FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12041420
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT126502410.505604820693906No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6320355.248841083526694No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3241352.6918336873890287No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2250461.868932401660269No Hit
TCCTGTACTGAGCTGCCCCGAG1967831.6342175590586494No Hit
TGGAGTGTGACAATGGTGTTT1806841.5005207027078202No Hit
TCAGTGCACTACAGAACTTTGT1691311.4045768688410503No Hit
TTCAAGTAATCCAGGATAGGCT1507171.2516547051759677No Hit
TCCTGTACTGAGCTGCCCCGA1505181.2500020761670967No Hit
CGCGACCTCAGATCAGA1292511.0733866935959382No Hit
TAGCTTATCAGACTGATGTTGAC1286181.0681298385074185No Hit
AAACCGTTACCATTACTGAGC1283291.0657297893437816No Hit
TGAGGTAGTAGGTTGTATAGT1158980.9624944566338521No Hit
TATTGCACTTGTCCCGGCCTGT1154940.9591393706057925No Hit
TGAGGTAGTAGGTTGTGTGGT995710.8269041358909497No Hit
TCGTACCGTGAGTAATAATGCG913210.7583906217040848No Hit
TGAGGTAGTAGGTTGTGTGGTT889840.7389826116853327No Hit
AAGCTGCCAGTTGAAGAACTGT886160.7359264937191793No Hit
TGAGGTAGTAGGTTGTATAGTT874430.7261851177020651No Hit
TAGCTTATCAGACTGATGTTGA871400.7236688031810202No Hit
TGAGGTAGTAGATTGTATAGT840690.6981651665667338No Hit
TGAGGTAGTAGATTGTATAGTT793930.6593325371924573No Hit
TGAGGTAGTAGTTTGTGCTGTT752480.6249096867312991No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC738330.6131585809647035No Hit
AAACCGTTACCATTACTGAG735450.610766836469453No Hit
TGAGGGGCAGAGAGCGAGACTTT700830.5820160745161285No Hit
TAGCTTATCAGACTGATGTTG688790.5720172537790393No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC622260.5167662950050741No Hit
TCCTGTACTGAGCTGCCCCGAGA614990.5107288010882437No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG560490.4654683583829814No Hit
TGAGGTAGTAGTTTGTACAGT554920.4608426580918198No Hit
TGGCTCAGTTCAGCAGGAAC554520.46051047135636825No Hit
TGAGGGGCAGAGAGCGAGACT527310.43791346867728226No Hit
CATTGCACTTGTCTCGGTCTGA518450.43055553248703227No Hit
TGAGGTAGTAGGTTGTGTGG507480.4214453112672758No Hit
AAACCGTTACCATTACTG500050.4152749426562648No Hit
TCCTGTACTGAGCTGCCCCG493490.40982708019486075No Hit
TGAGGTAGTAGGTTGTATAG478000.3969631488645027No Hit
AAAAGCTGGGTTGAGAGGGCGA431190.3580889961482948No Hit
CTAGACTGAAGCTCCTTGAGG413120.3430824603742748No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT405250.3365466863542672No Hit
CGCGACCTCAGATCAGACGT399560.33182133004247005No Hit
CCCATAAAGTAGAAAGCACT384270.31912349207983776No Hit
TTCACAGTGGCTAAGTTCCG381110.31649921686977117No Hit
TGGCTCAGTTCAGCAGGAACAG377390.31340988023007255No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC356500.2960614279711197No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT326030.2707571033981042No Hit
TGAGGTAGTAGATTGTATAG306680.25468757007063947No Hit
TCCTGTACTGAGCTGCCCCGAGT301690.25054354054588246No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT290050.24087690654424476No Hit
TGAGAACTGAATTCCATGGGTT284580.236334252936946No Hit
TGTAAACATCCCCGACTGGAAGCT264510.21966678348566862No Hit
TACAGTACTGTGATAACTGAAG258780.21490820850032635No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA253870.21083061632265962No Hit
CATTGCACTTGTCTCGGTCTG252560.20974270476405604No Hit
TGTAAACATCCCCGACTGGAAG251840.20914476864024342No Hit
CGCGACCTCAGATC249790.20744231162105464No Hit
TTCAAGTAATTCAGGATAGGT246940.20507548113096294No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC243970.20260899462023582No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT237840.1975182328994421No Hit
TGAGGTAGTAGTTTGTGCTGT236160.1961230486105459No Hit
TGAGGTAGTAGTTTGTACAGTT218950.18183071431774658No Hit
TATTGCACTTGTCCCGGCCTG212790.176715038591794No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT203380.16890034564029824No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC203060.16863459625193705No Hit
TGTAAACATCCCCGACTGGAAGC201740.16753838002494723No Hit
TTCAAGTAATCCAGGATAGGCC189980.1577720900026741No Hit
TACAGTATAGATGATGTACT188150.15625233568798363No Hit
TCAGTGCACTACAGAACTTTGC187110.15538865017580983No Hit
AAAAGCTGGGTTGAGAGGGCG175170.14547287612258356No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT174420.14485002599361205No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC165190.13718481707306945No Hit
TGAGGGGCAGAGAGCGAGACTT159430.13240132808256835No Hit
AAGCTGCCAGTTGAAGAACT158350.13150442389684935No Hit
TGGAGAGAAAGGCAGTTCCTG158330.1314878145600768No Hit
CTAGACTGAAGCTCCTTGAGGA157530.13082344108917388No Hit
TGTAAACATCCTTGACTGGAAGCT153110.12715277766243516No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA150430.12492712653491032No Hit
TCGTACCGTGAGTAATAATGC148310.12316653683701756No Hit
AGAAGCCGGCGGGAGC145460.12079970634692587No Hit
AACCCGTAGATCCGATCTTGT145070.12047582427986067No Hit
TGAGGTAGTAGGTTGTAT143060.11880658593421706No Hit
TAGCAGCACGTAAATATTGGC139950.11622383406608192No Hit
TGGAGAGAAAGGCAGTTCCTGA139860.11614909205060533No Hit
CAACGGAATCCCAAAAGCAGCTG138530.1150445711552292No Hit
TGAGGTAGTAGATTGTAT138050.11464594707268744No Hit
AGCTCGGTCTGAGGCCCCTCAGT137840.11447154903657542No Hit
TGGCTCAGTTCAGCAGGAACA136390.11326737212056384No Hit
TGAGGTAGTAGTTTGTGCTG136320.11320923944185984No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC136080.11300992740058896No Hit
AGTTGGTCCGAGTGTTGT136000.11294349005349867No Hit
ACCAATATTACTGTGCTGCTT133690.11102511165626645No Hit
TGAGATGAAGCACTGTAGCTC132670.11017803548086522No Hit
TGGAGTGTGACAATGGTGTTTG131540.10923960795321483No Hit
AAACCGTTACCATTACTGAGTT129100.10721326886696086No Hit
TACAGTACTGTGATAACTGA127260.10568520988388412No Hit
TCAGTGCATCACAGAACTTTGT126600.10513710177038921No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT126540.10508727376007149No Hit
TATTGCACTTGTCCCGGCCTGC123570.10262078724934436No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG123520.10257926390741291No Hit
TGAGGTAGTAGGTTGTATAGC123210.10232181918743803No Hit
TAGCAGCACGTAAATATTGGCG123120.10224707717196144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)