FastQCFastQC Report
Wed 8 May 2024
EGAF00002167704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17304945
Total Bases409.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT15465398.936977262857525No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC7476704.320556927514072No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT4299402.4844921494983083No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC4108972.374448459674388No Hit
TCAGTGCACTACAGAACTTTGT3002201.7348798277024284No Hit
TCCTGTACTGAGCTGCCCCGA2847231.6453273905233445No Hit
TCCTGTACTGAGCTGCCCCGAG2354981.3608711267212925No Hit
TTCAAGTAATCCAGGATAGGCT2343221.3540753813433095No Hit
AAACCGTTACCATTACTGAGC1989971.149942978726601No Hit
TAGCTTATCAGACTGATGTTGAC1940161.1211592986860115No Hit
TATTGCACTTGTCCCGGCCTGT1905771.1012863664114505No Hit
AAGCTGCCAGTTGAAGAACTGT1247210.7207246252444027No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1100420.6358991606156507No Hit
TAGCTTATCAGACTGATGTTGA1050650.6071385953552583No Hit
TGAGGGGCAGAGAGCGAGACTTT992460.5735123688633509No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC880820.5089990173329069No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT851020.4917785060859771No Hit
TCGTACCGTGAGTAATAATGCG850710.49159936653944875No Hit
ATTTTAGCGGAACGCTCTGGAAAGTGC850640.4915589156741036No Hit
AAACCGTTACCATTACTGAG847960.49001022540088973No Hit
TGAGGGGCAGAGAGCGAGACT838410.48449157162880324No Hit
CATTGCACTTGTCTCGGTCTGA824350.47636672638948No Hit
TCCTGTACTGAGCTGCCCCG808230.4670514699699999No Hit
TGAGGTAGTAGGTTGTATAGTT800500.4625845386968869No Hit
TGAGGTAGTAGATTGTATAGTT684790.39571925828137566No Hit
CGCGACCTCAGATCAGA680430.39319974723987855No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT674570.3898134319409856No Hit
TGGAGTGTGACAATGGTGTTT634690.36676799608435623No Hit
TGAGGTAGTAGTTTGTGCTGTT615750.35582314766097206No Hit
AAAAGCTGGGTTGAGAGGGCGA598860.3460629317226955No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC589400.34059628620605265No Hit
TAGCTTATCAGACTGATGTTG552830.3194635984107433No Hit
TTCACAGTGGCTAAGTTCCG550720.31824429375533986No Hit
TCCTGTACTGAGCTGCCCCGAGA546660.3158981435653219No Hit
TGAGGTAGTAGGTTGTATAGT522510.3019425950212497No Hit
CCCATAAAGTAGAAAGCACT517920.29929017399361857No Hit
CGCGACCTCAGATC502130.29016561451076556No Hit
TGGCTCAGTTCAGCAGGAAC493110.2849532315762922No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG492170.2844100342416575No Hit
CTTATCGGATTGGACATGCTTCGAAGTG472260.2729046523984907No Hit
TGAGGTAGTAGGTTGTGTGGTT434720.2512114311833987No Hit
CTAGACTGAAGCTCCTTGAGG427690.24714900856373712No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT426890.24668671295979272No Hit
TCAGTGCACTACAGAACTTTGC417250.24111605093226243No Hit
TGTAAACATCCCCGACTGGAAG400200.23126337587319692No Hit
TGTAAACATCCCCGACTGGAAGCT398080.23003829252274421No Hit
TGAGGTAGTAGGTTGTGTGGT394480.22795796230499432No Hit
AAACCGTTACCATTACTG390080.22541533648330003No Hit
TGAGAACTGAATTCCATGGGTT385250.22262422677448557No Hit
TGAGGTAGTAGTTTGTACAGT379520.21931303451123366No Hit
TTCAAGTAATCCAGGATAGGCC376490.21756208991129414No Hit
AAGACTTATCAGAACGGCTTGGAAAGGCC376430.2175274177409983No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC364510.21063921324222645No Hit
TGAGGTAGTAGATTGTATAGT364080.2103907293551063No Hit
TCCTGTACTGAGCTGCCCCGAGT360040.20805613655518698No Hit
CGCGACCTCAGATCAGACGT359630.20781921005816545No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA330630.19106099441518015No Hit
TGAGGTAGTAGGTTGTGTGG329550.1904368953498552No Hit
TGGCTCAGTTCAGCAGGAACAG305930.17678761764339615No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC301370.17415253270091294No Hit
CATTGCACTTGTCTCGGTCTG293690.1697144949030465No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA292560.169061502362475No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT290150.16766883685559242No Hit
TATTGCACTTGTCCCGGCCTGC289200.16711986082590843No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA285470.16496440757251757No Hit
TGAGGTAGTAGTTTGTACAGTT279600.16157231357857538No Hit
TGTAAACATCCCCGACTGGAAGC278160.16074018149147543No Hit
TGTAAACATCCTTGACTGGAAGCT277170.16016809068159418No Hit
AGGAAACTCTGGTG275690.159312843814297No Hit
CAACGGAATCCCAAAAGCAGCTG269010.1554526755213611No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT267220.15441828910753544No Hit
TATTGCACTTGTCCCGGCCTG258280.14925213573345653No Hit
AAACCGTTACCATTACTGAGTT254700.14718336290580525No Hit
TACAGTACTGTGATAACTGAAG252550.14594094347020461No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC243520.14072278184068196No Hit
TGAGGTAGTAGGTTGTATAG241990.13983864149813827No Hit
GCATTGGTGGTTCAGTGGTAGAATT241350.1394688050149827No Hit
TAGCAGCACGTAAATATTGGCG241170.13936478850409523No Hit
AGCTCGGTCTGAGGCCCCTCAGT227630.13154043540733587No Hit
TGAGGTAGTAGTTTGTGCTGT224930.12998018774402345No Hit
TGAGGGGCAGAGAGCGAGACTT218070.12601600294020004No Hit
TGAGATGAAGCACTGTAGCT212860.123005302819512No Hit
AAGCTGCCAGTTGAAGAACT207720.12003505356416909No Hit
TGAGATGAAGCACTGTAGCTC197900.1143603750257513No Hit
AACATTCAACGCTGTCGGTGA195990.113256644271334No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG193460.11179463442385978No Hit
TCAGTGCATCACAGAACTTTGT183460.1060159393745545No Hit
AAAAGCTGGGTTGAGAGGGCG182280.10533405335873647No Hit
AAGCTGCCAGTTGAAGAACTGC179290.10360622353899421No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT179050.10346753485781088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)