FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11199297
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT162931214.548341739664552No Hit
TCAGTGCACTACAGAACTTTGT3529043.1511263608778304No Hit
AAACCGTTACCATTACTGAGC2546892.2741516721987103No Hit
TGAGGTAGTAGGTTGTATAGT1931401.7245725334366968No Hit
TGAGGTAGTAGGTTGTATAGTT1556941.3902122606445744No Hit
TTCAAGTAATCCAGGATAGGCT1547481.3817653018756446No Hit
CGCGACCTCAGATCAGA1484561.3255832040171807No Hit
TATTGCACTTGTCCCGGCCTGT1470141.312707395830292No Hit
TCCTGTACTGAGCTGCCCCGAG1461711.3051801376461396No Hit
TGGAGTGTGACAATGGTGTTT1254541.120195312259332No Hit
TCCTGTACTGAGCTGCCCCGA1198241.069924299712741No Hit
TGAGGTAGTAGATTGTATAGT1090700.9739004153564281No Hit
TGAGGTAGTAGGTTGTGTGGT1088230.9716949197793397No Hit
TGAGGTAGTAGATTGTATAGTT1005820.8981099438652265No Hit
AAGCTGCCAGTTGAAGAACTGT864000.7714769953864068No Hit
TGAGGTAGTAGGTTGTGTGGTT849710.7587172659141016No Hit
TGAGGGGCAGAGAGCGAGACTTT712710.6363881590067663No Hit
TGAGGGGCAGAGAGCGAGACT700460.6254499724402344No Hit
CATTGCACTTGTCTCGGTCTGA637470.5692053706585333No Hit
TGAGGTAGTAGTTTGTGCTGTT615830.549882729246309No Hit
AAACCGTTACCATTACTGAG586440.5236400106185236No Hit
CGCGACCTCAGATCAGACGT586390.5235953649590684No Hit
TCAGTGCACTACAGAACTTTGC583310.5208451923366262No Hit
TGAGGTAGTAGTTTGTACAGT533960.4767799264543122No Hit
CCCATAAAGTAGAAAGCACT524330.4681811724432346No Hit
TCGTACCGTGAGTAATAATGCG518100.4626183232751127No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC498290.4449297129989499No Hit
TGAGGTAGTAGGTTGTATAG489170.43678634471431554No Hit
TAGCTTATCAGACTGATGTTGAC474830.4239819695825551No Hit
TGGCTCAGTTCAGCAGGAAC458700.40957927984229725No Hit
TAGCTTATCAGACTGATGTTGA443260.39579270020252166No Hit
TCCTGTACTGAGCTGCCCCGAGA434190.3876939775773426No Hit
TTCACAGTGGCTAAGTTCCG365100.3260026053421032No Hit
TGAGGTAGTAGTTTGTACAGTT359550.32104693714257243No Hit
TGGCTCAGTTCAGCAGGAACAG355280.31723419782509565No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC353230.3154037257874311No Hit
AAAAGCTGGGTTGAGAGGGCGA352520.3147697574231668No Hit
TCCTGTACTGAGCTGCCCCG346960.309805160091745No Hit
TGAGGTAGTAGGTTGTGTGG313490.2799193556524128No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT308530.2754905062344538No Hit
TGAGGTAGTAGGTTGTATAGC307770.2748118922107343No Hit
CTAGACTGAAGCTCCTTGAGG299540.2674632166644031No Hit
TTCAAGTAATCCAGGATAGGCC296930.26513271324084003No Hit
TATTGCACTTGTCCCGGCCTGC292480.2611592495493244No Hit
TCCTGTACTGAGCTGCCCCGAGT283060.2527480073079587No Hit
TTCAAGTAATTCAGGATAGGT281960.2517658027999436No Hit
AAACCGTTACCATTACTGAGA281290.2511675509632435No Hit
AAACCGTTACCATTACTG281190.25107825964433306No Hit
TAGCTTATCAGACTGATGTTG277690.2479530634824668No Hit
TGAGGTAGTAGGTTGTATAGTC264020.2357469401874064No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG246310.21993344760836328No Hit
