FastQCFastQC Report
Wed 8 May 2024
EGAF00002167694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198162
Total Bases258.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9214688.22874325268736No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5892245.261792069091338No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1801431.6086836393329549No Hit
TCCTGTACTGAGCTGCCCCGAG1650931.4742865838161656No Hit
TCAGTGCACTACAGAACTTTGT1625011.451139928141779No Hit
TCCTGTACTGAGCTGCCCCGA1610911.4385485760966845No Hit
TTCAAGTAATCCAGGATAGGCT1519081.3565440471391643No Hit
AAACCGTTACCATTACTGAGC1428541.275691492943217No Hit
AAACCGTTACCATTACTGAG1189461.0621921704651174No Hit
TAGCTTATCAGACTGATGTTGAC1175811.0500026700810365No Hit
TATTGCACTTGTCCCGGCCTGT1153431.0300172474732907No Hit
TGAGGTAGTAGGTTGTATAGT1048210.9360553990913866No Hit
TGAGGGGCAGAGAGCGAGACTTT1027080.9171862310975677No Hit
CGCGACCTCAGATCAGA942770.8418970898974313No Hit
TGGAGTGTGACAATGGTGTTT845240.7548024399004051No Hit
TGAGGTAGTAGGTTGTATAGTT816920.7295125753672791No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT791890.7071606929780084No Hit
TAGCTTATCAGACTGATGTTGA735430.6567417045761617No Hit
AAGCTGCCAGTTGAAGAACTGT732790.6543841748315482No Hit
TGAGGTAGTAGTTTGTGCTGTT724510.6469901042688969No Hit
TGAGGTAGTAGATTGTATAGTT719780.6427661968097979No Hit
TGAGGTAGTAGATTGTATAGT711230.6351310152505384No Hit
TGGCTCAGTTCAGCAGGAAC633230.5654767273415048No Hit
TGAGGGGCAGAGAGCGAGACT607630.5426158328482835No Hit
TAGCTTATCAGACTGATGTTG589330.5262738653003948No Hit
TGAGGTAGTAGGTTGTGTGGT585550.5228983113478801No Hit
TGAGGTAGTAGGTTGTGTGGTT549420.4906340879869393No Hit
TCCTGTACTGAGCTGCCCCG546910.48839264872217425No Hit
TGAGGTAGTAGTTTGTACAGT544650.48637446038019455No Hit
TTCACAGTGGCTAAGTTCCG521580.46577286522556116No Hit
TCGTACCGTGAGTAATAATGCG479070.42781127831513777No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG468960.41878301099769766No Hit
CCCATAAAGTAGAAAGCACT418030.37330233300786325No Hit
TCCTGTACTGAGCTGCCCCGAGA406770.36324711144561045No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC394020.3518613143835569No Hit
AAATGGATTTTTGGAGCAGGGAGA386090.3447797951128051No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC365100.32603564763574594No Hit
CGCGACCTCAGATCAGACGT361390.3227226039416111No Hit
AAAAGCTGGGTTGAGAGGGCGA356210.3180968448214984No Hit
AAACCGTTACCATTACTG342750.30607701513873437No Hit
TGAGGTAGTAGGTTGTGTGG342090.305487632702581No Hit
TGAGGTAGTAGGTTGTATAG341830.3052554517428842No Hit
GTTTCCGTAGTGTAGTGGTTATC313840.28026027842783485No Hit
CATTGCACTTGTCTCGGTCTGA302160.26982999531530266No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC300770.268588720184616No Hit
TTCAAGTAATCCAGGATAGGCC294900.26334678851761567No Hit
TGTAAACATCCCCGACTGGAAG292030.2607838679240397No Hit
TGTAAACATCCCCGACTGGAAGCT288740.25784588578018425No Hit
TCAGTGCACTACAGAACTTTGC272500.24334350583604702No Hit
TTCAAGTAATTCAGGATAGGT269970.24108420649745915No Hit
CGCGACCTCAGATC266300.23780688295096997No Hit
TATTGCACTTGTCCCGGCCTG264180.23591371512575013No Hit
AAATGGATTTTTGGAGCAGGG250970.22411713636577146No Hit
TGAGAACTGAATTCCATGGGTT250790.22395639570136602No Hit
