA1209300.6612192581298174No Hit
TTCAAGTAATCCAGGATAGGCT1026800.5614321791513244No Hit
TATTGCACTTGTCCCGGCCTGT994550.5437985720441659No Hit
TTCACAGTGGCTAAGTTCCG948640.5186959704227817No Hit
CATTGCACTTGTCTCGGTCTGA917520.5016802230375176No Hit
TGAGGTAGTAGGTTGTATAGTT915120.500367954601636No Hit
TGAGGTAGTAGGTTGTATAGT915080.500346083461038No Hit
AAACCGTTACCATTACTGAGC868830.4750575771445706No Hit
TAGCTTATCAGACTGATGTTGA730120.3992139293357663No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC689420.376960043777275No Hit
AAGCTGCCAGTTGAAGAACTGT665110.3636678580788248No Hit
TGAGGTAGTAGGTTGTGTGGTT643870.352054282421273No Hit
TGGCTCAGTTCAGCAGGAAC638290.34900325830784845No Hit
TGAGGTAGTAGATTGTATAGTT618130.33798020344644336No Hit
CCCATAAAGTAGAAAGCACT603980.33024328745989173No Hit
TAGCTTATCAGACTGATGTTGAC580090.3171807487377208No Hit
CGCGACCTCAGATC574820.3142992259639309No Hit
TCAGTGCACTACAGAACTTTGC566760.3098921911334287No Hit
TCCTGTACTGAGCTGCCCCGAGA551680.30164677112797295No Hit
TGAGGTAGTAGATTGTATAGT550880.30120934831601237No Hit
CGCGACCTCAGATCAGACGT549250.30031809933664283No Hit
TGAGGTAGTAGGTTGTGTGGT528960.2892239632682942No Hit
TGAGGGGCAGAGAGCGAGACTTT500890.2738758903536296No Hit
TAGCTTATCAGACTGATGTTG490610.2682550072199369No Hit
TGGAGTGTGACAATGGTGTTT490220.26804176359910614No Hit
TCGTACCGTGAGTAATAATGCG473390.25883948119248673No Hit
TCATTGCACTACAGAACTTTGT463980.25369429536680116No Hit
AAACCGTTACCATTACTGAG460160.2516056014396897No Hit
TGAGGTAGTAGGTTGTATAG447740.24481461228400267No Hit
TCCTGTACTGAGCTGCCCCGAGT407050.22256619451066084No Hit
TGAGGTAGTAGTTTGTACAGT391090.21383960941204852No Hit
TGAGGTAGTAGTTTGTGCTGTT390290.21340218660008797No Hit
TGAGATGAAGCACTGTAGCT380340.20796174037632909No Hit
TCCTGTACTGAGCTGCCCCG368440.20145507604841634No Hit
TCAGGGCACTACAGAACTTTGT354240.19369082113611719No Hit
TGGCTCAGTTCAGCAGGAACAG339240.18548914341185746No Hit
TGAGGGGCAGAGAGCGAGACT329510.18016898846138768No Hit
CATTGCACTTGTCTCGGTCTG327690.17917385156417748No Hit
TGTAAACATCCCCGACTGGAAG326170.17834274822145252No Hit
AAAAGCTGGGTTGAGAGGGCGA325140.17777956635105335No Hit
AAACCGTTACCATTACTG296890.1623330733036976No Hit
TGAGGTAGTAGGTTGTGTGG290150.1586477861129302No Hit
CGTGTATTTGACAAGCTGAGTTG279650.1529066117059484No Hit
TCCTTTACTGAGCTGCCCCGAG269600.14741148763069442No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT253410.13855914347364343No Hit
TAACCGTTACCATTACTGAGT252870.1382638830755701No Hit
TTCAAGTAATTCAGGATAGGT246980.1350433576225108No Hit
TGAGATGAAGCACTGTAGCTC246140.13458406366995224No Hit
AAACGGTTACCATTACTGAGT238590.13045588588207485No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC232670.12721895707356703No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT222510.12166368736166845No Hit
CGCTACCTCAGATCAGA220010.12029674107429184No Hit
TGAAAAGAACTTTGAAGAGAG218180.11929613639193215No Hit
TGAGGTAGTAGTTTGTACAGTT215260.11769954312827627No Hit
TGGCTCAGTTCAGCAGGAACA213950.11698326327369091No Hit
TGGAGAGAAAGGCAGTTCCTGA213640.11681376193405622No Hit
TCCTTTACTGAGCTGCCCCGA212030.11593344852498567No Hit
TGAGGTAGTAGATTGTATAG207800.11362057540674443No Hit
CTAGACTGAAGCTCCTTGAGG196980.10770443187497844No Hit
ATTTTTGGAGCAGGGAGA184580.1009243782895904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)