FastQCFastQC Report
Wed 8 May 2024
EGAF00002167698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13198697
Total Bases296 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT182249513.808143334148818No Hit
AAACCGTTACCATTACTGAGC3483422.63921506797224No Hit
TCAGTGCACTACAGAACTTTGT2920452.21268053960175No Hit
TTCAAGTAATCCAGGATAGGCT2272581.721821479802135No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1795241.360164567759984No Hit
TATTGCACTTGTCCCGGCCTGT1520091.1516970197891503No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1390681.053649462518914No Hit
TGGAGTGTGACAATGGTGTTT1329241.0070994129193207No Hit
CGCGACCTCAGATCAGA1103660.8361886025567524No Hit
AAGCTGCCAGTTGAAGAACTGT1039790.7877974621282692No Hit
TCCTGTACTGAGCTGCCCCGA1035650.7846607888642341No Hit
TAGCTTATCAGACTGATGTTGAC1003800.7605296189464763No Hit
TCCTGTACTGAGCTGCCCCGAG993830.7529758429941986No Hit
TGAGGTAGTAGTTTGTGCTGTT974460.7383001519013581No Hit
CGCGACCTCAGATCAGACGT962580.7292992634045617No Hit
TAGCTTATCAGACTGATGTTGA931360.7056454133313311No Hit
TGAGGTAGTAGGTTGTATAGT920690.6975612819962456No Hit
TGAGGTAGTAGGTTGTATAGTT919210.6964399591868804No Hit
TGAGGTAGTAGATTGTATAGTT845720.6407602204975233No Hit
TGAGGTAGTAGTTTGTACAGT776130.5880353189409531No Hit
CCCATAAAGTAGAAAGCACT762740.5778903781183855No Hit
CATTGCACTTGTCTCGGTCTGA756380.5730717206403027No Hit
TGAGGTAGTAGATTGTATAGT707470.5360150323929702No Hit
TGAGGTAGTAGGTTGTGTGGTT701900.5317949188469134No Hit
TGAGGTAGTAGGTTGTGTGGT688910.5219530382430932No Hit
TAGCTTATCAGACTGATGTTG664160.5032011872081009No Hit
AAACCGTTACCATTACTGAG614780.4657884032037405No Hit
TCAGTGCACTACAGAACTTTGC606620.45960597474129455No Hit
CGCGACCTCAGATC605720.458924089249113No Hit
TGAGGGGCAGAGAGCGAGACTTT570020.43187596472591194No Hit
TTCAAGTAATCCAGGATAGGCC542240.4108284325339085No Hit
TCGTACCGTGAGTAATAATGCG477220.3615659939765266No Hit
TTCACAGTGGCTAAGTTCCG466120.35315607290628764No Hit
TGAGGTAGTAGTTTGTGCTGT463880.3514589356813025No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG434910.32950979933852564No Hit
TGAGGGGCAGAGAGCGAGACT425830.32263033237296074No Hit
TGAGGTAGTAGTTTGTACAGTT412660.3126520746707042No Hit
TGGCTCAGTTCAGCAGGAAC405970.3075833925121548No Hit
TATTGCACTTGTCCCGGCCTGC380000.2879072078099831No Hit
TTCAAGTAATTCAGGATAGGT348640.26414728666019077No Hit
TGAGAACTGAATTCCATGGGTT345060.26143489770240197No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC341220.2585255196024274No Hit
TCCTGTACTGAGCTGCCCCGAGT333410.25260826883138543No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT323750.2452893645486369No Hit
TGAGGTAGTAGGTTGTATAG320780.2430391424244378No Hit
AAACCGTTACCATTACTG311450.23597026282215586No Hit
AAAAGCTGGGTTGAGAGGGCGA310750.23543990743934798No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC302630.22928778499877678No Hit
TGAGATGAAGCACTGTAGCT299680.22705271588551504No Hit
TCCTGTACTGAGCTGCCCCG293020.22200676324337165No Hit
