FastQCFastQC Report
Wed 8 May 2024
EGAF00002167699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11894405
Total Bases265 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT141996211.938066679249614No Hit
TGGAGTGTGACAATGGTGTTT2505472.106427349665662No Hit
AAACCGTTACCATTACTGAGC2254351.8953028755957106No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2109511.7735313367923826No Hit
TCAGTGCACTACAGAACTTTGT1924751.618197799721802No Hit
TTCAAGTAATCCAGGATAGGCT1767091.4856480841202229No Hit
TAGCTTATCAGACTGATGTTGAC1702081.4309921345372048No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1423411.1967055098594674No Hit
TCCTGTACTGAGCTGCCCCGA1385751.165043564600331No Hit
CGCGACCTCAGATCAGA1298531.0917149701897657No Hit
TATTGCACTTGTCCCGGCCTGT1203221.0115848585952807No Hit
TCCTGTACTGAGCTGCCCCGAG1084760.9119918146388996No Hit
TAGCTTATCAGACTGATGTTGA1084310.9116134855001155No Hit
TAGCTTATCAGACTGATGTTG1067790.897724602449639No Hit
AAACCGTTACCATTACTGAG904770.7606685664394309No Hit
AAGCTGCCAGTTGAAGAACTGT874160.734933777687913No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG806780.6782852946406315No Hit
TGAGGTAGTAGTTTGTGCTGTT794070.6675995982985278No Hit
TCGTACCGTGAGTAATAATGCG769030.6465476835537382No Hit
TGAGGTAGTAGGTTGTATAGT731110.6146671481255263No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC727490.6116237003868626No Hit
TGAGGGGCAGAGAGCGAGACTTT685530.5763466100237885No Hit
TGAGGTAGTAGATTGTATAGTT658150.5533273837573213No Hit
TGAGGTAGTAGGTTGTATAGTT648250.5450041427040697No Hit
TTCACAGTGGCTAAGTTCCG579680.487355189267559No Hit
TGGCTCAGTTCAGCAGGAAC575290.4836643783358646No Hit
TGAGGTAGTAGGTTGTGTGGT562870.47322249410542183No Hit
TGAGGTAGTAGATTGTATAGT559270.4701958609951486No Hit
TGAGGTAGTAGGTTGTGTGGTT529820.44543632069027406No Hit
TGAGGTAGTAGGTTGTGTGG515930.4337585612731364No Hit
TGAGGGGCAGAGAGCGAGACT510090.42884868978313756No Hit
AAACCGTTACCATTACTG470190.39530350614427545No Hit
CCCATAAAGTAGAAAGCACT467200.3927897191999095No Hit
TCCTGTACTGAGCTGCCCCG411490.3459525718184306No Hit
TGAGGTAGTAGTTTGTACAGT409770.3445065137768556No Hit
CATTGCACTTGTCTCGGTCTGA371860.31263438566283897No Hit
TTCAAGTAATCCAGGATAGGCC345910.29081740532628575No Hit
TGAGGTAGTAGTTTGTGCTGT339160.28514246824452333No Hit
TCAGTGCACTACAGAACTTTGC335930.2824269057594726No Hit
TACAGTACTGTGATAACTGAAG324150.2725230896375228No Hit
AAAAGCTGGGTTGAGAGGGCGA320680.26960575161178724No Hit
TGAGGTAGTAGGTTGTATAG296420.24920960737422343No Hit
TGGAGTGTGACAATGGTGTTC290360.24411477497193007No Hit
TGGAGTGTGACAATGGTGTTTG283500.23834735743402044No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT273330.22979711889749843No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC256210.21540379699530998No Hit
CTAGACTGAAGCTCCTTGAGG253790.2133692269600707No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC253450.2130833782774338No Hit
TGTAAACATCCCCGACTGGAAG251010.21103199361380415No Hit
TGAGAACTGAATTCCATGGGTT249140.20945982585930106No Hit
TCCTGTACTGAGCTGCCCCGAGA241710.20321319141226485No Hit
CGCGACCTCAGATCAGACGT240480.20217909176625481No Hit
TGTAAACATCCCCGACTGGAAGCT238450.20047240698462848No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC229970.19334300454709588No Hit
TATTGCACTTGTCCCGGCCTG227650.19139250765380866No Hit
CATTGCACTTGTCTCGGTCTG221670.18636493376507693No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC221470.18619678748117285No Hit
TATTGCACTTGTCCCGGCCTGC220370.18527198291970048No Hit
TACAGTATAGATGATGTACT213260.17929438252691077No Hit
TACAGTACTGTGATAACTGA213260.17929438252691077No Hit
TGAGGTAGTAGATTGTATAG213070.17913464355720188No Hit
AACCCGTAGATCCGATCTTGT210050.17659563467025044No Hit
TCCTGTACTGAGCTGCCCCGAGT198470.16685996483220472No Hit
AGGAAACTCTGGTG197800.16629667478112609No Hit
TGGCTCAGTTCAGCAGGAACAG191330.1608571424968294No Hit
CGCGACCTCAGATC191240.16078147666907255No Hit
ACCAATATTACTGTGCTGCTT183740.1544759910226699No Hit
TTCAAGTAATTCAGGATAGGT176960.1487758319983219No Hit
TGAGGTAGTAGTTTGTGCTG175700.14771651040972625No Hit
AAAAGCTGGGTTGAGAGGGCG173520.14588371591517188No Hit
TGTAAACATCCCCGACTGGAAGC169760.14272256577777537No Hit
TGAGGGGCAGAGAGCGAGACTT169250.14229379275381995No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT166780.1402171861476047No Hit
AAACCGTTACCATTACTGAGA162310.13645911670234873No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG158550.13329796656495219No Hit
TGTAAACATCCTTGACTGGAAGCT158080.1329028227977776No Hit
AAGCTGCCAGTTGAAGAACTGC155270.13054036750892542No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT154920.13024611151209328No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT154800.13014522374175083No Hit
TAGCTTATCAGACTGATGTTGAT147640.12412558677798512No Hit
AAGCTGCCAGTTGAAGAACT147510.12401629169344747No Hit
TGAGATGAAGCACTGTAGCTC146850.12346140895656403No Hit
TGAGGTAGTAGTTTGTACAGTT143530.12067018064375647No Hit
TCGTACCGTGAGTAATAATGC139430.117223181823723No Hit
CGGACAGGATTGACAGA135990.11433106574057299No Hit
TCAGTGCATCACAGAACTTTGT133970.11263278827314187No Hit
AAACCGTTACCATTACTGAGG130950.11009377938619039No Hit
CAACGGAATCCCAAAAGCAGCTG129890.10920260408149882No Hit
AGACCGTTACCATTACTGAGT129200.10862249940202977No Hit
TAGCAGCACGTAAATATTGGCG127680.10734458764435884No Hit
AGCTCGGTCTGAGGCCCCTCAGT124950.10504939086906828No Hit
AACATTCAACGCTGTCGGTGA123980.10423388139213353No Hit
TGAGGTAGTAGGTTGTATAGC123430.10377147911139734No Hit
TGAGGTAGTAGATTGTAT121840.10243471615435998No Hit
TCGTACCGTGAGTAATAATGCA120220.10107273125473701No Hit
TACAGTACTGTGATAACTGAA119100.1001311120648742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)