FastQCFastQC Report
Thu 9 May 2024
EGAF00002167701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13586987
Total Bases305 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT13094319.637390541405537No Hit
TGAGGTAGTAGGTTGTATAGT3021222.223612931991471No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2674901.9687219837628458No Hit
TCAGTGCACTACAGAACTTTGT2526531.8595219087204544No Hit
TTCAAGTAATCCAGGATAGGCT2380691.7521839095010543No Hit
TGAGGTAGTAGGTTGTATAGTT2238881.6478119836281582No Hit
TCCTGTACTGAGCTGCCCCGAG2153491.5849650846063223No Hit
TATTGCACTTGTCCCGGCCTGT2021461.4877912225867296No Hit
TGAGGTAGTAGATTGTATAGT1936371.4251651230695959No Hit
AAACCGTTACCATTACTGAGC1884581.3870477685744456No Hit
TGAGGTAGTAGATTGTATAGTT1689931.2437856899399402No Hit
TCCTGTACTGAGCTGCCCCGA1610331.1852002213588635No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1537811.1318256210887667No Hit
TGAGGTAGTAGTTTGTGCTGTT1252190.921609772644958No Hit
CGCGACCTCAGATCAGA1229480.9048952501389749No Hit
TGAGGTAGTAGGTTGTGTGGT1183610.8711350058699548No Hit
TGAGGTAGTAGGTTGTGTGGTT1183150.8707964466294109No Hit
TGAGGTAGTAGTTTGTACAGT1111110.8177751255668383No Hit
TAGCTTATCAGACTGATGTTGAC888860.6541994924996984No Hit
AAGCTGCCAGTTGAAGAACTGT881710.6489371043042876No Hit
CGCGACCTCAGATC841050.6190114114336019No Hit
TGAGGGGCAGAGAGCGAGACTTT783620.5767430262500435No Hit
CATTGCACTTGTCTCGGTCTGA781260.5750060701463835No Hit
TCCTGTACTGAGCTGCCCCGAGT728780.5363808767904172No Hit
CCCATAAAGTAGAAAGCACT697740.5135354880371933No Hit
CGCGACCTCAGATCAGACGT685240.504335508674587No Hit
TCCTGTACTGAGCTGCCCCGAGA659060.4850670718975443No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT643580.47367381745489273No Hit
TGAGGTAGTAGTTTGTACAGTT579810.4267392027386204No Hit
TAGCTTATCAGACTGATGTTGA559850.4120486756924107No Hit
TGAGGGGCAGAGAGCGAGACT558140.4107901185156061No Hit
TTCAAGTAATTCAGGATAGGT551550.4059398893956401No Hit
AAACCGTTACCATTACTGAG521070.3835066597178609No Hit
TCCTGTACTGAGCTGCCCCG505850.3723047648459515No Hit
TGAGGTAGTAGGTTGTATAG504120.37103148770216676No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC476960.3510417725430958No Hit
TCGTACCGTGAGTAATAATGCG459970.33853716059344136No Hit
TTCAAGTAATCCAGGATAGGCC439390.3233903145708464No Hit
TGAGGTAGTAGGTTGTATAGC434940.3201151219177585No Hit
TGTAAACATCCCCGACTGGAAGCT396540.291852785315832No Hit
TATTGCACTTGTCCCGGCCTGC389640.2867743967076733No Hit
TCAGTGCACTACAGAACTTTGC387420.2851404803728744No Hit
AAACCGTTACCATTACTGAGTT382190.28129120900755994No Hit
CAACGGAATCCCAAAAGCAGCT337180.2481639233186872No Hit
AAAAGCTGGGTTGAGAGGGCGA318290.2342609145059166No Hit
AGAGGTAGTAGGTTGCATAGT316140.2326785180555483No Hit
TGAGGTAGTAGGTTGTATAGTC314760.23166284033391657No Hit
TGAGGTAGTAGGTTGTAT305480.22483277565511764No Hit
TGGCTCAGTTCAGCAGGAAC300630.22126318366242642No Hit
TACAGTATAGATGATGTACT296280.2180615908442394No Hit
