FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10383327
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8361138.052457560086474No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4811034.63341855649928No Hit
TCAGTGCACTACAGAACTTTGT2371172.283632211525265No Hit
CGCGACCTCAGATCAGA2195262.1142163778526863No Hit
TCCTGTACTGAGCTGCCCCGAG1610551.5510924388685823No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1223971.1787840255825517No Hit
AAACCGTTACCATTACTGAG1090941.0506651673399094No Hit
TTCAAGTAATCCAGGATAGGCT971050.9352012124822805No Hit
TATTGCACTTGTCCCGGCCTGT932420.8979973374622604No Hit
TCCTGTACTGAGCTGCCCCGA905680.8722445127655134No Hit
TGGAGTGTGACAATGGTGTTT828880.7982797806521936No Hit
AAACCGTTACCATTACTGAGC801400.7718142749428964No Hit
TGAGGTAGTAGGTTGTATAGT748330.7207034893536532No Hit
TAGCTTATCAGACTGATGTTGAC719150.6926007434803894No Hit
TGAGGGGCAGAGAGCGAGACTTT716140.6897018653077188No Hit
TGAGGTAGTAGGTTGTATAGTT687980.6625814635328349No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT666080.641489957891146No Hit
TAGCTTATCAGACTGATGTTGA655710.6315027928909491No Hit
AAGCTGCCAGTTGAAGAACTGT559870.5392009709412022No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC556680.536128737927641No Hit
TGGCTCAGTTCAGCAGGAAC533570.5138719025221877No Hit
TTCACAGTGGCTAAGTTCCG531730.5120998308153061No Hit
CGCGACCTCAGATC519970.500773981210454No Hit
CATTGCACTTGTCTCGGTCTGA492380.47420253643172366No Hit
AAATGGATTTTTGGAGCAGGGAGA470910.45352515624327344No Hit
TGAGGTAGTAGATTGTATAGT466790.44955725655177764No Hit
TGAGGTAGTAGATTGTATAGTT462440.4453678478969217No Hit
TGAGGTAGTAGGTTGTGTGGT446230.4297562813922744No Hit
TGAGGTAGTAGGTTGTGTGGTT443660.42728115949733647No Hit
TGAGGTAGTAGGTTGTATAG427800.4120066718499764No Hit
TAGCTTATCAGACTGATGTTG426620.4108702345596936No Hit
TCGTACCGTGAGTAATAATGCG423880.408231388648359No Hit
TCCTGTACTGAGCTGCCCCGAGA409540.3944207863240751No Hit
CGCGACCTCAGATCAGACGT401840.3870050514637553No Hit
TGAGGGGCAGAGAGCGAGACT388840.3744849796216569No Hit
CCCATAAAGTAGAAAGCACT372120.3583822410678196No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG365110.35163103309758037No Hit
TCCTGTACTGAGCTGCCCCG361620.3482698753492016No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC360240.34694082156904044No Hit
TGAGGTAGTAGTTTGTGCTGTT353110.34007404370487415No Hit
AAATGGATTTTTGGAGCAGGGAG338630.32612860983767533No Hit
AAAAGCTGGGTTGAGAGGGCGA334250.32191030870933757No Hit
AAACCGTTACCATTACTG334030.32169843057047126No Hit
TGAGGTAGTAGTTTGTACAGT320750.3089086956425431No Hit
AAATGGATTTTTGGAGCAGGGA288530.2778781791231269No Hit
TGAGGTAGTAGGTTGTGTGG270910.2609086663648366No Hit
TGTAAACATCCCCGACTGGAAG266330.2564977487466204No Hit
TGGCTCAGTTCAGCAGGAACAG240600.23171763732375952No Hit
TCAGTGCACTACAGAACTTTGC239710.23086049394380048No Hit
TGAGGTAGTAGATTGTATAG225590.21726176975838282No Hit
AAATGGATTTTTGGAGCAGGG218800.21072243992700992No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT210240.20247845416021282No Hit
CATTGCACTTGTCTCGGTCTG204580.19702740749665304No Hit
TCCTGTACTGAGCTGCCCCGAGT196530.18927459377904596No Hit
CGCGACCTCAGATCAGAC193630.18648165467580863No Hit
TATTGCACTTGTCCCGGCCTG192440.1853355865610319No Hit
TGAGATGAAGCACTGTAGCTC192060.1849696152302629No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC191200.18414136432378564No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA189510.18251375498431283No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA182780.17603221009990344No Hit
TTCAAGTAATTCAGGATAGGT180080.17343188748654453No Hit
TGAGGGGCAGAGAGCGAGACTT179280.1726614215270308No Hit
TGTAAACATCCCCGACTGGAAGC173780.16736446805537378No Hit
AAACCGTTACCATTACTGAGTT172610.16623766158958492No Hit
TGTAAACATCCCCGACTGGAAGCT169470.16321358269849345No Hit
CTAGACTGAAGCTCCTTGAGG162920.15690539265497466No Hit
TGAGAACTGAATTCCATGGGTT161670.1557015395932344No Hit
TGAAAAGAACTTTGAAGAGAG153530.14786204845518205No Hit
TGTAAACATCCTTGACTGGAAGCT150650.14508837100093255No Hit
TACAGTACTGTGATAACTGAAG148540.14305626703271504No Hit
CGCGACCTCAGATCAGACG147190.1417561057260356No Hit
GGCTGGTCCGATGGTAGTGGGTTATC145510.14013812721105673No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC144480.13914615228818278No Hit
CGTGTATTTGACAAGCTGAGTTG141540.13631468988696976No Hit
AAAAGCTGGGTTGAGAGGGCG140290.1351108368252295No Hit
TGAGATGAAGCACTGTAGCT135830.13081548910094037No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT135100.1301124389128841No Hit
TGAGGTAGTAGTTTGTACAGTT131440.12658755714810868No Hit
TGGAGTGTGACAATGGTGTTTG126590.12191660726855659No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG122090.11758273624629177No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC122040.11753458212382217No Hit
AAATGGATTTTTGGAAATAGGAGA121600.1171108258460896No Hit
TGAGGTAGTAGTTTGTGCTGT121330.11685079358475371No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG118840.11445271828576717No Hit
CAACGGAATCCCAAAAGCAGCTG118730.11434677921633403No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA118160.11379782222018049No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC116470.1121702128807077No Hit
TTCAAGTAATCCAGGATAGGCC116000.11171756412949335No Hit
TGGCTCAGTTCAGCAGGAACA112840.10867422358941407No Hit
TAGCAGCACGTAAATATTGGCG111710.1075859404216009No Hit
TGTAAACATCCCCGACTGGAA109410.10537085078799888No Hit
AAATGGATTTTTGGAGCAGGGAGAT107320.1033580084687692No Hit
TAGCAGCACGTAAATATTGGC105780.10187486149670524No Hit
AGCTCGGTCTGAGGCCCCTCAGT104630.10076731667990424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)