TCCTGTACTGAGCTGCCCCGAGA283920.21511214326686945No Hit
AAACCGTTACCATTACTGAGA278010.21063442853487732No Hit
AAACCGTTACCATTACTGAGTT276570.2095434117473869No Hit
TGAGGTAGTAGGTTGTGTGG272670.2065885746146002No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC271100.20539906325601687No Hit
TGTAAACATCCCCGACTGGAAGCT268810.2036640435036883No Hit
TGTAAACATCCTTGACTGGAAGCT268530.20345190135056512No Hit
TACAGTATAGATGATGTACT266170.20166384605995574No Hit
CATTGCACTTGTCTCGGTCTG256500.1943373652717386No Hit
GTTTCCGTAGTGTAGTGGTTATC241040.18262408781715347No Hit
TTCAAGTAATCCAGGATAGGCA234980.17803272550313112No Hit
TGGAGTGTGACAATGGTGTTC233320.1767750255953296No Hit
TACAGTACTGTGATAACTGAAG232540.17618405816877228No Hit
AAGCTGCCAGTTGAAGAACTGC229520.17389595351722978No Hit
CGCGACCTCAGATCAGACGC219380.16621337697198443No Hit
TGAGGTAGTAGTTTGTGCTGTC198750.15058304619008983No Hit
CTAGACTGAAGCTCCTTGAGG195560.1481661409455797No Hit
TGAGATGAAGCACTGTAGCTC194860.14763578556277185No Hit
TGAGGTAGTAGGTTGTATAGTC191280.14492339660498305No Hit
ACCAATATTACTGTGCTGCTT190680.14446880627686204No Hit
TGAGGTAGTAGATTGTATAG190310.14418847557452075No Hit
TGTAAACATCCCCGACTGGAAG189320.14343840153312104No Hit
CGCGACCTCAGATCAGACG184960.1401350451487749No Hit
GCATTGGTGGTTCAGTGGTAGAATT183710.13918798196518944No Hit
TGAGGTAGTAGGTTGTATAGC182320.1381348477050424No Hit
TGGCTCAGTTCAGCAGGAACAG175750.13315708361211717No Hit
TGAGGTAGTAGATTGTATAGTC173090.1311417331574473No Hit
AAACCGTTACCATTACTGAGG172710.13085382594963732No Hit
TAGCTTATCAGACTGATGTTGAT170460.12914911221918346No Hit
CAACGGAATCCCAAAAGCAGCT169830.12867179237465637No Hit
TCAGTGCATCACAGAACTTTGT166250.1259594034168676No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT162190.12288334219658198No Hit
CAACGGAATCCCAAAAGCAGCTG161520.12237571633018018No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT160710.12176201938721679No Hit
TGAGGGGCAGAGAGCGAGACTT157790.11954967979036113No Hit
CCCATAAAGTAGAAAGCACC155190.11757978836850334No Hit
TGAGGTAGTAGGTTGTGTGGC154250.11686759685444706No Hit
AACCCGTAGATCCGATCTTGT153770.11650392459195025No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT153470.11627662942788974No Hit
TGAGGTAGTAGTTTGTACAGC153170.11604933426382921No Hit
AGACCGTTACCATTACTGAGT152470.11551897888102136No Hit
TGAGGTAGTAGGTTGTGTGGTC150940.11435977354431275No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT148410.11244291766073576No Hit
TGAGGTAGTAGTTTGTGCTG143870.10900318417795332No Hit
CATTGCACTTGTCTCGGTCT141960.10755607163343472No Hit
TTCAAGTAATTCAGGATAGGTT141910.10751818910609132No Hit
TATTGCACTTGTCCCGGCCTG140250.10626048919828981No Hit
AGCTCGGTCTGAGGCCCCTCAGT139230.10548768564048407No Hit
TACAGTACTGTGATAACTGA138060.10460123450064807No Hit
AAACCGTTACTATTACTGAGT137440.10413149116158966No Hit
TGAGGTAGTAGATTGTATAGC135880.102949556308475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)