TGAGAACTGAATTCCATGGGTT236500.21117396922324677No Hit
TGAGGTAGTAGTTTGTGCTGT218960.19551227188635145No Hit
AAACCGTTACCATTACTGAGTT213760.19086912330300732No Hit
CGCGACCTCAGATC207090.1849133923316794No Hit
CATTGCACTTGTCTCGGTCTG206060.1839936917469016No Hit
TGAGGTAGTAGATTGTATAG199610.17823440167717672No Hit
TGTAAACATCCCCGACTGGAAGCT195900.1749216937455985No Hit
TGAGGTAGTAGGTTGTAT193390.17268048164094585No Hit
TGGCTCAGTTCAGCAGGAACA193150.17246618247556075No Hit
AGAGGTAGTAGGTTGCATAGT189490.16919812020343777No Hit
TGAGGTAGTAGGTTGTGTGGC189140.16888560058725116No Hit
TGAGGGGCAGAGAGCGAGACTT188750.16853736444350034No Hit
TGGAGTGTGACAATGGTGTTC187980.16784982128788978No Hit
TCCTGTACTGAGCTGCCCCGAA186040.16611756970102676No Hit
TGAGATGAAGCACTGTAGCT183900.16420673547634285No Hit
TGAGATGAAGCACTGTAGCTC177870.15882246894604188No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT176700.15777776051478945No Hit
AGAGGTAGTAGGTTGCATAGTT172520.15404538338433207No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT170480.1522238404785586No Hit
TGAGGTAGTAGATTGTATAGC169780.15159880124618536No Hit
TGAGGTAGTAGATTGTATAGTC164500.1468842196077129No Hit
TGTAAACATCCCCGACTGGAAG161580.14427691309552734No Hit
AGACCGTTACCATTACTGAGT160980.14374116518206456No Hit
CAACGGAATCCCAAAAGCAGCT157180.14034809506346693No Hit
AGCTCGGTCTGAGGCCCCTCAGT157050.14023201634888333No Hit
TGAGGTAGTAGGTTGTGTGGTC153990.13749970199022313No Hit
AAACCGTTACCATTACTGAGG153900.1374193398032037No Hit
AAGCTGCCAGTTGAAGAACTGC147750.1319279236902102No Hit
AGGAAACTCTGGTG145770.13015995557578303No Hit
TGTAAACATCCTTGACTGGAAGCT144090.12865986141808722No Hit
CGTGTATTTGACAAGCTGAGTTG143540.1281687591640797No Hit
AAGCTGCCAGTTGAAGAACT138560.12372205148233857No Hit
AAACCGTTACTATTACTGAGT136500.12188265031278303No Hit
TGAGGTAGTAGATTGTAT135140.12066828837560072No Hit
TGGAGAGAAAGGCAGTTCCTGA134360.1199718160880991No Hit
TATTGCACTTGTCCCGGCCTG133950.11960572168056618No Hit
TGAGGTAGTAGTTTGTGCT132950.11871280849146157No Hit
TGGAGTGTGACAATGGTGTTTG131040.1170073443002717No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC131000.11697162777270752No Hit
TCAGTGCATCACAGAACTTTGT129020.11520365965828033No Hit
TTCAAGTAATCCAGGATAGGCA128080.11436432126052198No Hit
TGAGGGGCAGAGAGCGAGACC125410.11198024304561259No Hit
AACCCGTAGATCCGATCTTGT125040.11164986516564387No Hit
TCGTACCGTGAGTAATAATGCA122390.1092836452145166No Hit
CAACGGAATCCCAAAAGCAGCTG119690.10687277960393408No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC119230.10646203953694594No Hit
TACAGTATAGATGATGTACT117280.1047208588181919No Hit
AAAAGCTGGGTTGAGAGGGCG116970.10444405572956947No Hit
TAGCAGCACGTAAATATTGGCG116770.10426547309174852No Hit
TGTAAACATCCCCGACTGGAAGC116020.10359578819992006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)