FastQCFastQC Report
Wed 8 May 2024
EGAF00002167685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9942341
Total Bases227.5 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7299987.342315054372003No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3388283.407929782331948No Hit
CGCGACCTCAGATCAGA2238032.2510090933312386No Hit
TCCTGTACTGAGCTGCCCCGAG2141032.1534465575059234No Hit
TCCTGTACTGAGCTGCCCCGA1697171.707012463161342No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1657851.667464433175245No Hit
TTCAAGTAATCCAGGATAGGCT1385051.3930823736582763No Hit
TATTGCACTTGTCCCGGCCTGT1363431.3713369919619534No Hit
TCAGTGCACTACAGAACTTTGT1130521.1370762680539725No Hit
TGGAGTGTGACAATGGTGTTT1120651.127149028583912No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1084481.0907692665137918No Hit
TAGCTTATCAGACTGATGTTGAC1037271.0432854797476772No Hit
TGAGGTAGTAGGTTGTATAGTT947730.9532262069868656No Hit
TGAGGTAGTAGGTTGTATAGT826220.8310115293772362No Hit
AAACCGTTACCATTACTGAGC788890.7934650400745659No Hit
TGAGGTAGTAGATTGTATAGTT736100.7403688930001495No Hit
AAGCTGCCAGTTGAAGAACTGT734380.7386389181380925No Hit
TCCTGTACTGAGCTGCCCCGAGA698340.702389909981965No Hit
AAACCGTTACCATTACTGAG692740.6967574336868952No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC669870.6737548028175658No Hit
TGAGGGGCAGAGAGCGAGACTTT667110.6709787966435671No Hit
TAGCTTATCAGACTGATGTTGA627160.630797113074275No Hit
TCGTACCGTGAGTAATAATGCG594450.5978974167150373No Hit
TGAGGTAGTAGTTTGTGCTGTT579910.583273094334624No Hit
CGCGACCTCAGATC554750.5579671829803463No Hit
TGAGGTAGTAGATTGTATAGT540880.5440167461566647No Hit
TCCTGTACTGAGCTGCCCCG533500.5365939470392336No Hit
CATTGCACTTGTCTCGGTCTGA507000.5099402645714928No Hit
TGAGGTAGTAGGTTGTGTGGTT477920.4806916198106663No Hit
AAAAGCTGGGTTGAGAGGGCGA451260.45387700944878073No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG450300.4529114420839116No Hit
TAGCTTATCAGACTGATGTTG428550.43103530647359606No Hit
TCCTGTACTGAGCTGCCCCGAGT421900.42434674087320085No Hit
TGAGGTAGTAGGTTGTGTGGT411140.4135243399919597No Hit
TGAGGGGCAGAGAGCGAGACT376200.37838171110807806No Hit
TGAGGTAGTAGTTTGTACAGT333440.33537373139786697No Hit
TGAGGTAGTAGGTTGTATAG319440.3212925406601926No Hit
TGTAAACATCCCCGACTGGAAGCT319340.3211919607263521No Hit
AAACCGTTACCATTACTG287030.28869458410247645No Hit
TGGCTCAGTTCAGCAGGAAC274360.2759511064848812No Hit
CTAGACTGAAGCTCCTTGAGG268090.2696447446330799No Hit
TATTGCACTTGTCCCGGCCTG262850.26437435609983606No Hit
CGCGACCTCAGATCAGACGT260800.26231246745610515No Hit
TGTAAACATCCCCGACTGGAAG260410.2619202057141271No Hit
CATTGCACTTGTCTCGGTCTG243710.2451233567627584No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT238190.23957134441476108No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC227080.2283969137650781No Hit
CCCATAAAGTAGAAAGCACT219380.22065225885935716No Hit
TGTAAACATCCCCGACTGGAAGC219290.2205617369189007No Hit
CAACGGAATCCCAAAAGCAGCTG214960.2162066257836057No Hit
CGCGACCTCAGATCAGAC210610.21183139866154257No Hit
TACAGTACTGTGATAACTGAAG206500.20769756338069675No Hit
TGAGGTAGTAGTTTGTACAGTT204210.2053942828957486No Hit
TTCACAGTGGCTAAGTTCCG200690.20185386922456192No Hit
TGAGGTAGTAGGTTGTGTGG200140.201300679588439No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA199490.20064691001847554No Hit
TGAGAACTGAATTCCATGGGTT189410.19050845268735No Hit
TTCAAGTAATCCAGGATAGGCC184580.18565044188285235No Hit
TGAGGTAGTAGATTGTATAG179790.1808326630518909No Hit
TGAGGGGCAGAGAGCGAGACTT170780.17177041101285906No Hit
TGGCTCAGTTCAGCAGGAACAG170420.1714083232510331No Hit
TGAAAAGAACTTTGAAGAGAG166380.16734489392387566No Hit
TGAGGTAGTAGTTTGTGCTGT157060.15797084408993817No Hit
TGTAAACATCCTTGACTGGAAGCT156040.15694492876476476No Hit
TATTGCACTTGTCCCGGCCTGC155110.1560095353800478No Hit
AGCTCGGTCTGAGGCCCCTCAGT145130.14597165798276282No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC139420.14022854376046848No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA139010.1398161660317223No Hit
AAAAGCTGGGTTGAGAGGGCG137910.13870978675947646No Hit
GGCTGGTCCGATGGTAGTGGGTTATC137200.1379956692292087No Hit
TGGAGTGTGACAATGGTGTTTG136310.13710050781802796No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT135380.13616511443331103No Hit
AAACCGTTACCATTACTGAGTT128520.1292653309718506No Hit
TCAGTGCACTACAGAACTTTGC127750.12849086548127853No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC127570.12830982160036553No Hit
TTCAAGTAATTCAGGATAGGT127120.12785721189808316No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA124040.1247593499357948No Hit
CGCGACCTCAGATCAGACG120110.12080655853586193No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC118130.1188150758458194No Hit
CAACGGAATCCCAAAAGCAGCT116860.11753771068604467No Hit
TACAGTATAGATGATGTACT115680.1163508674667264No Hit
GGCTGGTCCGATGGTAGTG112590.11324294751105397No Hit
TATTGCACTTGTCCCGGCCTGTA107350.10797255897781015No Hit
CTAGACTGAAGCTCCTTGAGGA106950.10757023924244803No Hit
GCATTGGTGGTTCAGTGGTAGAATT106230.10684606371879621No Hit
GGCTGGTCCGATGGTAGTGGGTT105530.1061420041819125No Hit
AGAGGTAGTAGGTTGCATAGTT103050.10364762182266732No Hit
TGGAGAGAAAGGCAGTTCCTG102980.10357721586897894No Hit
AAGCTGCCAGTTGAAGAACT102080.10267199646441416No Hit
TAGCAGCACGTAAATATTGGCG101530.10211880682829125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)