FastQCFastQC Report
Wed 8 May 2024
EGAF00002167700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13991207
Total Bases314.9 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10407517.438607691244938No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3093262.210860006574129No Hit
TCAGTGCACTACAGAACTTTGT3066992.1920839281414395No Hit
TTCAAGTAATCCAGGATAGGCT2370721.6944356551940087No Hit
AAACCGTTACCATTACTGAGC1922541.3741058937945811No Hit
TGAGGTAGTAGGTTGTATAGT1703431.2175003914958875No Hit
TATTGCACTTGTCCCGGCCTGT1557151.1129490114755647No Hit
TGGAGTGTGACAATGGTGTTT1454881.0398531020232922No Hit
TGAGGTAGTAGGTTGTATAGTT1447101.0342924666899718No Hit
TGAGGTAGTAGATTGTATAGTT1268820.90686957887193No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1229470.8787447716269224No Hit
TGAGGTAGTAGATTGTATAGT1202930.8597757148471894No Hit
TAGCTTATCAGACTGATGTTGAC1134610.8109450456990593No Hit
AAGCTGCCAGTTGAAGAACTGT1116890.7982799482560726No Hit
TCCTGTACTGAGCTGCCCCGAG1072790.7667601515723411No Hit
TGAGGGGCAGAGAGCGAGACTTT1061010.7583405777643059No Hit
TGAGGTAGTAGTTTGTGCTGTT1002800.7167358756110177No Hit
TCCTGTACTGAGCTGCCCCGA957980.6847014700018376No Hit
TAGCTTATCAGACTGATGTTGA938220.670578313936746No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC806520.5764477646567591No Hit
TGAGGTAGTAGGTTGTGTGGTT792730.5665915742651796No Hit
TGAGGTAGTAGGTTGTGTGGT763380.5456141132069592No Hit
TGAGGTAGTAGTTTGTACAGT709490.5070970646063632No Hit
CCCATAAAGTAGAAAGCACT618050.44174173107438125No Hit
TCAGTGCACTACAGAACTTTGC582550.4163686521112868No Hit
CATTGCACTTGTCTCGGTCTGA556080.3974496267548611No Hit
CGGACAGGATTGACAGA549670.39286817784913053No Hit
TAGCTTATCAGACTGATGTTG536510.3834622702673186No Hit
TTCAAGTAATCCAGGATAGGCC531590.37994577594341933No Hit
TGGCTCAGTTCAGCAGGAAC515720.3686029375449881No Hit
TGAGGGGCAGAGAGCGAGACT511990.36593697741731646No Hit
AAACCGTTACCATTACTGAG488840.3493908709949041No Hit
TCGTACCGTGAGTAATAATGCG484850.34653907986637605No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT464140.33173692591353987No Hit
TGAGAACTGAATTCCATGGGTT452860.3236747194148439No Hit
CGCGACCTCAGATCAGA444500.3176995380026898No Hit
TTCACAGTGGCTAAGTTCCG443500.31698480338401114No Hit
TCCTGTACTGAGCTGCCCCGAGT381440.2726283729488099No Hit
CGCGACCTCAGATC380910.2722495636009102No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT364930.2608281043944243No Hit
TATTGCACTTGTCCCGGCCTGC364330.2603992636232171No Hit
CGCGACCTCAGATCAGACGT364120.2602491693532945No Hit
TGTAAACATCCCCGACTGGAAGCT359510.25695424276118567No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG318370.22755006054874322No Hit
TGAGGTAGTAGGTTGTATAGC314370.22469112207402836No Hit
TGAGGTAGTAGTTTGTGCTGT308600.22056710332425214No Hit
TCCTGTACTGAGCTGCCCCGAGA304830.2178725538118334No Hit
TGAGGTAGTAGTTTGTACAGTT300650.21488496310575633No Hit
TGAGGTAGTAGGTTGTATAG295460.2111754904348138No Hit
