FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12630782
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10557458.358508602238562No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4628513.6644682807446127No Hit
TCAGTGCACTACAGAACTTTGT3642712.883994039323931No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2479061.9627129975008675No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1907251.510001518512472No Hit
TTCAAGTAATCCAGGATAGGCT1391161.101404489444913No Hit
TCCTGTACTGAGCTGCCCCGAG1374271.0880323957772369No Hit
TGAGGTAGTAGGTTGTATAGT1255560.9940477161271567No Hit
TAGCTTATCAGACTGATGTTGA1217600.9639941533311239No Hit
TAGCTTATCAGACTGATGTTGAC1144380.9060246626060049No Hit
AAACCGTTACCATTACTGAGC1063770.8422043860783917No Hit
TATTGCACTTGTCCCGGCCTGT978220.7744730294608837No Hit
TGAGGTAGTAGATTGTATAGT974070.7711874054987252No Hit
TGAGGTAGTAGGTTGTATAGTT927370.734214239466725No Hit
TTCACAGTGGCTAAGTTCCG878830.6957843148587316No Hit
AAACCGTTACCATTACTGAG876660.6940662897990005No Hit
TGAGGTAGTAGTTTGTGCTGTT831870.6586053025062106No Hit
TCCTGTACTGAGCTGCCCCGA815750.6458428306339228No Hit
CATTGCACTTGTCTCGGTCTGA798980.632565742960333No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC786110.6223763500945547No Hit
TCGTACCGTGAGTAATAATGCG778420.6162880493068442No Hit
TGAGGTAGTAGATTGTATAGTT775330.6138416449591165No Hit
TGGCTCAGTTCAGCAGGAAC721440.5711760364480996No Hit
AAGCTGCCAGTTGAAGAACTGT694090.5495225869625491No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC684760.5421358709223228No Hit
CGCGACCTCAGATCAGA674710.5341791189175776No Hit
TAGCTTATCAGACTGATGTTG672690.5325798513504547No Hit
TGAGGTAGTAGTTTGTACAGT660580.5229921631138912No Hit
TGGAGTGTGACAATGGTGTTT645120.5107522242090791No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT628630.4976968171883578No Hit
AGGAAACTCTGGTG594060.47032717372526894No Hit
CCCATAAAGTAGAAAGCACT590090.4671840587542403No Hit
TGAGGTAGTAGGTTGTGTGGT572860.4535427814366521No Hit
TGAGGTAGTAGGTTGTGTGGTT559970.44333755423852617No Hit
TGAGGTAGTAGGTTGTATAG505700.4003710934129019No Hit
CGCGACCTCAGATC468580.37098257257547473No Hit
TGAGGGGCAGAGAGCGAGACTTT465900.368860772040876No Hit
TCCTGTACTGAGCTGCCCCGAGA460550.36462508813785244No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC453600.35912265764700874No Hit
CGCGACCTCAGATCAGACGT445360.352598912719735No Hit
TCAGTGCACTACAGAACTTTGC402250.3184680093441562No Hit
AAAAGCTGGGTTGAGAGGGCGA378880.2999655919958083No Hit
TGAGGTAGTAGATTGTATAG370170.29306974025836247No Hit
AAACCGTTACCATTACTG369520.29255512445706056No Hit
TGGCTCAGTTCAGCAGGAACAG362680.28713978279412944No Hit
TACAGTATAGATGATGTACT340350.26946075072786463No Hit
CATTGCACTTGTCTCGGTCTG337620.26729936436239654No Hit
AAACCGTTACCATTACTGAGTT327390.25920010336652155No Hit
TGAGGTAGTAGTTTGTACAGTT321740.2547269044782817No Hit
TACAGTACTGTGATAACTGAAG312220.2471897622807519No Hit
TGTAAACATCCCCGACTGGAAG304580.24114104732391073No Hit
TAGCAGCACGTAAATATTGGCG304020.2406976860181737No Hit
TGAGGGGCAGAGAGCGAGACT301490.23869464297618312No Hit
TTCAAGTAATTCAGGATAGGT300300.23775250020149188No Hit
TGAGGTAGTAGTTTGTGCTGT299140.23683410892532228No Hit
