FastQCFastQC Report
Wed 8 May 2024
EGAF00002167697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10845993
Total Bases234.4 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9790169.026522513890614No Hit
TCAGTGCACTACAGAACTTTGT2361902.1776705922638895No Hit
TTCAAGTAATCCAGGATAGGCT2184952.0145227827456647No Hit
TGAGGTAGTAGGTTGTATAGTT1866341.7207645256639938No Hit
TGAGGTAGTAGGTTGTATAGT1852091.7076260329506023No Hit
AAACCGTTACCATTACTGAGC1520771.4021491623680744No Hit
CGCGACCTCAGATCAGA1412071.3019278179508322No Hit
TGAGGTAGTAGATTGTATAGTT1289911.1892963604162385No Hit
TGGAGTGTGACAATGGTGTTT1239171.1425141063616766No Hit
TGAGGTAGTAGATTGTATAGT1117361.0302053486481138No Hit
TGAGGTAGTAGGTTGTGTGGT1016990.9376642599714013No Hit
TCGTACCGTGAGTAATAATGCG905440.8348152170114806No Hit
TGAGGTAGTAGGTTGTGTGGTT850400.7840683651556846No Hit
TAGCTTATCAGACTGATGTTGAC828540.7639134563335971No Hit
TGAGGGGCAGAGAGCGAGACTTT822220.7580864195652717No Hit
TGAGGTAGTAGTTTGTGCTGTT817840.7540480618049449No Hit
AAGCTGCCAGTTGAAGAACTGT792860.731016514578241No Hit
TCCTGTACTGAGCTGCCCCGAG783260.7221653194871138No Hit
AAACCGTTACCATTACTGAGG781540.7205794803666202No Hit
CGCGACCTCAGATCAGACGT601530.5546103524130985No Hit
TATTGCACTTGTCCCGGCCTGT592540.5463215770100535No Hit
TTCACAGTGGCTAAGTTCCG566390.5222112903816184No Hit
TGAGGGGCAGAGAGCGAGACT554380.5111380765228227No Hit
AAACCGTTACCATTACTGAG528160.4869632499301816No Hit
TGGCTCAGTTCAGCAGGAAC506810.4672785608473101No Hit
TCCTGTACTGAGCTGCCCCGA486790.4488201310843553No Hit
TAGCTTATCAGACTGATGTTGA483000.44532575302233735No Hit
TAGCTTATCAGACTGATGTTG424700.39157318283351283No Hit
TTCAAGTAATCCAGGATAGGCC424250.3911582830636163No Hit
TGAGGTAGTAGGTTGTATAG422270.3893327240760712No Hit
TGAGGTAGTAGTTTGTACAGT421880.3889731442754942No Hit
TCAGTGCACTACAGAACTTTGC391040.36053868004524803No Hit
CTAGACTGAAGCTCCTTGAGG390040.35961668055658896No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC381930.3521392647035638No Hit
CCCATAAAGTAGAAAGCACT331700.305827230388218No Hit
TGAGGTAGTAGTTTGTACAGTT324330.2990320941568006No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT321170.2961185757726379No Hit
AAAAGCTGGGTTGAGAGGGCGA317930.2931312974293825No Hit
TGAGGTAGTAGGTTGTGTGG316990.2922646179100429No Hit
TGGCTCAGTTCAGCAGGAACAG311830.28750710054856204No Hit
TTCAAGTAATCCAGGATAGGCG309890.2857184215405634No Hit
TGAGGTAGTAGGTTGTATAGTC309410.28527586178600706No Hit
TGAGAACTGAATTCCATGGGTT304820.28104388413306186No Hit
TGAGGTAGTAGGTTGTATAGC291860.2690947707600401No Hit
CATTGCACTTGTCTCGGTCTGA246560.22732819392378367No Hit
TTCAAGTAATTCAGGATAGGT241040.22223875674638552No Hit
TACAGTATAGATGATGTACT238070.21950041826506803No Hit