AAAAGCTGGGTTGAGAGGGCGA288820.20642965256678714No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT283850.2028774215119539No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC283300.20248431747168064No Hit
TCCTGTACTGAGCTGCCCCG283270.20246287543312028No Hit
TGAGATGAAGCACTGTAGCT282520.20192682446911123No Hit
TTCAAGTAATTCAGGATAGGT275070.19660205155995475No Hit
TGAGGTAGTAGGTTGTATAGTC274540.19622324221205503No Hit
TGTAAACATCCTTGACTGGAAGCT272090.19447214239629218No Hit
TGAGGTAGTAGGTTGTGTGG259560.18551651762424784No Hit
AAACCGTTACCATTACTGAGTT255460.18258610568766512No Hit
TGTAAACATCCCCGACTGGAAG245440.17542446480850435No Hit
GCGGGGTGGAGCAGTCTGGTAGCTCGTCGGG241530.1726298524494706No Hit
CTAGACTGAAGCTCCTTGAGG240610.17197229660028615No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC239200.17096452078794916No Hit
TGGAGTGTGACAATGGTGTTC237310.16961367235864638No Hit
TGAGGTAGTAGATTGTATAGTC235300.16817705577510217No Hit
TGAGGGGCAGAGAGCGAGACTT232930.1664831347288336No Hit
AAGCTGCCAGTTGAAGAACTGC223730.15990757623698942No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC223000.15938581996535395No Hit
TGAGGTAGTAGATTGTATAGC220190.15737741568686675No Hit
GTAGTCGTGGCCGAGTGGT213610.1526744618959608No Hit
CATTGCACTTGTCTCGGTCTG211590.1512306979662298No Hit
GCGGGATGGAGCAGTCTGGTAGCTCGTCGGG209300.14959395568945555No Hit
AAACCGTTACCATTACTG205930.14718530002450825No Hit
TAGCTTATCAGACTGATGTTGAT203510.14545564224730576No Hit
TACAGTACTGTGATAACTGAAG200150.14305413392854527No Hit
AACCCGTAGATCCGATCTTGT195970.14006654322246823No Hit
AGCTCGGTCTGAGGCCCCTCAGT192350.1374792039028513No Hit
TCAGTGCATCACAGAACTTTGT192070.13727907820962124No Hit
TGAGGTAGTAGGTTGTAT191620.1369574476312158No Hit
TACAGTATAGATGATGTACT190140.1358996403955713No Hit
TGAGGTAGTAGTTTGTGCTGTC188590.13479180173661928No Hit
TGAGGTAGTAGATTGTATAG186740.13346954269206368No Hit
TGGCTCAGTTCAGCAGGAACAG184780.13206866283945337No Hit
TTCAAGTAATCCAGGATAGGCA182910.13073210910252417No Hit
TGAGGTAGTAGATTGTAT181270.12955994432789109No Hit
ACCAATATTACTGTGCTGCTT180560.12905248274862918No Hit
TGTAAACATCCCCGACTGGAAGC178990.1279303493973036No Hit
TGAGATGAAGCACTGTAGCTC178850.1278302865506886No Hit
GTTCTATTTTGTTGGTTTTCGGAAC176190.1259290924650032No Hit
CAACGGAATCCCAAAAGCAGCT174300.12457824403570043No Hit
AAATGGATTTTTGGAGCAGGGAGA167880.11998964778378304No Hit
TGAGGTAGTAGGTTGTGTGGTC163470.11683766811540991No Hit
TGAGGTAGTAGGTTGTGTGGC158490.1132782897143899No Hit
TGAGGGGCAGAGAGCGAGACTTC155960.11147001112913274No Hit
GTCTGAGCGTCGCT149770.10704580383951148No Hit
TACCCTGTAGAACCGAATTTGT144600.10335062586094251No Hit
AAACCGTTACCATTACTGAGA143480.10255012308802236No Hit
CGGTCGGGCTGGGGCG142760.10203551416257368No Hit
CGCGACCTCAGATCAGAC140820.10064892900233696No Hit
CAACGGAATCCCAAAAGCAGCTG140750.10059889757902947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)