FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12441467
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT129438210.403773124182221No Hit
AAACCGTTACCATTACTGAGC2381811.9144125045704015No Hit
TTCAAGTAATCCAGGATAGGCT1822091.4645298661323458No Hit
TGAGGTAGTAGGTTGTATAGT1760621.415122509266793No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1623921.3052480065252754No Hit
TCAGTGCACTACAGAACTTTGT1503261.2082658741127552No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1359881.0930222296132763No Hit
TATTGCACTTGTCCCGGCCTGT1348891.084188866152199No Hit
TGAGGTAGTAGATTGTATAGT1310891.0536458441757712No Hit
TGAGGTAGTAGGTTGTATAGTT1112040.89381742522807No Hit
TGAGGTAGTAGTTTGTGCTGTT1084300.8715210191852778No Hit
CGCGACCTCAGATCAGA1062330.8538623299004853No Hit
TGAGGTAGTAGATTGTATAGTT1019900.8197586345725949No Hit
TGAGGTAGTAGGTTGTGTGGT985970.79248693100259No Hit
TCCTGTACTGAGCTGCCCCGAG957710.7697725678169625No Hit
TCCTGTACTGAGCTGCCCCGA872180.7010266554579134No Hit
TGAGGTAGTAGGTTGTGTGGTT848840.6822668098544971No Hit
AAGCTGCCAGTTGAAGAACTGT844570.67883473870083No Hit
TGGAGTGTGACAATGGTGTTT776390.624034127165229No Hit
TGAGGTAGTAGTTTGTACAGT775260.6231258741432983No Hit
TAGCTTATCAGACTGATGTTGAC707540.5686949939263594No Hit
TGAGGGGCAGAGAGCGAGACTTT593820.4772909818432184No Hit
TAGCTTATCAGACTGATGTTGA559960.4500755417347488No Hit
AAACCGTTACCATTACTGAG536560.4312674703071591No Hit
CCCATAAAGTAGAAAGCACT460320.3699885230576105No Hit
TGAGGGGCAGAGAGCGAGACT443850.35675053432203774No Hit
TACAGTATAGATGATGTACT420020.3375968444878727No Hit
TTCAAGTAATCCAGGATAGGCC415260.333770929103457No Hit
CGCGACCTCAGATCAGACGT412640.331665068114556No Hit
CATTGCACTTGTCTCGGTCTGA391910.3150030458626784No Hit
TCGTACCGTGAGTAATAATGCG375010.3014194387205303No Hit
TAGCTTATCAGACTGATGTTG369910.29732024366579923No Hit
TTCAAGTAATTCAGGATAGGT352660.2834553192159735No Hit
TCCTGTACTGAGCTGCCCCGAGA335270.2694778678430767No Hit
TGAGGTAGTAGGTTGTATAGC329360.264727624162006No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC327060.2628789675686959No Hit
TATTGCACTTGTCCCGGCCTGC314740.25297659833844355No Hit
TCCTGTACTGAGCTGCCCCGAGT302620.24323498185543552No Hit
TGAGGTAGTAGTTTGTACAGTT300630.24163549202035417No Hit
TCAGTGCACTACAGAACTTTGC290500.23349336537242757No Hit
TGAGGTAGTAGGTTGTATAG288280.2317090098780152No Hit
TGAGGTAGTAGTTTGTGCTGT287140.23079271921872235No Hit
TGGCTCAGTTCAGCAGGAAC278050.22348650685646634No Hit
TGAGAACTGAATTCCATGGGTT278030.22347043158174193No Hit
TGTAAACATCCCCGACTGGAAGCT276410.22216833432906263No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC271880.21852728460397797No Hit
CGCGACCTCAGATC269870.2169117194941722No Hit
TCCTGTACTGAGCTGCCCCG258380.20767647416498392No Hit
AAACCGTTACCATTACTG256300.2060046455936426No Hit
TGAGGTAGTAGATTGTATAGC239710.19267020520972325No Hit
AAACCGTTACCATTACTGAGTT223190.17939202828733944No Hit
ATTTTAGCGGAACGCTCTGGAAAGTGC220550.1772700920237139No Hit
TGAGGTAGTAGGTTGTATAGTC214680.17255199889209205No Hit
TGAGGTAGTAGGTTGTAT209870.16868589532086528No Hit
TGAGGTAGTAGTTTGTGCTGTC209350.16826793817802999No Hit
TGAGGTAGTAGGTTGTGTGGC208750.1677856799362969No Hit
TGAGGTAGTAGGTTGTGTGG205040.1648037164749141No Hit
TGAGGTAGTAGATTGTATAG204090.1640401409255034No Hit
TGAGGTAGTAGATTGTAT203590.16363825905739252No Hit
AAAAGCTGGGTTGAGAGGGCGA202080.16242457581569764No Hit
TGGGTGTCAGAGCAAACCAGGACGT201500.161958392848689No Hit
AAACCGTTACCATTACTGAGA196660.15806817636537557No Hit
TGAGGTAGTAGATTGTATAGTC195540.15716796098080718No Hit
TTCACAGTGGCTAAGTTCCG193660.15565688515671022No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT187040.15033596922292203No Hit
TGAGGTAGTAGGTTGTGTGGTC181050.14552142444295355No Hit
CAACGGAATCCCAAAAGCAGCT175710.14122932609152924No Hit
TGTAAACATCCTTGACTGGAAGCT174980.14064257856408732No Hit
AAGCTGCCAGTTGAAGAACTGC173190.13920384147625037No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT171260.13765257746534232No Hit
AAGCTGCCAGTTGAAGAACT171090.1375159376301846No Hit
CTAGACTGAAGCTCCTTGAGG170150.13676039971813614No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT169590.13631029202585193No Hit
GGGTGTCAGAGCAAACCAGGACGT167210.1343973343336441No Hit
TACAGTACTGTGATAACTGAAG154370.12407700796055642No Hit
CATTGCACTTGTCTCGGTCTG153970.12375550246606772No Hit
GGGTGTCAGAGCAAACCAGGACG151740.12196310933429313No Hit
ACCAATATTACTGTGCTGCTT151240.12156122746618224No Hit
TAGCTTATCAGACTGATGTTGAT150650.1210870068618114No Hit
AGAGGTAGTAGGTTGCATAGT150490.1209584046640159No Hit
TGGGTGTCAGAGCAAACCAGGACG149280.11998585054318754No Hit
AACCCGTAGATCCGATCTTGT147130.11825775851031073No Hit
TGAGGGGCAGAGAGCGAGACTT145450.11690743543345812No Hit
TTCAAGTAATCCAGGATAGGCA143000.11493821427971476No Hit
TGAGGTAGTAGTTTGTACAGC141390.1136441546643977No Hit
CGCGACCTCAGATCAGAC141320.11358789120286218No Hit
TCAGTGCATCACAGAACTTTGT139420.1120607401040408No Hit
TATTGCACTTGTCCCGGCCTG139310.11197232609305638No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG131920.10603251208237742No Hit
AAACCGTTACCATTACTGAGG130530.10491528048902914No Hit
AGCTCGGTCTGAGGCCCCTCAGT129640.10419993076379176No Hit
TGGAGTGTGACAATGGTGTTC129350.10396683928028745No Hit
TGGGTGTCAGAGCAAACCAGGACGTT124420.10000428406071406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)