TCCTGTACTGAGCTGCCCCGAGT235700.2104809700020414No Hit
AAATGGATTTTTGGAGCAGGGAG226830.20256002726161668No Hit
AAATGGATTTTTGGAGCAGGGA218770.19536241751101652No Hit
TGTAAACATCCTTGACTGGAAGCT214570.19161180200822242No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA214200.19128139064250008No Hit
TGAGGGGCAGAGAGCGAGACTT211910.18923641218978615No Hit
TATTGCACTTGTCCCGGCCTGC208490.18618233956608235No Hit
TGAGGTAGTAGTTTGTACAGTT206090.18403913070734285No Hit
TACAGTACTGTGATAACTGAAG205900.18386946000602597No Hit
TGAGGTAGTAGATTGTATAG201730.1801456346139661No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA200340.17890435948327948No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT199670.1783060470102147No Hit
AAACCGTTACCATTACTGAGTT189080.16884913792102668No Hit
CATTGCACTTGTCTCGGTCTG185740.1658665055926142No Hit
TGTAAACATCCCCGACTGGAAGC185410.16557181437453755No Hit
TGAGGTAGTAGTTTGTGCTGT179900.1606513640363481No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT179860.16061564388870245No Hit
TGAGATGAAGCACTGTAGCTC176150.15730260019456765No Hit
CAACGGAATCCCAAAAGCAGCTG174810.15610597524843808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT167710.14976564904133374No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT166590.1487654849072553No Hit
AAGCTGCCAGTTGAAGAACT166550.14872976475960967No Hit
TGGCTCAGTTCAGCAGGAACAG166070.14830112298786177No Hit
AAAAGCTGGGTTGAGAGGGCG164850.1472116584846692No Hit
TGAGGTAGTAGGTTGTATAGC164630.14701519767261806No Hit
CTAGACTGAAGCTCCTTGAGG154750.13819232120414046No Hit
CAACGGAATCCCAAAAGCAGCT154360.1378440497645953No Hit
GCATTGGTGGTTCAGTGGTAGAATT148300.13243244739627807No Hit
GGCTGGTCCGATGGTAGTGGGTTATC146540.1308607608998691No Hit
AGCTCGGTCTGAGGCCCCTCAGT142120.12691368458502386No Hit
AACCCGTAGATCCGATCTTGT140620.12557417904831167No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC133940.11960891439148674No Hit
TACAGTATAGATGATGTACT131940.11782290700920384No Hit
TGAGGTAGTAGGTTGTAT131430.11736747512672169No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA128620.11485813475461419No Hit
TGAGGTAGTAGATTGTAT128610.11484920471770278No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA127580.11392941091582709No Hit
AAGCTGCCAGTTGAAGAACTGC125090.11170583172488485No Hit
AAATGGATTTTTGGAGCAGGGAGAT125060.11167904161415061No Hit
TGTAAACATCCCCGACTGGAA123500.11028595585596994No Hit
GTAGTCGTGGCCGAGTGGTTAAGG122340.10925007157424585No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC119920.10708900264168351No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC119440.10666036086993563No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT118020.10539229562851475No Hit
TGAGATGAAGCACTGTAGCT117860.10524941503793211No Hit
TACAGTACTGTGATAACTGA117190.10465110256486734No Hit
AGCAGCATTGTACAGGGCTATG116750.10425818094076511No Hit
TGAGGTAGTAGATTGTATAGC115060.10274900470273604No Hit
CGCGACCTCAGATCAGAC113910.10172205045792335No Hit
TGGAGTGTGACAATGGTGTTTG113550.10140056912911243No Hit
TGAGGTAGTAGGTTGTATAGTC113470.10132912883382113No Hit
CGCGACCTCAGATCAGACG113430.10129340868617547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)