FastQCFastQC Report
Thu 9 May 2024
EGAF00002167688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9362468
Total Bases214.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT109270311.671099970648765No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3734993.989322046280959No Hit
TCAGTGCACTACAGAACTTTGT2975103.1776877635256002No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1742061.8606845972664474No Hit
TCCTGTACTGAGCTGCCCCGAG1526991.6309695264111985No Hit
TGGAGTGTGACAATGGTGTTT1180881.2612913603549833No Hit
TTCAAGTAATCCAGGATAGGCT1051841.1234644540307106No Hit
TGAGGTAGTAGGTTGTGTGGT1030781.1009703851591268No Hit
TCCTGTACTGAGCTGCCCCGA1005281.0737339769812833No Hit
TGAGGTAGTAGGTTGTATAGT997831.0657766734155993No Hit
AAACCGTTACCATTACTGAGC930980.994374560212115No Hit
TATTGCACTTGTCCCGGCCTGT885390.9456801347678838No Hit
CGCGACCTCAGATCAGA882410.9424972133416103No Hit
TAGCTTATCAGACTGATGTTGAC821690.8776425190451919No Hit
TGAGGTAGTAGGTTGTGTGGTT766270.8184487252720116No Hit
TGAGGTAGTAGATTGTATAGT762360.8142724760180755No Hit
TAGCTTATCAGACTGATGTTGA743670.7943097909653738No Hit
AAGCTGCCAGTTGAAGAACTGT727930.7774979845057949No Hit
TCGTACCGTGAGTAATAATGCG726990.7764939757337488No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC712980.76152997265251No Hit
TGGCTCAGTTCAGCAGGAAC660080.7050277768639636No Hit
TGAGGGGCAGAGAGCGAGACTTT652610.697049111409513No Hit
TGAGGTAGTAGGTTGTATAGTT592760.6331236592744562No Hit
TGAGGTAGTAGTTTGTGCTGTT581530.6211289587318216No Hit
AAACCGTTACCATTACTGAG579380.6188325556893759No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC544040.5810860982381996No Hit
TGAGGGGCAGAGAGCGAGACT535870.5723597666769061No Hit
TTCACAGTGGCTAAGTTCCG535180.5716227815144468No Hit
TGAGGTAGTAGATTGTATAGTT528220.5641888442235531No Hit
CATTGCACTTGTCTCGGTCTGA523650.559307652640308No Hit
CGCGACCTCAGATCAGACGT500050.5341006238953233No Hit
TAGCTTATCAGACTGATGTTG483650.5165838751064357No Hit
TGGCTCAGTTCAGCAGGAACAG475490.5078682244895256No Hit
TGAGGTAGTAGTTTGTACAGT465520.4972193229392079No Hit
TGAGGTAGTAGGTTGTGTGG459860.49117390841816494No Hit
TCCTGTACTGAGCTGCCCCGAGA457830.4890056767083209No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT439220.46912843921068675No Hit
CTAGACTGAAGCTCCTTGAGG417700.4461430468974634No Hit
AAACCGTTACCATTACTG374820.4003431573811521No Hit
AAAAGCTGGGTTGAGAGGGCGA373270.3986876110017145No Hit
CCCATAAAGTAGAAAGCACT336740.35967012116890545No Hit
TGAGGTAGTAGGTTGTATAG333130.3558143002464735No Hit
TCCTGTACTGAGCTGCCCCG329610.3520546078234927No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG277950.29687684913849643No Hit
TCAGTGCACTACAGAACTTTGC271520.29000900189992634No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC249340.2663186672573941No Hit
TCCTGTACTGAGCTGCCCCGAGT234570.2505429124030117No Hit
TGGCTCAGTTCAGCAGGAACA230530.24622781087209056No Hit
TGTAAACATCCCCGACTGGAAG226250.24165636667596624No Hit
CATTGCACTTGTCTCGGTCTG225780.2411543622899432No Hit
TGAGGTAGTAGATTGTATAG224400.2396803919650246No Hit
TACAGTACTGTGATAACTGAAG212130.22657487320650924No Hit
TTCAAGTAATTCAGGATAGGT206570.22063626812930093No Hit
TGAGAACTGAATTCCATGGGTT204790.2187350600290436No Hit
TGAGATGAAGCACTGTAGCTC203580.217442665758644No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT197730.21119431329431515No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC189930.20286317667521No Hit
TGTAAACATCCCCGACTGGAAGCT170460.18206737796059758No Hit
TGAGGTAGTAGTTTGTGCTGT169970.18154401168580764No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC167480.17888445653432408No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT165700.17698324843406674No Hit
TGAGGTAGTAGTTTGTACAGTT165020.1762569442159909No Hit
TACAGTATAGATGATGTACT164240.1754238305540804No Hit
TGTAAACATCCCCGACTGGAAGC156320.16696452260237363No Hit
AAAAGCTGGGTTGAGAGGGCG155500.16608868516292927No Hit
CGTGTATTTGACAAGCTGAGTTG150840.16111136508023313No Hit
CTAGACTGAAGCTCCTTGAGGA143010.1527481856279776No Hit
TGTAAACATCCTTGACTGGAAGCT142640.15235299068578925No Hit
TGAGGGGCAGAGAGCGAGACTT141420.15104991547100613No Hit
TAGCAGCACGTAAATATTGGCG135750.14499382000557973No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA133320.14239835052039698No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT133010.14206724124450945No Hit
TGAGATGAAGCACTGTAGCT129930.13877751037440128No Hit
TGGAGAGAAAGGCAGTTCCTG127580.13626748844428627No Hit
TAGCAGCACGTAAATATTGGC127020.13566935555881207No Hit
AAACCGTTACCATTACTGAGTT126200.13479351811936768No Hit
TATTGCACTTGTCCCGGCCTG125940.1345158135653975No Hit
AAATGGATTTTTGGAGCAGGGAGA118710.12679349077615007No Hit
TTCAAGTAATCCAGGATAGGCC116220.12413393562466649No Hit
TTCACAGTGGCTAAGTTCCGA115030.12286290324303378No Hit
TCGTACCGTGAGTAATAATGC112750.12042764792360304No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT112500.12016062431401633No Hit
AGCTCGGTCTGAGGCCCCTCAGT111140.11870801587786468No Hit
AAGCTGCCAGTTGAAGAACT109690.11715927894226182No Hit
TGGAGTGTGACAATGGTGTTTG108420.11580279900556135No Hit
TGGAGAGAAAGGCAGTTCCTGA108240.11561054200665893No Hit
TGAGGTAGTAGTTTGTGCT108030.1153862421746061No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA104830.1119683399718963No Hit
TACAGTACTGTGATAACTGAA101650.10857179965795344No Hit
AACCCGTAGATCCGATCTTGT101580.10849703304726915No Hit
TCAGTGCATCACAGAACTTTGT101360.10826205227083285No Hit
CGCGACCTCAGATC100550.10739689577577195No Hit
ACCAATATTACTGTGCTGCTT99160.10591224450646987No Hit
TGAGGTAGTAGTTTGTGCTG94520.10095628631254068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)