TGAGATGAAGCACTGTAGCTC295650.23407101793063959No Hit
TGAGGTAGTAGGTTGTGTGG294820.2334138931382079No Hit
TCCTGTACTGAGCTGCCCCG291760.2309912402890019No Hit
TCCTGTACTGAGCTGCCCCGAGT278740.22068308993061556No Hit
CTAGACTGAAGCTCCTTGAGG265250.21000283276205703No Hit
TGAGATGAAGCACTGTAGCT259840.20571964586199018No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT255700.2024419390660056No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG235460.18641759473008085No Hit
TGAGAACTGAATTCCATGGGTT230150.18221357949175276No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC228290.18074098658341187No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC224520.17775621493586066No Hit
TGAAAAGAACTTTGAAGAGAG212160.1679705975449501No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC210430.16660092779686958No Hit
TGGCTCAGTTCAGCAGGAACA209130.16557169619426573No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT207670.16441578993287986No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG206230.16327571800384172No Hit
TGTAAACATCCTTGACTGGAAGCT203340.1609876569795916No Hit
GTTTCCGTAGTGTAGTGGTTATC193240.152991319143977No Hit
TAGCAGCACGTAAATATTGGC186000.14725929083409087No Hit
TTCAAGTAATCCAGGATAGGCC179500.14211313282107157No Hit
CGTGTATTTGACAAGCTGAGTTG172340.13644444184057644No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT170090.1346630794514544No Hit
TACAGTACTGTGATAACTGAA167670.13274712523737645No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA164020.12985735958391176No Hit
TGAGGTAGTAGTTTGTGCTG161370.12775931054783463No Hit
TGGAGAGAAAGGCAGTTCCTGA158010.12509914271341235No Hit
TTCACAGTGGCTAAGTTCCGA156540.12393531928585261No Hit
TGTAAACATCCCCGACTGGAAGCT156190.12365821846976695No Hit
TACAGTACTGTGATAACTGA154760.12252606370690271No Hit
TGGAGAGAAAGGCAGTTCCTG152870.12102971930004017No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT148440.11752241468501318No Hit
TCAGTGCATCACAGAACTTTGT148400.11749074602031766No Hit
CAACGGAATCCCAAAAGCAGCTG147920.11711072204397162No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA147500.11677820106466884No Hit
GCATTGGTGGTTCAGTGGTAGAATT142750.11301754713207782No Hit
GCATTGTGGTTCAGTGGTAGAATTCTC141340.11190122670156132No Hit
TGTAAACATCCCCGACTGGAAGC139910.11076907193869708No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA139700.11060281144904568No Hit
CGCGACCTCAGATCAGACG139450.11040488229469878No Hit
GGCTGGTCCGATGGTAGTGGGTTATC136610.10815640710131803No Hit
TGAGGTAGTAGGTTGTATAGC136380.10797431227931888No Hit
TGTCAGTTTGTCAAATACCCCAA136110.10776054879262426No Hit
TGAGGTAGTAGTTTGTACAG134830.10674715152236813No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA134150.10620878422254458No Hit
AACATTCAACGCTGTCGGTGA133830.10595543490498055No Hit
TGAGGTAGTAGGTTGTAT133120.10539331610663535No Hit
AAAAGCTGGGTTGAGAGGGCG132030.1045303449936829No Hit
TGTCAGTTTGTCAAATACCCCA130910.1036436223822088No Hit
TGAGGGGCAGAGAGCGAGACTT130120.10301816625447262No Hit
TGAGGTAGTAGATTGTAT129480.10251146761934456No Hit
AGCTCGGTCTGAGGCCCCTCAGT129120.10222644963708502No Hit
AGGAAACTCTGGTGG127190.10069843656552697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)