TGAGGTAGTAGATTGTATAG211730.19521495173378778No Hit
TCCTGTACTGAGCTGCCCCG211480.194984451861623No Hit
AGAGGTAGTAGGTTGCATAGTT210360.19395181243432483No Hit
TTCAAGTAATCCAGGATAGGCA209520.1931773328638512No Hit
TCAGTGCACTACAGAACTTTGG206200.19011629456150303No Hit
AAACCGTTACCATTACTG205930.1898673546995651No Hit
TGAGGTAGTAGATTGTATAGTC205370.189351034985916No Hit
TGAGGTAGTAGTTTGTGCTGT197560.18215021897948855No Hit
AAACCGTTACCATTACTGAGA194360.17919982061577946No Hit
TGGAGTGTGACAATGGTGTTC194190.17904308070270744No Hit
TGTAAACATCCCCGACTGGAAGCT193640.17853598098394494No Hit
TGAGGGGCAGAGAGCGAGACTT191860.17689482189413178No Hit
TCCTGTACTGAGCTGCCCCGAGA191010.17611112232877155No Hit
TGAGGTAGTAGGTTGTGTGGC186510.17196212462980567No Hit
TCGTACCGTGAGTAATAATGCA184860.1704408254735182No Hit
TGAGGTAGTAGGTTGTAT177560.1637102292063069No Hit
AGAGGTAGTAGGTTGCATAGT176350.16259460982502938No Hit
TGAGGTAGTAGATTGTATAGC171410.1580399323510535No Hit
TCCTGTACTGAGCTGCCCCGAGT167820.1547299541867674No Hit
AAAAGCTGGGTTGAGAGGGCG162300.14964051700936928No Hit
TGAGGTAGTAGGTTGTGTGGTC158820.14643195878883566No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG154550.14249502097226138No Hit
TAGCTTATCAGACTGATGTTGAT147730.1362069844596064No Hit
TGAGGTAGTAGATTGTAT138160.127383449353139No Hit
TGGAGTGTGACAATGGTGTTTG137780.12703308954744855No Hit
TGAGGTAGTAGTTTGTGCTGTC136890.12621251000254197No Hit
AAGCTGCCAGTTGAAGAACTGC136820.1261479700383358No Hit
TGAGGTAGTAGGTTGTATAGG135330.12477419080023378No Hit
GGTGGGCGTAGCTC132060.12175925247231859No Hit
AGCTCGGTCTGAGGCCCCTCAGT128060.11807125451768224No Hit
CAACGGAATCCCAAAAGCAGCT125590.11579391578069431No Hit
AACCCGTAGATCCGATCTTGT124580.11486269629714864No Hit
TGAGGTAGTAGTTTGTGCT121970.11245627763174842No Hit
AAATGGATTTTTGGAGCAGGGAGA121780.1122810977289032No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT121530.11205059785673842No Hit
TGGCTCAGTTCAGCAGGAACA119730.11039099877715207No Hit
AAAAGCTGGGTTGAGAGGGCGT117680.10850089982540095No Hit
TGGAGAGAAAGGCAGTTCCTGA116200.10713634058218552No Hit
CAACGGAATCCCAAAAGCAGCTG115920.10687818072536097No Hit
AAACCGTTACCATTACTGAGTT115760.10673066080717551No Hit
TATTGCACTTGTCCCGGCCTGC115670.10664768085319619No Hit
CTAGACTGAAGCTCCTTGAGGA114690.1057441213543103No Hit
TTCAAGTAATTCAGGATAGGTT113050.10423204219290941No Hit
CATTGCACTTGTCTCGGTCTG111780.10306110284231237No Hit
TGAGATGAAGCACTGTAGCTC111310.1026277630826426No Hit
TGAGGTAGTAGGTTGTGTGGTTT111020.10236038323093147No Hit
TGTAAACATCCCCGACTGGAAG110860.10221286331274601No Hit
TCGTACCGTGAGTAATAATGC110220.10162278364000418No Hit
TCAGTGCATCACAGAACTTTGT109530.10098660399282942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)