FastQCFastQC Report
Thu 9 May 2024
EGAF00002167684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12242034
Total Bases280.1 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT124822310.196205957278014No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4341733.546575675251351No Hit
TCCTGTACTGAGCTGCCCCGAG3381872.7625066226739774No Hit
TCCTGTACTGAGCTGCCCCGA3061222.5005811942688605No Hit
TGGAGTGTGACAATGGTGTTT3056102.496398882734683No Hit
CGCGACCTCAGATCAGA2115771.7282830614585778No Hit
TATTGCACTTGTCCCGGCCTGT2095811.7119785813370556No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG1804981.4744118501876404No Hit
TCAGTGCACTACAGAACTTTGT1727181.4108603194534501No Hit
AAACCGTTACCATTACTGAGC1421151.160877350936944No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1381321.1283419078888361No Hit
TTCAAGTAATCCAGGATAGGCT1140980.9320183230989229No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1129630.9227469879596806No Hit
AAGCTGCCAGTTGAAGAACTGT994000.8119565751900379No Hit
TGAGGGGCAGAGAGCGAGACTTT992980.8111233803140883No Hit
TCCTGTACTGAGCTGCCCCG895090.7311611779545785No Hit
TAGCTTATCAGACTGATGTTGAC851250.6953501354431788No Hit
TCCTGTACTGAGCTGCCCCGAGA846000.6910616324052032No Hit
AAACCGTTACCATTACTGAG813080.6641706762127928No Hit
CATTGCACTTGTCTCGGTCTGA791150.6462569863798777No Hit
TAGCTTATCAGACTGATGTTGA767300.626774929721646No Hit
TGAGGGGCAGAGAGCGAGACT736060.6012562945013876No Hit
AAAAGCTGGGTTGAGAGGGCGA711440.581145257397586No Hit
TGAGGTAGTAGGTTGTATAGTT701440.5729766801823946No Hit
TAGCTTATCAGACTGATGTTG576400.47083679068363965No Hit
TGAGGTAGTAGGTTGTATAGT558910.4565499491342697No Hit
TCGTACCGTGAGTAATAATGCG543990.44436243192920394No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC541860.44262252498236815No Hit
TGAGGTAGTAGATTGTATAGTT526480.43005925322540356No Hit
TCCTGTACTGAGCTGCCCCGAGT503890.4116064372962859No Hit
TGAGGTAGTAGGTTGTGTGGTT496160.40529212710894286No Hit
TGAGGTAGTAGTTTGTGCTGTT490910.40100362407096724No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC436430.3565012154026039No Hit
TGGAGTGTGACAATGGTGTTTG424610.3468459571342475No Hit
TGAGGTAGTAGGTTGTGTGGT421020.3439134379139937No Hit
TATTGCACTTGTCCCGGCCTG417550.34107894162032226No Hit
AAACCGTTACCATTACTG415760.339616766298803No Hit
TGAGGTAGTAGGTTGTGTGG397360.3245865842228505No Hit
TGTAAACATCCCCGACTGGAAG397240.32448856129626824No Hit
CATTGCACTTGTCTCGGTCTG389250.3179618681013302No Hit
CTAGACTGAAGCTCCTTGAGG388590.3174227420051276No Hit
CGCGACCTCAGATC388170.31707966176208957No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG364870.29804687685069325No Hit
TGAGGTAGTAGATTGTATAGT361760.29550644933676873No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC349050.2851241876962603No Hit
TGTAAACATCCCCGACTGGAAGCT337340.275558783777271No Hit
TGGCTCAGTTCAGCAGGAAC335760.2742681485772708No Hit
TGAGGTAGTAGGTTGTATAG331330.2706494688709409No Hit
TACAGTACTGTGATAACTGAAG310290.25346278241017794No Hit
CCCATAAAGTAGAAAGCACT298360.24371766979245443No Hit
TTCACAGTGGCTAAGTTCCG279250.2281075187342234No Hit
TGAGGTAGTAGTTTGTACAGT277760.22689040072915986No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT271110.22145829688105753No Hit
TGTAAACATCCCCGACTGGAAGC266080.21734950254181618No Hit
TGAGGGGCAGAGAGCGAGACTT253580.2071387810228268No Hit
TATTGCACTTGTCCCGGCCTGC253470.20704892667345967No Hit
TGAGGTAGTAGTTTGTGCTGT251570.2054968970025733No Hit
AAAAGCTGGGTTGAGAGGGCG235530.19239449914940607No Hit
CGCGACCTCAGATCAGACGT233180.19047488350383604No Hit
TGAGAACTGAATTCCATGGGTT228900.18697873245573407No Hit
TCAGTGCACTACAGAACTTTGC212080.1732391855797819No Hit
TGGCTCAGTTCAGCAGGAACAG208970.17069875806585735No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA202800.1656587459240842No Hit
TGTAAACATCCTTGACTGGAAGCT191090.15609334200509492No Hit
TGGAGTGTGACAATGGTGTTC183650.15001592055699242No Hit
CGCGACCTCAGATCAGAC180960.1478185732861059No Hit
AAGCTGCCAGTTGAAGAACT169510.1384655523747116No Hit
TAGCAGCACGTAAATATTGGCG167500.13682366835445808No Hit
CAACGGAATCCCAAAAGCAGCTG166670.1361456764455972No Hit
TGAGGTAGTAGATTGTATAG162340.13260868251141927No Hit
AAAAGCTGGGTTGAGAGGGCGAA159490.13028063800508966No Hit
TTCAAGTAATCCAGGATAGGCC158980.1298640405671149No Hit
AACCCGTAGATCCGATCTTGT153890.12570623476458243No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCA152500.1245708025316708No Hit
ACCAATATTACTGTGCTGCTT149240.12190784635951837No Hit
TACAGTACTGTGATAACTGA148610.12139322599496129No Hit
TGAGGTAGTAGTTTGTGCTG144900.11836268384812525No Hit
GGCTGGTCCGATGGTAGTGGGTTATC143900.11754582612660608No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA142400.11632053954432736No Hit
AAAAGCTGGGTTGAGAGGGCGT141930.11593661641521336No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT141120.11527496166078284No Hit
TGAGGTAGTAGTTTGTACAGTT139600.11403333792407372No Hit
CTAGACTGAAGCTCCTTGAGGA139250.11374743772154203No Hit
AAACCGTTACCATTACTGAGTT139150.11366575194939012No Hit
TCCTGTACTGAGCTGCCCCGAA136470.11147657325571879No Hit
AGCTCGGTCTGAGGCCCCTCAGT135520.1107005584202756No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC135490.11067605268863No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC135210.11044733252660464No Hit
TGTAAACATCCCCGACTGGAA134760.11007974655192103No Hit
TGGAGAGAAAGGCAGTTCCTG127850.10443525969622369No Hit
AAGCTGCCAGTTGAAGAACTGC124870.10200102368609662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)