FastQCFastQC Report
Wed 8 May 2024
EGAF00002167702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12605487
Total Bases296.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10599858.408917481728393No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3768162.989301405015133No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3240782.570928041098293No Hit
TTCAAGTAATCCAGGATAGGCT2656432.107360072641382No Hit
TATTGCACTTGTCCCGGCCTGT2140221.6978479292390687No Hit
AAACCGTTACCATTACTGAGC1781511.4132813750075663No Hit
TCAGTGCACTACAGAACTTTGT1574841.24932896285562No Hit
TAGCTTATCAGACTGATGTTGAC1392341.1045507404830928No Hit
TCCTGTACTGAGCTGCCCCGAG1346651.0683046200436366No Hit
TGAGGTAGTAGATTGTATAGTT1295871.0280205754843108No Hit
TCCTGTACTGAGCTGCCCCGA1259890.9994774497804011No Hit
TGAGGTAGTAGGTTGTATAGTT1241270.984706104571763No Hit
TGAGGTAGTAGGTTGTATAGT923430.7325619390984259No Hit
AAGCTGCCAGTTGAAGAACTGT895520.7104207873920301No Hit
TGAGGTAGTAGTTTGTGCTGTT762200.6046573210539188No Hit
TGAGGTAGTAGATTGTATAGT753400.5976762341669148No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC748380.5936938414200102No Hit
TGAGGGGCAGAGAGCGAGACTTT701880.5568051436648184No Hit
CGCGACCTCAGATC695480.5517279895651791No Hit
CGCGACCTCAGATCAGA678040.5378927446436619No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC645750.5122769156003255No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT624060.49507012303451664No Hit
TTCAAGTAATCCAGGATAGGCC576760.45754678101686985No Hit
TAGCTTATCAGACTGATGTTGA562900.4465515691698385No Hit
TGTAAACATCCCCGACTGGAAGCT559260.4436639377756686No Hit
TGAGGTAGTAGTTTGTACAGT548770.4353421648842286No Hit
CGCGACCTCAGATCAGACGT507320.4024596590357834No Hit
CCCATAAAGTAGAAAGCACT485750.3853480631093428No Hit
TATTGCACTTGTCCCGGCCTGC477510.3788112272060572No Hit
AAACCGTTACCATTACTGAG469900.3727741736594548No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT424540.3367898439782612No Hit
TGAGGGGCAGAGAGCGAGACT420760.33379114983816177No Hit
CATTGCACTTGTCTCGGTCTGA413170.3277699623981208No Hit
TGAGGTAGTAGTTTGTACAGTT408030.32369237301184794No Hit
TCCTGTACTGAGCTGCCCCGAGT391360.31046797319294367No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT368320.29219021843424214No Hit
TCGTACCGTGAGTAATAATGCG358370.2842968304199592No Hit
TCCTGTACTGAGCTGCCCCG355360.28190898138247256No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG345460.274055258634593No Hit
TGAGGTAGTAGGTTGTGTGGTT343250.2723020538595613No Hit
TGAGAACTGAATTCCATGGGTT338210.26830379500609536No Hit
TCCTGTACTGAGCTGCCCCGAGA335460.26612220535390657No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT323850.2569119304950297No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC315570.2503433623786213No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT298120.2365001844038235No Hit
TGAGGTAGTAGGTTGTGTGGT294140.2333428291981103No Hit
AAACCGTTACCATTACTGAGTT293030.23246226028395411No Hit
TCAGTGCACTACAGAACTTTGC285110.22617928208565047No Hit
TGTAAACATCCTTGACTGGAAGCT278980.22131632042458968No Hit
AAAAGCTGGGTTGAGAGGGCGA264790.21005931781929568No Hit
TGAGGTAGTAGTTTGTGCTGT254140.20161061607536462No Hit
TAGCTTATCAGACTGATGTTG252010.19992087572657846No Hit
TAGCTTATCAGACTGATGTTGAT249990.1983183989638798No Hit
GCATTGGTGGTTCAGTGGTAGAATT239150.18971896920761572No Hit
TTCAAGTAATTCAGGATAGGT236880.18791816611289988No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC222570.1765659668682376No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA220100.17460650270790806No Hit
AGCTCGGTCTGAGGCCCCTCAGT217890.17285329793287638No Hit
CAACGGAATCCCAAAAGCAGCT212470.16855358305474433No Hit
TGAGGTAGTAGATTGTATAGTC211810.16803000153821904No Hit
TGAGGTAGTAGGTTGTATAGTC208900.16572148303353929No Hit
GGCTGGTCCGATGGTAGTGGGT204360.16211987684410764No Hit
TGAGGTAGTAGGTTGTATAG200120.1587562622530966No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT199060.15791535860534384No Hit
TGGCTCAGTTCAGCAGGAAC198360.1573600448756958No Hit
CAACGGAATCCCAAAAGCAGCTG195620.1551863882767877No Hit
TGAGGGGCAGAGAGCGAGACTT193010.15311586137052857No Hit
AAACCGTTACCATTACTG186590.14802284116432787No Hit
TATTGCACTTGTCCCGGCCTG185850.1474357952215571No Hit
TGTAAACATCCCCGACTGGAAG183020.14519074114312283No Hit
AGAGGTAGTAGGTTGCATAGTT181830.14424670780272114No Hit
GGCTGGTCCGATGGTAGT180340.14306468286389887No Hit
AAGCTGCCAGTTGAAGAACTGC169420.1344017886813893No Hit
CGCGACCTCAGATCAGAC169280.1342907259354597No Hit
CATTGCACTTGTCTCGGTCTG166930.13242645841449838No Hit
TACAGTACTGTGATAACTGAAG164620.13059392310665982No Hit
GGCTGGTCCGATGGTAGTGGGTT163540.12973715335234567No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT162760.12911837519645214No Hit
TGTAAACATCCCCGACTGGAAGC161270.12793635025762987No Hit
TTCACAGTGGCTAAGTTCCG157310.12479486115847804No Hit
TGAGGTAGTAGGTTGTATAGC156280.12397775667056736No Hit
TTCAAGTAATCCAGGATAGGCA154000.12216902052257085No Hit
CTAGACTGAAGCTCCTTGAGG153490.12176443480525583No Hit
ACCAATATTACTGTGCTGCTT150890.11970184095227737No Hit
TAGCTTATCAGACTGATGTTGACT148520.1178217073247547No Hit
TGGAGTGTGACAATGGTGTTT143330.11370445267207845No Hit
TCAGTGCATCACAGAACTTTGT141340.11212577506922183No Hit
AAGCTGCCAGTTGAAGAACT139800.11090408486399614No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT136190.10804025262966833No Hit
TTCAAGTAATTCAGGATAGGTT135300.10733421088768724No Hit
AAACCGTTACCATTACTGAGG134920.10703275486302116No Hit
TGAGGTAGTAGATTGTATAG133490.105898328243883No Hit
TGAGGTAGTAGTTTGTGCTGTC131450.10427998537462298No Hit
TGAGGTAGTAGATTGTAT130000.10312969264892344No Hit
TGTAAACATCCCCGACTGGAAGCC126520.10036899010724457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)