TAGCTTATCAGACTGATGTTG292590.21534575693639807No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC286520.21087824695791643No Hit
TGAGGTAGTAGATTGTATAG285430.21007600875749716No Hit
CATTGCACTTGTCTCGGTCTG282360.20781649382604103No Hit
TGAGGTAGTAGATTGTATAGC274810.20225970629102688No Hit
TTCACAGTGGCTAAGTTCCG274680.20216402650565574No Hit
TGAGGTAGTAGATTGTAT271100.19952915241620528No Hit
AAACCGTTACCATTACTG268380.19752723690690216No Hit
TGAGGTAGTAGTTTGTGCTGT261690.1926034079520353No Hit
AGAGGTAGTAGGTTGCATAGTT260790.19194100943792763No Hit
TGTAAACATCCTTGACTGGAAGCT255570.18809909805610325No Hit
TGAGGTAGTAGGTTGTGTGG250820.18460310589831283No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG243590.1792818378349814No Hit
TGAGGTAGTAGATTGTATAGTC233930.17217209378355924No Hit
TGAGAACTGAATTCCATGGGTT221220.16281755476766113No Hit
TGAGGGGCAGAGAGCGAGACTT220780.1624937154940974No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC220650.1623980357087263No Hit
TATTGCACTTGTCCCGGCCTG209670.15431677383661294No Hit
TGTAAACATCCCCGACTGGAAG204600.1505852622071398No Hit
CAACGGAATCCCAAAAGCAGCTG203640.14987870379209164No Hit
TGAGGTAGTAGGTTGTGTGGC201620.14839198712709448No Hit
AGCTCGGTCTGAGGCCCCTCAGT200290.14741310932291318No Hit
ACCAATATTACTGTGCTGCTT196330.14449855586083948No Hit
TGAGGTAGTAGGTTGTGTGGTC190030.13986176626208593No Hit
GCATTGGTGGTTCAGTGGTAGAATT189940.13979552641067516No Hit
TGAGATGAAGCACTGTAGCT184060.13546785611850515No Hit
TGAGGTAGTAGTTTGTGCTGTC182490.1343123387105618No Hit
TAGCAGCACGTAAATATTGGC179270.1319424240267544No Hit
TGGAGTGTGACAATGGTGTTT176840.13015394803866376No Hit
TACAGTACTGTGATAACTGAAG171620.12631203665683938No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT170510.12549507848943994No Hit
TGAGGTAGTAGTTTGTACAGC167450.12324292354147391No Hit
GTTTCCGTAGTGTAGTGGTTATC163160.12008549062422742No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT162430.11954821182945123No Hit
TCCTGTACTGAGCTGCCCCGAA162370.11950405192851071No Hit
TTCAAGTAATTCAGGATAGGTT162370.11950405192851071No Hit
TATTGCACTTGTCCCGGCCTGTA160680.11826021471868635No Hit
AAGCTGCCAGTTGAAGAACT158820.11689125778953052No Hit
CTAGACTGAAGCTCCTTGAGG155800.11466854277552485No Hit
CGCGACCTCAGATCAGACG152800.11246054772849932No Hit
CGCGACCTCAGATCAGAC152690.1123795879101084No Hit
TGTAAACATCCCCGACTGGAAGC147340.10844199674291291No Hit
GGCTGGTCCGATGGTAGTGGGT147290.10840519682546249No Hit
GGCTGGTCCGATGGTAGT145430.10703623989630666No Hit
TGGAGAGAAAGGCAGTTCCTGA144970.10669768065576277No Hit
TAGCAGCACGTAAATATTGGCG144860.10661672083737182No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT143100.10532136374311685No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC142510.10488712471720184No Hit
TTTGGCAATGGTAGAACTCACACT139840.10292200912534913No Hit
AAGCTGCCAGTTGAAGAACTGC138560.10197993123861825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)